Expression of functional PDGF β receptors in a human large‐cell lung‐carcinoma cell line

Abstract

In this study we have investigated a panel of lung-cancer cell lines, both of small-cell carcinoma and non-small-cell type, for the expression of receptors for platelet-derived growth factor. Although we found mRNA expression for the beta-type receptor on one small-cell and one non-small-cell line and alpha-type receptor mRNA expression on one small-cell-cancer cell line, only the beta receptors of the non-small-cell line (H-157) proved to be functional. Thus, the cell line H-157 displayed specific binding of 125I-PDGF-BB in addition to mRNA expression of the 6-kb transcript for the PDGF beta type receptor. Further evidence for the presence of functional PDGF beta receptors in H-157 cells was obtained from an in vitro kinase assay, which demonstrated a ligand-induced receptor autophosphorylation as well as the phosphorylation of a number of potential substrates associated with the activated receptor.

Function and regulation of melanoma–stromal fibroblast interactions: when seeds meet soil

“…Thus, melanoma-derived bFGF has potential paracrine functions in stroma formation (Lazar-Molnar et al, 2000). PDGF, on the other hand, is not produced for autocrine growth because the melanoma cells do not express the PDGF b receptor (Westermark et al, 1986;Forsberg et al, 1993). Besides its mitogenic activity, (Ono et al, 1995;Pierce et al, 1995).…”