Expression of genes encoding chitin-binding proteins (PR-4) and hevein-like antimicrobial peptides in transgenic tomato plants enhanced resistanse to Phytophthora infestance

Khaliluev, Marat R.; Mamonov, A. G.; Смирнов, А. Н.; Харченко, П. Н.; Dolgov, Sergey

doi:10.3103/s1068367411040082

Cited by 17 publications

(5 citation statements)

References 6 publications

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“…Transgenic plants were produced by Agrobacterium-mediated transformation using binary vector pBI121ac, containing targeting the ac gene under the cauliflower mosaic virus 35S RNA promoter (CaMV35S), the terminator of nopaline synthase (NOS) gene, and the nptII gene for the selection of transgenic plants. These transgenic plants were more resistant to Phytophthora infestans [9,42]. Wild type and transgenic plants were grown in plastic pots filled with soil, at standard agricultural conditions (22-25 • C day-time temperature and 18-19 • C night-time temperature, humidity 60-70%, illumination 2500 lx).…”

Section: Plant Materialsmentioning

confidence: 99%

Distinct Differentiation Characteristics of Endothelium Determine Its Ability to Form Pseudo-Embryos in Tomato Ovules

Chaban

Баранова

Кононенко

et al. 2019

IJMS

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The endothelium is an additional cell layer, differentiating from the inner epidermis of the ovule integument. In tomato (Solanum lycopersicum L.), after fertilization, the endothelium separates from integument and becomes an independent tissue developing next to the growing embryo sac. In the absence of fertilization, the endothelium may proliferate and form pseudo-embryo. However, the course of the reorganization of endothelium into pseudo-embryo in tomato ovules is poorly understood. We aimed to investigate specific features of endothelium differentiation and the role of the endothelium in the development of fertilized and unfertilized tomato ovules. The ovules of tomato plants (“YaLF” line), produced by vegetative growth plants of transgenic tomato line expressing the ac gene, encoding chitin-binding protein from Amaranthus caudatus L., were investigated using light and transmission electron microscopy. We showed that in the fertilized ovule of normally developing fruit and in the unfertilized ovule of parthenocarpic fruit, separation of the endothelium from integument occurs via programmed death of cells of the integumental parenchyma, adjacent to the endothelium. Endothelial cells in normally developing ovules change their structural and functional specialization from meristematic to secretory and back to meristematic, and proliferate until seeds fully mature. The secretory activity of the endothelium is necessary for the lysis of dying cells of the integument and provides the space for the growth of the new sporophyte. However, in ovules of parthenocarpic fruits, pseudo-embryo cells do not change their structural and functional organization and remain meristematic, no zone of lysis is formed, and pseudo-embryo cells undergo programmed cell death. Our data shows the key role of the endothelium as a protective and secretory tissue, needed for the normal development of ovules.

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Section: Plant Materialsmentioning

confidence: 99%

Distinct Differentiation Characteristics of Endothelium Determine Its Ability to Form Pseudo-Embryos in Tomato Ovules

Chaban

Баранова

Кононенко

et al. 2019

IJMS

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“…It exhibited antifungal activity against chitin-containing and chitin-free fungi (F. solani, F. oxysporum, Bipolaris sorokiniana) and antibacterial properties against Gram-negative (e.g., P. syringae, Erwinia carotovora) and Gram-positive bacteria (Clavibacter michiganense), and oomycete Phytophthora infestans [120]. Two genes encoding hevein-like antimicrobial peptides amp1 and amp2 were transformed into the tomato genome to obtain P. infestans-resistant plants [121]. The binary vector pB-AMP2 carrying a full length of SmAMP2 from the chickweed (Stellaria media) was used for genetic transformation of tomato.…”

Section: The Use Of Hevein-like Antimicrobial Peptide Genes In Transg...mentioning

confidence: 99%

Plant-Derived Antimicrobial Peptides: A Plant Defense Weapon against Biotic and Abiotic Stresses

