Expression of mRNA encoding insulin-like growth factors I and II by uterine tissues and placenta during pregnancy in the rat

Correia-da-Silva, Georgina; Bell, Steven; Teixeira, Natércia

doi:10.1002/(sici)1098-2795(199907)53:3<294::aid-mrd5>3.0.co;2-0

Cited by 35 publications

(14 citation statements)

References 48 publications

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“…The uterus also expressed high levels of mRNA for IGF-I. Previous studies in pregnant rats (Correia-Da-Silva et al 1999) and pigs (Sterle et al 1998) also showed an expression of mRNA for IGF-I in uterus, although it was not compared with other tissues besides the placenta. Consistent with our results, low levels or no expression of mRNA for IGF-I have been detected in the placenta in guinea pigs (Han et al 1999) and rats (Möller et al 1991).…”

Section: Discussionmentioning

confidence: 85%

Effects of food restriction and pregnancy on the expression of insulin-like growth factors-I and -II in tIssues from guinea pigs

Olausson¹,

Sohlström²

2003

Journal of Endocrinology

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The insulin-like growth factor (IGF) system is subjected to pregnancy-associated changes in the circulation and is suggested to be of importance for partitioning of nutrients between the mother and the foetus. Interestingly, maternal undernutrition alters the pregnancy-associated changes, with possible adverse consequences for the mother and the foetus. However, it is not known how malnutrition and pregnancy alter the expression of mRNA for IGFs locally in different tissues. The aims of this study were to investigate where IGF-I and IGF-II are expressed in guinea pigs and how this expression is altered during food restriction and pregnancy. Ad libitum-fed and foodrestricted (fed 70% of the ad libitum-fed intake four weeks before pregnancy and throughout the study) guinea pigs were mated. On day 40 of pregnancy and on the corresponding day for virginal females the animals were killed. mRNA for IGF-I and IGF-II was analysed in various organs/tissues by solution hybridisation. mRNA for IGF-I was expressed in high amounts in uterus, liver and adipose tissues. The expression was not affected by food restriction, but was increased in liver and adipose tissue and decreased in uterus by pregnancy. mRNA for IGF-II was expressed in high amounts in the placenta and liver. In the placenta the expression was decreased by food restriction. Pregnancy increased the levels of mRNA for IGF-II in the liver. Food-restricted dams had smaller foetuses and placentas. In conclusion, this study indicates an important role for the adipose tissue during gestation, not only as an energy store but also as an endocrine tissue expressing IGF-I. The decreased expression of IGF-II in the placenta due to food restriction is suggested to have adverse effects on placental structure and function.

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Section: Discussionmentioning

confidence: 85%

Effects of food restriction and pregnancy on the expression of insulin-like growth factors-I and -II in tIssues from guinea pigs

Olausson¹,

Sohlström²

2003

Journal of Endocrinology

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“…An important growth factor in placental development is IGF2 (Redline et al, 1993;Correia-da-Silva et al, 1999). Also, Igf 2 is an imprinted gene, and it has been shown recently that imprinting may be disrupted in interspecific Peromyscus hybrids (Vrana et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Proliferation and growth factor expression in abnormally enlarged placentas of mouse interspecific hybrids

Zechner

Hemberger

Constância

et al. 2002

Developmental Dynamics

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It has been shown previously that abnormal placental growth occurs in crosses and backcrosses between different mouse (Mus) species. In such crosses, late gestation placentas may weigh between 13 and 848 mg compared with a mean placental weight of approximately 100 mg in late gestation M. musculus intraspecific crosses. A locus on the X-chromosome was shown to segregate with placental dysplasia. Thus in the (M. musculus ؋ M. spretus)F1 ؋ M. musculus backcross, placental hyperplasia cosegregates with a M. spretus derived X-chromosome. Here we have investigated whether increased cell proliferation and aberrant expression of two genes that are involved in placental growth control, Igf2 and Esx1, may cause, or contribute to placental hyperplasia. Increased bromodeoxyuridine labeling of nuclei, reflecting enhanced proliferation, was indeed observed in hyperplastic placentas when compared with normal littermate placentas. Also, increased expression of Igf 2 was seen in giant cells and spongiotrophoblast. However, when M. musculus ؋ M. spretus F1 females were backcrossed with males that were heterozygous for a targeted mutation of the Igf 2 gene, placentas that carried a M. spretus derived X-chromosome and were negative for a functional Igf 2 allele exhibited an intermediate placental phenotype. Furthermore, in early developmental stages of placental hyperplasia, we observed a decreased expression of the X-chromosomal Esx1 gene. This finding suggests that abnormal expression of both Igf 2 and Esx1 contributes to abnormal placental development in mouse interspecific hybrids. However, Esx1 is not regulated by IGF2.

