Expression of genes encoding extracellular matrix proteins: A macroarray study

Futyma, Konrad; Miotła, Paweł; Rozynska, K; Zdunek, Małgorzata; Semczuk, Andrzej; Rechberger, Tomasz; Wojcierowski, Jacek

doi:10.3892/or.2014.3493

Cited by 10 publications

(11 citation statements)

References 44 publications

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“…We observed an increased expression of LAMC1 in higher grades of endometrioid carcinoma as compared to normal endometrium and low-grade endometrioid carcinoma. This finding, together with a previous report demonstrating that other extracellular matrix proteins including aggrecan, vitronectin, tenascin R, nidogen and type VIII (chain α1) collagen and type XI (chain α) collagen are upregulated in endometrioid carcinoma [21], indicates a highly dynamic remodeling of the extracellular matrix during tumor progression. Our results from an in vitro cell motility and invasion assay suggest that expression of the laminin γ1 chain is required to promote cell motility and invasion of endometrioid carcinoma cells in culture.…”

Section: Discussionsupporting

confidence: 73%

Laminin C1 expression by uterine carcinoma cells is associated with tumor progression

Kashima

Wang

et al. 2015

Gynecologic Oncology

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Objectives Molecular markers associated with tumor progression in uterine carcinoma are poorly defined. In this study, we determine whether upregulation of LAMC1, a gene encoding extracellular matrix protein, laminin γ1, is associated with various uterine carcinoma subtypes and stages of tumor progression. Methods An analysis of the immunostaining patterns of laminin γ1 in normal endometrium, atypical hyperplasia, and a total of 150 uterine carcinomas, including low-grade and high-grade endometrioid carcinomas, uterine serous and clear cell carcinoma, was performed. Clinicopathological correlation was performed to determine biological significance. The Cancer Genome Atlas (TCGA) data set was used to validate our results. Results As compared to normal proliferative and secretory endometrium, for which laminin γ1 immunore-activity was almost undetectable, increasing laminin C1 staining intensity was observed in epithelial cells from atypical hyperplasia to low-grade endometrioid to high-grade endometrioid carcinoma, respectively. Laminin γ1 expression was significantly associated with FIGO stage, myometrial invasion, cervical/adnexal involvement, angiolymphatic invasion and lymph node metastasis. Similarly, analysis of the endometrial carcinoma data set from TCGA revealed that LAMC1 transcript levels were higher in high-grade than those in low-grade endometrioid carcinoma. Silencing IAMC1 expression by siRNAs in a high-grade endometrioid carcinoma cell line did not affect its proliferative activity but significantly suppressed cell motility and invasion in vitro. Conclusions These data suggest that laminin γ1 may contribute to the development and progression of uterine carcinoma, likely through enhancing tumor cell motility and invasion. Laminin γ1 warrants further investigation regarding its role as a biomarker and therapeutic target in uterine carcinoma

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Section: Discussionsupporting

confidence: 73%

Laminin C1 expression by uterine carcinoma cells is associated with tumor progression

Kashima

Wang

et al. 2015

Gynecologic Oncology

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“…PSG4 is involved in regulation of innate immune system [57] whereas BRUNOL5 regulates gene transcription through pre-mRNA alternative splicing and mRNA editing [58]. BARD1 is implicated in transcriptional regulation, control of DNA damage repair, and cell cycle [59], whereas ACAN and FXYD6 are important for cell adhesion and cell-cell communication [60,61]. Another interesting finding is that one of the genes containing hypomethylated CpG sites in blood DNA of cases is TET1.…”

Section: (B))mentioning

confidence: 99%

Loci-specific differences in blood DNA methylation in HBV-negative populations at risk for hepatocellular carcinoma development

et al. 2018

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Late onset of clinical symptoms in hepatocellular carcinoma (HCC) results in late diagnosis and poor disease outcome. Approximately 85% of individuals with HCC have underlying liver cirrhosis. However, not all cirrhotic patients develop cancer. Reliable tools that would distinguish cirrhotic patients who will develop cancer from those who will not are urgently needed. We used the Illumina HumanMethylation450 BeadChip microarray to test whether white blood cell DNA, an easily accessible source of DNA, exhibits site-specific changes in DNA methylation in blood of diagnosed HCC patients (post-diagnostic, 24 cases, 24 controls) and in prospectively collected blood specimens of HCC patients who were cancer-free at blood collection (pre-diagnostic, 21 cases, 21 controls). Out of 22 differentially methylated loci selected for validation by pyrosequencing, 19 loci with neighbouring CpG sites (probes) were confirmed in the pre-diagnostic study group and subjected to verification in a prospective cirrhotic cohort (13 cases, 23 controls). We established for the first time 9 probes that could distinguish HBV-negative cirrhotic patients who subsequently developed HCC from those who stayed cancer-free. These probes were identified within regulatory regions of BARD1, MAGEB3, BRUNOL5, FXYD6, TET1, TSPAN5, DPPA5, KIAA1210, and LSP1. Methylation levels within DPPA5, KIAA1210, and LSP1 were higher in prospective samples from HCC cases vs. cirrhotic controls. The remaining probes were hypomethylated in cases compared with controls. Using blood as a minimally invasive material and pyrosequencing as a straightforward quantitative method, the established probes have potential to be developed into a routine clinical test after validation in larger cohorts.

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“…Thus, an important functional implication of NID1 was suggested in the blood-vessel tissue barrier [ 67 ]. Furthermore, NID1 may be involved in the defense against infiltration of cancer cells [ 68 ]. Another highly downregulated protein by IL27 was tropomyosin 1 (TPM1), which is known as a tumour suppressor.…”

Section: Discussionmentioning

confidence: 99%

Role of Calprotectin as a Modulator of the IL27‐Mediated Proinflammatory Effect on Endothelial Cells

Dorosz

Ginolhac

Kähne

et al. 2015

Mediators of Inflammation

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An underlying endothelial dysfunction plays a fundamental role in the pathogenesis of cardiovascular events and is the central feature of atherosclerosis. The protein-based communication between leukocytes and inflamed endothelial cells leading to diapedesis has been largely investigated and several key players such as IL6, TNFα, or the damage associated molecular pattern molecule (DAMP) calprotectin are now well identified. However, regarding cytokine IL27, the controversial current knowledge about its inflammatory role and the involved regulatory elements requires clarification. Therefore, we examined the inflammatory impact of IL27 on primary endothelial cells and the potentially modulatory effect of calprotectin on both transcriptome and proteome levels. A qPCR-based screening demonstrated high IL27-mediated gene expression of IL7, IL15, CXCL10, and CXCL11. Calprotectin time-dependent downregulatory effects were observed on IL27-induced IL15 and CXCL10 gene expression. A mass spectrometry-based approach of IL27 ± calprotectin cell stimulation enlightened a calprotectin modulatory role in the expression of 28 proteins, mostly involved in the mechanism of leukocyte transmigration. Furthermore, we showed evidence for STAT1 involvement in this process. Our findings provide new evidence about the IL27-dependent proinflammatory signaling which may be under the control of calprotectin and highlight the need for further investigations on molecules which might have antiatherosclerotic functions.

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Expression of genes encoding extracellular matrix proteins: A macroarray study

Cited by 10 publications

References 44 publications

Laminin C1 expression by uterine carcinoma cells is associated with tumor progression

Laminin C1 expression by uterine carcinoma cells is associated with tumor progression

Loci-specific differences in blood DNA methylation in HBV-negative populations at risk for hepatocellular carcinoma development

Role of Calprotectin as a Modulator of the IL27‐Mediated Proinflammatory Effect on Endothelial Cells

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