Expression of Mycoplasma bovis variable surface membrane proteins in the respiratory tract of calves after experimental infection with a clonal variant of Mycoplasma bovis type strain PG45

Development of a Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay for Diagnosis of Mycoplasma bovis Infection in Cattle

“…Use of a single, highly immunoreactive protein is more likely to result in a specific ELISA. The most immunoreactive antigens in the M. bovis cell membrane are the Vsps (variable surface proteins), but, while the Vsps may have diagnostic utility (32), they are subject to phase and antigenic variation, which may result in variations in the antibody response against them in individual infected cattle and compromise the sensitivity of the assay (33). Therefore, use of a protein such as MilA that appears not to be subject to phase or antigenic variation, but is still highly immunoreactive, is more likely to result in a useful diagnostic tool.…”

Loss of diversity within Mycoplasma bovis isolates collected in France from bovines with respiratory diseases over the last 35 years

“…However DNA rearrangements were shown to occur within the vsp loci in clonal populations (Buchenau et al, 2010;Lysnyansky et al, 1996) so their use for subtyping strains with an epidemiological concordance was limited. In contrast the overall protein patterns obtained by MatrixAssisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) were shown to be promising for subtyping strains of M. bovis (Pereyre et al, 2013).…”

Status of the development of a vaccine against Mycoplasma bovis

“…The variable-surface proteins (Vsps) and other surface proteins have been associated with attachment to host cells [7,8]. One complicating factor is that the capacity of Vsps for phase and antigenic variation [9]. The host-immune responses to M. bovis after natural or experimental infection provide important information that may help in designing a successful vaccine.…”