Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization

Mizusawa, Yuri; Kuji, Naoaki; Tanaka, Yasumitsu; Tanaka, Mamoru; Ikeda, Eiji; Komatsu, Setsuko; Kato, Shingo; Yoshimura, Yasunori

doi:10.1016/j.fertnstert.2008.11.028

Cited by 18 publications

(9 citation statements)

References 40 publications

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“…Interestingly, incubation in DREX did not decrease H3K9me2 occupancy at multiple heterochromatic elements, suggesting that DREX affects condensation but not the actual heterochromatin content of somatic nuclei. Oocyte specific H1s might be one of the factors contributing to heterochromatin decondensation since, in humans, H1oo is required for decondensation of sperm chromatin 51 . At long incubation times, HP1a foci reform and extrude from nuclei.…”

Section: Discussionmentioning

confidence: 99%

Chromatin remodeling in Drosophila preblastodermic embryo extract

Šatović

Font-Mateu

Carbonell

et al. 2018

Sci Rep

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Chromatin is known to undergo extensive remodeling during nuclear reprogramming. However, the factors and mechanisms involved in this remodeling are still poorly understood and current experimental approaches to study it are not best suited for molecular and genetic analyses. Here we report on the use of Drosophila preblastodermic embryo extracts (DREX) in chromatin remodeling experiments. Our results show that incubation of somatic nuclei in DREX induces changes in chromatin organization similar to those associated with nuclear reprogramming, such as rapid binding of the germline specific linker histone dBigH1 variant to somatic chromatin, heterochromatin reorganization, changes in the epigenetic state of chromatin, and nuclear lamin disassembly. These results raise the possibility of using the powerful tools of Drosophila genetics for the analysis of chromatin changes associated with this essential process.

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Section: Discussionmentioning

confidence: 99%

Chromatin remodeling in Drosophila preblastodermic embryo extract

Šatović

Font-Mateu

Carbonell

et al. 2018

Sci Rep

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“…On the other hand, in mouse and human embryos, H1oo is replaced by somatic H1s very early during embryogenesis at the 2-4 cells blastula stage before the germ line is determined (Tanaka et al 2001 (Fig. 1) and, later, is expressed in the primordial follicles (Mizusawa et al 2010). …”

Section: Structure Conservation and Expression Patternmentioning

confidence: 99%

Germline-specific H1 variants: the “sexy” linker histones

2015

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“…The presence of the protein was also reported in human oocytes throughout oogenesis, including the primordial follicle stage (Mizusawa et al. ). Yet, we did not detect any expression of H1FOO in oocytes from primordial follicles.…”

Section: Discussionmentioning

confidence: 67%

“…In murine oocytes, transcripts of H1FOO were detected in oocytes from primordial follicles, and the level of transcripts increased in primary and secondary follicles. The presence of the protein was also reported in human oocytes throughout oogenesis, including the primordial follicle stage (Mizusawa et al 2010). Yet, we did not detect any expression of H1FOO in oocytes from primordial follicles.…”

Section: Discussionmentioning

confidence: 70%

Transcription Profile of Candidate Genes for the Acquisition of Competence During Oocyte Growth in Cattle

Bessa

Nishimura

Franco

et al. 2013

Reprod Domestic Animals

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The aim of this study was to investigate the expression profile of candidate genes involved in competence during oocyte growth. The candidate genes (BMP15, OOSP1, H1FOO, H2A, H3A, H4, SLBP, DNMT1, DNMT3B, HAT1, HDAC2 and SUV39H1) were selected because of their possible involvement in determining oocyte developmental competence. Pre-antral and antral follicles were isolated from the ovaries of Zebu (Bos indicus) cows, measured and classified into the following categories according to their diameter: (i) oocytes from primordial follicles: diameter <20 μm, (ii) oocytes from primary follicles: 25-35 μm, (iii) oocytes from small secondary follicles: 40-60 μm, (iv) oocytes from large secondary follicles: 65-85 μm, (v) oocytes from small antral follicles: 100-120 μm, and (vi) oocytes from large antral follicles: >128 μm. Total RNA was extracted from four pools of 25 oocytes for each category of follicles, and the genes were quantified by qPCR. Target gene expression was normalized using the gene PPIA. The results suggest that stocks of the studied transcript genes accumulate before the final phase of folliculogenesis. The HDAC2 gene was the only gene in which a differential expression was observed at stage associated with competence acquisition.

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Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization

Cited by 18 publications

References 40 publications

Chromatin remodeling in Drosophila preblastodermic embryo extract

Chromatin remodeling in Drosophila preblastodermic embryo extract

Germline-specific H1 variants: the “sexy” linker histones

Transcription Profile of Candidate Genes for the Acquisition of Competence During Oocyte Growth in Cattle

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