Nguyen,

Ha-Tran,

Tran

et al. 2023

Preprint

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Global agriculture has been forced to increase food production to feed the growing human population, while confronting various environmental obstacles such as global warming, resistance to pathogens, and constraints on arable land caused by soil salinity, drought, rising sea level, saltwater intrusion, and urbanization. Regarding abiotic stresses, salinity is a worldwide problem for agricultural production. Many efforts, therefore, have been made to cope with the environmental challenges, however, the progress of salinization, which is mainly caused and accelerated by anthropogenic activities, is likely faster than our progress in finding ways to deal with this problem. In addition, drought represents a global threat to the production of major crops. In addition, pests and pathogens cause significant crop losses and diminish global food security. Among the various strategies that have been investigated and applied in plant science, antimicrobial peptides derived from plants have caught widespread attention from scientists since these peptides exhibit beneficial biological activities. In agricultural science, there have been reports on the roles of antimicrobial peptides with active properties against biotic and abiotic stresses. Non-specific plant lipid transfer proteins, thionins, systemins, defensins, cyclotides, and heveins-like antimicrobial peptides are common antimicrobial peptides that have been found to be involved in the defense system against fungi and insect pests. Based on their potential ability to protect crops from pests, bacteria, and pathogenic fungi, the use of antimicrobial peptide genes in creating transgenic plants has been largely conducted during the last decades, and these studies have obtained positive results against the growth of fungi and bacteria. This review will focus on the latest progress in studies of antimicrobial peptides related to biotic and abiotic stress tolerance in plants. We will also update the current progress in the development of antimicrobial peptide-based transgenic crops.

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“…Hevein merupakan protein yang dihasilkan oleh beberapa jenis tanaman, (terutama karet) yang memiliki kemampuan antimikroba (Berthelot et al, 2016). Khaliluev et al, (2011)…”

Section: P Nicotianae Merupakan Jamur Dari Keluargaunclassified

PENYAKIT ZEBRA PADA SISAL (AGAVE SPP.): TANTANGAN BAGI PENGEMBANGAN SISAL DI INDONESIA DAN MANAGEMEN PENGENDALIANNYA;Zebra Disease of Sisal: Challenge for Sisal Development in Indonesia and Its Control Management

Yulianti¹,

Wijayanti²,

Setyo-Budi³

2020

Bul. Tan. Tembakau, Serat & Minyak Industri

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Penyakit Zebra merupakan salah satu penyakit penting pada tanaman sisal. Penyakit ini disebabkan oleh jamur Phytophthora nicotianae Breda de Haan. Ada tiga fase infeksi jamur ini pada tanaman sisal, yaitu fase bercak daun zebra, busuk batang (bole rot), dan busuk ujung daun (spike rot). Infeksi pada daun akan menurunkan mutu serat, bahkan pada tingkat kejadian yang parah dapat menyebabkan lembaran daun tidak dapat dipanen dan kematian tanaman. Pengembangan tanaman sisal di Indonesia bagian Timur dengan menggunakan varietas introduksi H11648 yang rentan terhadap penyakit ini perlu diwaspadai dan diantisipasi kejadian dan penyebarannya. Makalah ini mengulas tentang penyakit zebra dan manajemen pengendaliannya secara terpadu, mulai dari upaya pemuliaan, pengendalian hayati, penggunaan ekstrak nabati dan fungisida sintetis. Untuk mendukung pengembangan sisal di NTB dan NTT yang baru dimulai, diperlukan penelitian terutama yang berkaitan dengan pengendalian penyakit ini.

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Expression of genes encoding chitin-binding proteins (PR-4) and hevein-like antimicrobial peptides in transgenic tomato plants enhanced resistanse to Phytophthora infestance

Cited by 17 publications

References 6 publications

Distinct Differentiation Characteristics of Endothelium Determine Its Ability to Form Pseudo-Embryos in Tomato Ovules

Distinct Differentiation Characteristics of Endothelium Determine Its Ability to Form Pseudo-Embryos in Tomato Ovules

Plant-Derived Antimicrobial Peptides: A Plant Defense Weapon against Biotic and Abiotic Stresses

PENYAKIT ZEBRA PADA SISAL (AGAVE SPP.): TANTANGAN BAGI PENGEMBANGAN SISAL DI INDONESIA DAN MANAGEMEN PENGENDALIANNYA;Zebra Disease of Sisal: Challenge for Sisal Development in Indonesia and Its Control Management

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