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“…Growth of the placenta is regulated by several growth factors, including placental growth factor (PlGF), vascular endothelial growth factor (VEGF), and fibroblast growth factor-2 [1, 14, 15]. These growth factors are responsible for fetal development as well as placenta growth [5,9,20,22]. PlGF is a polypeptide growth factor that shares a 53% amino acid sequence homology with the platelet-derived growth factor domain of VEGF [17].…”

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confidence: 99%

Streptozotocin-Induced Diabetes Decreases Placenta Growth Factor (PIGF) Levels in Rat Placenta

Koh

Sung

Won

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. The placenta produces several growth factors, including placenta growth factor (PlGF), which are essential for placenta growth and fetal growth. Diabetic pregnancy induces the abnormal placental growth and fetal development. This study investigated whether diabetes in pregnant rats induces changes in PlGF expression in the placenta. Diabetes was induced by a single intravenous injection of streptozotocin (35 mg/kg body weight) on day 0 of pregnancy, blood and tissue samples were collected on day 20 of pregnancy. In the diabetic group, maternal body weight and fetal weight significantly decreased compared to controls. RT-PCR and Western blot analyses showed that expression of PlGF was significantly decreased in placenta by streptozotocin treatment. Immunohistochemical study showed that the positive signal of PlGF in trophoblast cells was decreased in the diabetic group compared to controls. These findings demonstrate the decline of PlGF in the placenta in diabetic pregnancy. KEY WORDS: diabetes, placenta, PlGF.J. Vet. Med. Sci. 69(9): 877-880, 2007 The placenta is a critical organ for both fetal development and the maintenance of pregnancy. Growth of the placenta is regulated by several growth factors, including placental growth factor (PlGF), vascular endothelial growth factor (VEGF), and fibroblast growth factor-2 [1, 14, 15]. These growth factors are responsible for fetal development as well as placenta growth [5,9,20,22]. PlGF is a polypeptide growth factor that shares a 53% amino acid sequence homology with the platelet-derived growth factor domain of VEGF [17]. Unlike VEGF, abundant expression of PlGF is restricted to the placenta [18]. PlGF was formerly known as a potent angiogenic growth factor capable of inducing the proliferation, migration, and activation of endothelial cells [17]. PlGF plays an important endocrinological and nutritional role, and contributes to the regulation of placental function.Gestational diabetes is one of the most common complications of pregnancy, and is associated with both abnormal placental growth and fetal development [3,16,19]. Severely diabetic pregnancy can induce abortion and prematurity [3,19]. Recently, apoptotic cell death was increased in the placenta of pregnant women with gestational diabetes [21]. PlGF acts as an anti-apoptotic factor for trophoblast in vitro [11]. Therefore, we propose that diabetic pregnancy induces change in PlGF expression and this dysregulation may affect placental development. However, little data is available on the expression of PlGF in the placenta tissue in diabetic pregnancy. Therefore, the present study was performed to provide this information. MATERIALS AND METHODSExperimental animals: Female Sprague-Dawley rats (200-220 g, n=30) were purchased from Samtako Co. (Laboratory Animal Breeding Center, Korea), randomly divided into 2 groups, control group and diabetic group (n=15 per group). Animals were maintained under controlled temperature (25°C) and lighting (14/10 light/dark cycle), and were allowed to have free access...

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Expression of mRNA encoding insulin-like growth factors I and II by uterine tissues and placenta during pregnancy in the rat

Cited by 35 publications

References 48 publications

Effects of food restriction and pregnancy on the expression of insulin-like growth factors-I and -II in tIssues from guinea pigs

Effects of food restriction and pregnancy on the expression of insulin-like growth factors-I and -II in tIssues from guinea pigs

Proliferation and growth factor expression in abnormally enlarged placentas of mouse interspecific hybrids

Streptozotocin-Induced Diabetes Decreases Placenta Growth Factor (PIGF) Levels in Rat Placenta

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