Expression of microsomal triglyceride transfer protein in lipoprotein-synthesizing tissues of the developing chicken embryo

Eresheim, Christine; Plieschnig, Julia A.; Ivessa, N. Erwin; Schneider, Wolfgang J.; Hermann, Marcela

doi:10.1016/j.biochi.2013.12.020

Cited by 11 publications

(14 citation statements)

References 49 publications

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“…Similarly, in D. melanogaster , MTP transcripts were found in the fat body (an organ analog to vertebrate adipose tissue and liver), and MTP proteins are reported to promote the production of Lpp and large lipid transfer particles ( 14 ). MTP transcripts were also found in vertebrates in tissues expressing apoB, such as liver, intestine, retina ( 45 ), kidney ( 46 ), myocardium ( 47 ), placenta, and yolk sac ( 48 – 50 ). Here, MTP functions as an essential chaperone for the assembly and secretion of apoB-lipoproteins.…”

Section: Discussionmentioning

confidence: 99%

Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis)

Khan

Dalvin

Nilsen

et al. 2017

Journal of Lipid Research

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The salmon louse, Lepeophtheirus salmonis, is an endemic ectoparasite on salmonid fish that is challenging for the salmon farming industry and wild fish. Salmon lice produce high numbers of offspring, necessitating sequestration of large amounts of lipids into growing oocytes as a major energy source for larvae, most probably mediated by lipoproteins. The microsomal triglyceride transfer protein (MTP) is essential for the assembly of lipoproteins. Salmon lice have three L. salmonis MTP (LsMTP) transcript variants encoding two different protein isoforms, which are predicted to contain three β-sheets (N, C, and A) and a central helical domain, similar to MTPs from other species. In adult females, the LsMTPs are differently transcribed in the sub-cuticular tissues, the intestine, the ovary, and in the mature eggs. RNA interference-mediated knockdown of LsMTP in mature females gave offspring with significantly fewer neutral lipids in their yolk and only 10–30% survival. The present study suggests the importance of LsMTP in reproduction and lipid metabolism in adult female L. salmonis, a possible metabolic bottleneck that could be exploited for the development of new anti-parasitic treatment methods.

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Section: Discussionmentioning

confidence: 99%

Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis)

Khan

Dalvin

Nilsen

et al. 2017

Journal of Lipid Research

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“…Our results demonstrated that ACRB expressed higher levels of Apo-B and MTTP on E18 when compared to COBB. MTTP plays an important role in synthesis, translocation and secretion of Apo-B-containing lipoproteins in the cell [29]. This result may be partially explained by the fact that ACRB utilizes more yolk than COBB as described above.…”

Section: Discussionmentioning

confidence: 96%

“…This result may be partially explained by the fact that ACRB utilizes more yolk than COBB as described above. Some evidence showed that Apo-B also highly expressed in the yolk sac during embryonic development [29]. However, we only investigated the gene expression in the liver in this study.…”

Section: Discussionmentioning

confidence: 99%

Fatty acid composition and regulatory gene expression in late-term embryos of ACRB and COBB broilers

