Expression of mt2 and smt-B upon cadmium exposure and cold shock in zebrafish (Danio rerio)

Wu, Su Mei; Zheng, Yu Da; Kuo, Chien‐Hsien

doi:10.1016/j.cbpc.2008.05.007

Cited by 19 publications

(15 citation statements)

References 21 publications

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“…Expression of metallothionein gene (mt2) and similar to metallothionein-B gene (smtB) (Wu et al, 2008) was also determined. Expression of mt2 gene in ZF4-Cd cells was 12.76 times higher than that in ZF4-WT cells.…”

Section: Transcriptional Expression Of Abc Transporter and Metallothimentioning

confidence: 99%

MRP proteins as potential mediators of heavy metal resistance in zebrafish cells

Long

Wang

2011

Comparative Biochemistry and Physiology Part C: Toxicology &

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Section: Transcriptional Expression Of Abc Transporter and Metallothimentioning

confidence: 99%

MRP proteins as potential mediators of heavy metal resistance in zebrafish cells

Long

Wang

2011

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…For example, the mt2 signals appeared upon ionic cadmium (Cd 2? ) exposure and cold shock, but smtB signals were not found under the same treatment conditions by whole-mount in situ hybridization in developmental larvae (Wu et al 2008a). However, the mt2 and smtB mRNA levels were, respectively, increased by 4-and 3.3-fold in the brain of adult fish after cold shock (Wu et al 2015).…”

Section: Introductionmentioning

confidence: 95%

“…Our previous study found that the RT-PCR products of relative expressions of MT mRNA and b-actin of mt2 were downregulated in the brain, and smtB was not induced in gills but can be induced in the brain by Cd 2? on mature zebrafish (Wu et al 2008a). These results raise questions regarding the physiological functions of smtB and mt2 in the brain and gills of adult zebrafish after Cd 2?…”

Section: Introductionmentioning

confidence: 97%

“…On the other hand, it was interesting to find that the smtB mRNA showed a tissue-specific expression, and it indicated that the brain contained 2-to 3-fold more of the smtB mRNA expression as compared to other tissues (e.g., gill, liver, and intestine). However, tissue-specific expressions relating to mt2 mRNA cannot be found in adult zebrafish (Wu et al 2008a). Therefore, the present study was to study the physiological roles of smtB and mt2 in the brain after Cd 2?…”

Section: Introductionmentioning

confidence: 97%

“…In addition, MT may be induced in response to oxidative stress and may function to protect tissues from oxidative damage (Sato and Bremner 1993). In zebrafish (Danio rerio) larvae, the genes mt2 and smtB first appeared in 6 hpf (hours of post-fertilization) and 12 hpf of the embryonic stage, respectively (Wu et al 2008a). Our previous studies found that both genes appeared to have different functions between larvae and adult fish.…”

Section: Introductionmentioning

confidence: 98%

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Anti-oxidative functions of mt2 and smtB mRNA expression in the gills and brain of zebrafish (Danio rerio) upon cadmium exposure

Shu

2016

Fish Physiol Biochem

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There were not any past studies about metallothionein isoforms (smtB and mt2) having anti-oxidative functions on zebrafish after Cd exposure. On the other hand, the anti-oxidative enzymatic factors such as superoxide dismutase (sod), glutathione peroxidase (gpx1a), and catalase (cat) are used as references to investigate whether the smtB and mt2 have anti-oxidative responses on the gills and brain of zebrafish after 1-6 h of 0 and 1.78 μM Cd exposure. The anti-oxidative system such as sod, cat, and gpx1a mRNA expressions demonstrated a cascade response upon Cd-induced oxidative stress in the present study. Interestingly, the smtB mRNA expression levels increased by 3.2- to 6.1-fold, and mt2 raised by 4.1- to 11.3-fold in gills at 1 and 3 h after exposure to Cd, respectively. On the other hand, the smtB mRNA levels increased by 10.6- to 58.6-fold, but mt2 mRNA levels increased by 2.3- to 11.1-fold in brain at 1 and 3 h after exposure to Cd, respectively. In addition, both tissues showed increased apoptosis levels at 3 h, and recovery after 6 h of Cd exposure. From the results, we suggest that both mt2 and smtB play a role in anti-oxidation responses within 6 h after exposure to Cd. In conclusion, the smtB mRNA levels have a higher response than mt2 in the brain, but both mRNA expressions appear to have a similar pattern in the gill. We suggest that smtB plays an important role to defend oxidative stress in the brain of adult zebrafish upon acute Cd exposure.

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Metallothioneins’ Responses on Impact of Metal-Based Nanomaterials for Biomedical Use

Stoliar

Stoika

2021

Biomedical Nanomaterials

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Expression of mt2 and smt-B upon cadmium exposure and cold shock in zebrafish (Danio rerio)

Cited by 19 publications

References 21 publications

MRP proteins as potential mediators of heavy metal resistance in zebrafish cells

MRP proteins as potential mediators of heavy metal resistance in zebrafish cells

Anti-oxidative functions of mt2 and smtB mRNA expression in the gills and brain of zebrafish (Danio rerio) upon cadmium exposure

Metallothioneins’ Responses on Impact of Metal-Based Nanomaterials for Biomedical Use

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