Expression of genes, encoded defense proteins, in potato plants infected with the cyst-forming nematode Globodera rostochiensis (Wollenweber 1923) Behrens, 1975 and modulation of their activity during short-term exposure to low temperatures

Lavrova, V V; Матвеева, Е. М.; Zinovieva, S. V.

doi:10.1134/s1062359017020108

Cited by 14 publications

(5 citation statements)

References 32 publications

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“…Previous studies have shown that the level of the PR-6 gene transcripts and the dynamics of their activity under the root-knot (M. incoignita) and cyst-forming (Globodera rostochiensis) nematode invasion of tomato or potato plants, respectively, were different in nematode-resistant plants. The increased activity of these genes in the period of nematode development and reproduction indicated the potential role of the enzymes of this group as one of the main components of plant defense against invasion (Lavrova et al, 2017a(Lavrova et al, , 2017bSeiml-Buchinger et al, 2019). The data of the present study demonstrate the potential involvement of two PI classes, serine and cysteine, in this process, as well as the modulation of their activity by the biogenic elicitors: jasmonic and salicylic acids.…”

Section: Discussionsupporting

confidence: 57%

Gene Expression of Protease Inhibitors in Tomato Plants with Invasion by Root-Knot Nematode Meloidogyne incognita and Modulation of Their Activity with Salicylic and Jasmonic Acids

Zinovieva

Udalova

Seiml-Buchinger

et al. 2021

Biol Bull Russ Acad Sci

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Abstract— The expression of the genes encoding the inhibitors of serine (ISP) and cysteine proteinases (ICP) was studied in the roots of tomato plants resistant and susceptible to the root-knot nematode Meloidogyne incognita during infection and under the effects of signaling molecules: salicylic (SA) and jasmonic (JA) acids. It was shown that, upon infection, resistant plants are characterized by an increased accumulation of transcripts of the ICP and ISP genes at the stages of penetration and development in the roots, while the level of transcription does not change in susceptible plants. There was a significant decrease in nematode invasion in susceptible plants after treatment with SA or JA compared to untreated plants, which makes it possible to determine the role of the studied proteinase inhibitors in resistance induced by signaling molecules. It was revealed that an increase in expression of the genes of proteinase inhibitors is accompanied by inhibition of the reproductive potential and size of M. incognita females, as well as by a decrease in plant infection.

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Section: Discussionsupporting

confidence: 57%

Gene Expression of Protease Inhibitors in Tomato Plants with Invasion by Root-Knot Nematode Meloidogyne incognita and Modulation of Their Activity with Salicylic and Jasmonic Acids

Zinovieva

Udalova

Seiml-Buchinger

et al. 2021

Biol Bull Russ Acad Sci

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“…The golden PCN, G. rostochiensis , and the pale PCN, G. pallida , are worm-like microscopic endoparasites which feed on potato roots, deteriorating the quality of tubers and reducing their commercial value. In addition, PCN may facilitate the infection of potatoes by opportunistic pathogens, like bacteria and fungi [ 6 ], significantly reducing yield, increasing the overall costs of production and imposing trade restrictions. Therefore, upon PCN detection, crop fields are subjected to severe quarantine in many countries, where these nematodes are considered harmful quarantine organisms [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida

Camacho

Albuquerque

Andrade

et al. 2023

Sensors

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The potato cyst nematode (PCN), Globodera pallida, has acquired significant importance throughout Europe due to its widespread prevalence and negative effects on potato production. Thus, rapid and reliable diagnosis of PCN is critical during surveillance programs and for the implementation of control measures. The development of innovative technologies to overcome the limitations of current methodologies in achieving early detection is needed. Lab-on-a-chip devices can swiftly and accurately detect the presence of certain nucleotide sequences with high sensitivity and convert the presence of biological components into an understandable electrical signal by combining biosensors with microfluidics-based biochemical analysis. In this study, a specific DNA-probe sequence and PCR primers were designed to be used in a magnetoresistive biosensing platform to amplify the internal transcribed spacer region of the ribosomal DNA of G. pallida. Magnetic nanoparticles were used as the labelling agents of asymmetric PCR product through biotin–streptavidin interaction. Upon target hybridization to sensor immobilized oligo probes, the fringe field created by the magnetic nanoparticles produces a variation in the sensor’s electrical resistance. The detection signal corresponds to the concentration of target molecules present in the sample. The results demonstrate the suitability of the magnetic biosensor to detect PCR target product and the specificity of the probe, which consistently distinguishes G. pallida (DV/V > 1%) from other cyst nematodes (DV/V < 1%), even when DNA mixtures were tested at different concentrations. This shows the magnetic biosensor’s potential as a bioanalytical device for field applications and border phytosanitary inspections.

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“…Beyond Europe, PCN have been reported throughout South America and parts of Asia, North America, Oceania and Africa where potatoes are grown [5]. The golden potato cyst nematode, G. rostochiensis, and the pale potato cyst nematode, G. pallida, are sedentary endoparasites of the potato root system that deteriorate the quality and commercial value of tubers and contribute to infection of potatoes by other opportunistic pathogens, such as fungi and bacteria [6]. Therefore, PCN are considered harmful quarantine organisms and are subject to strict quarantine regulations in many countries [7].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, Globodera pallida

et al. 2021

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The potato cyst nematode (PCN) Globodera pallida has acquired significant importance throughout Europe due to its nefarious effects on potato production. Rapid and reliable diagnosis of PCN is critical during the surveillance programs and for the implementation of control measures. Molecular DNA-based methods are available, but they require expensive laboratory facilities, equipment and trained technicians. Moreover, there is an additional need of time for sample shipment and testing. In this work, we have developed a new and simple assay which reliably discriminates G. pallida from other cyst nematodes in less than 40 min. This assay may be applied either on cysts or juveniles with the ability to detect a single juvenile of G. pallida in a sample of at least 40 juveniles of the non-target species G. rostochiensis. This test should be a tool to improve the performance of the laboratory and has the potential to be performed on-site.

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Expression of genes, encoded defense proteins, in potato plants infected with the cyst-forming nematode Globodera rostochiensis (Wollenweber 1923) Behrens, 1975 and modulation of their activity during short-term exposure to low temperatures

Cited by 14 publications

References 32 publications

Gene Expression of Protease Inhibitors in Tomato Plants with Invasion by Root-Knot Nematode Meloidogyne incognita and Modulation of Their Activity with Salicylic and Jasmonic Acids

Gene Expression of Protease Inhibitors in Tomato Plants with Invasion by Root-Knot Nematode Meloidogyne incognita and Modulation of Their Activity with Salicylic and Jasmonic Acids

A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida

Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, Globodera pallida

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