Su¹,

Wang

Suh

et al. 2019

Preprint

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Background:Cobb broilers (COBB) have been heavily selected for their production performance in the past several decades, while the Athens Canadian Random Bred (ACRB) chickens, a meat-type breed, has been kept as a non-selected control strain. The purpose of this study was to compare these two lines of chickens at late-embryonic development and identify the molecular markers and fatty acid profiles underlining their differences in growth performance due to selection. Results: COBB had higher egg weight, embryo weight, and breast and fat ratio. The gene expression in the liver showed an interaction between age and breed on FASN (fatty acid synthase) expression with the highest level in COBB at E18. ACRB had higher Apo-B (apolipoprotein B) and MTTP (microsomal triglyceride transfer protein) expression, but lower SREBP-1(regulatory element-binding protein 1) expression compared to COBB. No difference was found in myogenesis gene expression in the muscle between two breeds. For the fatty acid composition, muscle was largely affected by both breed and age. Yolk and liver were affected mainly by breed and age, respectively. Constant interaction effects in docosahexaenoic acid (DHA), indicating the highest level in all the tested tissues of ACRB at E14 and the constant main effects with higher myristic, palmitic and gondoic, but lower linolenic acid in the liver and yolk of COBB compared to in those of ACRB. At last, fat accumulation in the liver had no obvious difference between the breeds but was higher when embryo was older. Conclusions: Broiler breed affects egg, embryo and tissue weight, as well as FA composition in initial egg yolk and throughout the embryonic development. The highest docosahexaenoic percentage was observed in ACRB, indicating that genetic selection may result in fatty acid profile changes in chicken tissues and eggs.

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“…GM3, as a suppressor of cellular TG synthesis and secretion, directly downregulated the level of MTP synthesis. MTP known as a target for the inhibition of ApoB secretion is essential for the hepatic secretion of ApoB‐containing lipoprotein [Ivessa et al, ; Eresheim et al, ; Lee and Hegele, ] by mediating the transfer of TG to nascent ApoB. Because GM3 inhibits ApoB secretion and transcriptional activity of MTP, assembly and secretion of VLDL in liver cell are largely controlled by newly synthesized ApoB.…”

Section: Discussionmentioning

confidence: 99%

Monosialyl Ganglioside GM3 Decreases Apolipoprotein B-100 Secretion in Liver Cells

et al. 2017

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Some sialic acid-containing glycolipids are known to regulate development of atherosclerosis with accumulated plasma apolipoprotein B-100 (Apo-B)-containing lipoproteins, because Apo-B as an atherogenic apolipoprotein is assembled mainly in VLDL and LDL. Previously, we have elucidated that disialyl GD3 promotes the microsomal triglyceride transfer protein (MTP) gene expression and secretion of triglyceride (TG)-assembled ApoB, claiming the GD3 role in ApoB lipoprotein secretion in liver cells. In the synthetic pathway of gangliosides, GD3 is synthesized by addition of a sialic acid residue to GM3. Thus, there should be some regulatory links between GM3 and GD3. In this study, exogenous and endogenous monosialyl GM3 has been examined how GM3 plays a role in ApoB secretion in Chang liver cells in a view point of MTP and ApoB degradation in the same cells. The level of GM3 ganglioside in the GM3 synthase gene-transfected cells was increased in the cell extract, but not in the medium. In addition, GM3 synthase gene-transfected cells showed a diminished secretion of TG-enriched ApoB with a lower content of TG in the medium. Exogenous GM3 treatment for 24 h exerted a dose dependent inhibitory effect on ApoB secretion together with TG, while a liver-specific albumin was unchanged, indicating that GM3 effect is limited to ApoB secretion. GM3 decreased the mRNA level of MTP gene, too. ApoB protein assembly dysregulated by GM3 indicates the impaired ApoB secretion is caused by a proteasome-dependent pathway. Treatment with small interfering RNAs (siRNAs) decreased ApoB secretion, but GM3-specific antibody did not. These results indicate that plasma membrane associated GM3 inhibits ApoB secretion, lowers development of atherosclerosis by decreasing the secretion of TG-enriched ApoB containing lipoproteins, suggesting that GM3 is an inhibitor of ApoB and TG secretion in liver cells. J. Cell. Biochem. 118: 2168-2181, 2017. © 2017 Wiley Periodicals, Inc.

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Expression of microsomal triglyceride transfer protein in lipoprotein-synthesizing tissues of the developing chicken embryo

Cited by 11 publications

References 49 publications

Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis)

Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis)

Fatty acid composition and regulatory gene expression in late-term embryos of ACRB and COBB broilers

Monosialyl Ganglioside GM3 Decreases Apolipoprotein B-100 Secretion in Liver Cells

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