Expression of neural markers on bone marrow–derived canine mesenchymal stem cells

Kamishina, Hiroaki; Deng, Jie; Oji, Takashi; Cheeseman, Jennifer A.; Clemmons, Roger M.

doi:10.2460/ajvr.67.11.1921

Cited by 50 publications

(63 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, adherent stromal cells isolated from the bone marrow and adipose tissue were used as cBMMSCs and cADMSCs [10,29]. A previous study by Zuk et al demonstrated that plastic-adherent cells isolated from human adipose tissue, labeled as MSCs, also contained non-MSC population [40].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A Comparison of Neurosphere Differentiation Potential of Canine Bone Marrow-Derived Mesenchymal Stem Cells and Adipose-Derived Mesenchymal Stem Cells

et al. 2013

View full text Add to dashboard Cite

ABSTRACT. Stem cell transplantation is one of the most promising yet enigmatic treatments for spinal cord injury (SCI), a common problem in dogs. As pre-differentiated mesenchymal stem cells (MSCs) can be expanded and differentiated into neurospheres in vitro, before being transplanted back, they may prove to be more beneficial for treating SCI. Therefore, we compared the endogenous differentiation potential, including the neuronal cell differentiation, of neurospheres from canine bone marrow MSCs (cBMMSCs) with that of the adipose tissuederived MSCs (cADMSCs). Nestin-positive neurospheres were generated from MSCs derived from the bone marrow and adipose tissue. Neuronal cells were differentiated from the neurospheres derived from both these tissues. Gene expression analysis revealed that Nestin, βIII-tubulin, NCAM, OCT4 and SOX2 were expressed in MSCs and the corresponding neurospheres. Notably, cBMMSC-derived neuronal cells expressed higher levels of βIII-tubulin. The mRNA expressions of NANOG, Nestin, OCT4 and SOX2 were upregulated in neurospheres derived from both. Immunofluorescence analysis detected the expression of neuronal markers, namely, βIII-tubulin, GFAP, S100, NF200 and MAP2, in differentiated neuron-like cells. Our findings highlight that both cBMMSCs and cADMSCs could be differentiated into neurospheres and neuron-like cells, and therefore, these cells are suitable candidates for cell transplantation. Further, cADMSCs form a more suitable cell source, as larger number of cells could be harvested from cADMSC-derived neurospheres. Future studies employing in vivo transplantation models to investigate the effectiveness of MSCs for treating SCI are warranted. KEY WORDS: adipose-derived mesenchymal stem cell, bone marrow-derived mesenchymal stem cell, canine, neurosphere, spinal cord injury.

show abstract

Section: Discussionmentioning

confidence: 99%

“…cBMMSCs were isolated and cultured according to a previous method with minor modifications [10]. Briefly, the bone marrow collected was dissociated, and then resuspended with a pipette.…”

Section: Animalsmentioning

confidence: 99%

A Comparison of Neurosphere Differentiation Potential of Canine Bone Marrow-Derived Mesenchymal Stem Cells and Adipose-Derived Mesenchymal Stem Cells

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In order to assess the maturation of differentiated cells, III-tubulin and NeuN were used as early and mature neuronal markers, respectively. The cross-reactivity of these primary antibodies to canine cells has been confirmed by our preliminary experiment and previous study [7]. Brain slices were washed three times in PBS and permeabilized in 0.4% Triton X-100 in PBS for 30 min at 25C.…”

Section: Preparation Of Canine Bmscs and Fibroblastsmentioning

confidence: 92%

“…Mononucleated cells were isolated on a Ficoll (Ficoll-Paque, StemCell Technologies, Vancouver, Canada) density gradient, washed in PBS, and suspended in culture medium consisting of DMEM (1g/l glucose) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 U/ml penicillin G, 100 g/ml streptomycin sulfate, and 0.25 g/ml amphotericin B). Cell surface marker profiles, growth kinetics, and differentiation properties of isolated canine BMSCs have been described in our previous studies [6][7][8]. Canine skinderived third passage fibroblasts (Coriell Institute for Medical Research) were used for comparative purposes.…”

Section: Preparation Of Canine Bmscs and Fibroblastsmentioning

confidence: 99%

Migration and Differentiation of Canine Bone Marrow Stromal Cells Transplanted into the Developing Mouse Brain

Kamishina

Cheeseman

Farese

et al. 2010

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. To evaluate whether canine bone marrow stromal cells (BMSCs) can migrate and adopt neural phenotypes in the developing mouse brain we transplanted fluorescently labeled BMSCs into the lateral ventricle of immunocompromised neonatal mice. Most fibroblasts, used as a control, and BMSCs isolated from adult dogs remained around the injection site and exhibited a spindle-shaped appearance. A small number of BMSCs from young dogs were found in the subventricular zone, rostral migratory stream, and olfactory bulbs, and retained expression of neuron marker. Our findings suggest that BMSCs isolated from adult dogs have limited ability of migration and differentiation toward neural cells in the developing brain. Bone marrow of young dogs may contain a primitive stem cell population with neural differentiation capacity.

show abstract

“…33 Another study for canine BMCs has shown that mesenchymal stem cells (MSCs) were obtained after canine BMMNC culture with DMEM medium supplemented with 20% FBS and the cell surface marker expression of these cells were comparable to those reported in human and rodent MSCs. 34 Analysis by flow cytometry revealed that these cells were positive for CD90 and MHC-I and negative for CD34 and CD45, indicating that cells with hematopoietic origin were excluded during cell expansion process under this culture condition. 34 BMCs represent an alternative cell source for tissue engineering of vascular grafts.…”

Section: Discussionmentioning

confidence: 99%

Tissue‐Engineered Blood Vessels With Endothelial Nitric Oxide Synthase Activity

et al. 2008

View full text Add to dashboard Cite

Nondegradable synthetic polymer vascular grafts used in cardiovascular surgery have shown serious shortcomings, including thrombosis, calcification, infection, and lack of growth potential. Tissue engineering of vascular grafts with autologous stem cells and biodegradable polymeric materials could solve these problems. The present study is aimed to develop a tissue-engineered vascular graft (TEVG) with functional endothelium using autologous bone marrow-derived cells (BMCs) and a hybrid biodegradable polymer scaffold. Hybrid biodegradable polymer scaffolds were fabricated from poly(lactide-co-e-caprolactone) (PLCL) copolymer reinforced with poly(glycolic acid) (PGA) fibers. Canine bone marrow mononuclear cells were induced in vitro to differentiate into vascular smooth muscle cells and endothelial cells. TEVGs (internal diameter: 10 mm, length: 40 mm) were fabricated by seeding vascular cells differentiated from BMCs onto PGA/PLCL scaffolds and implanted into the abdominal aorta of bone marrow donor dogs (n 5 7). Eight weeks after implantation of the TEVGs, the vascular grafts remained patent. Histological and immunohistochemical analyses of the vascular grafts retrieved at 8 weeks revealed the regeneration of endothelium and smooth muscle and the presence of collagen. Western blot analysis showed that endothelial nitric oxide synthase (eNOS) was expressed in TEVGs comparable to native abdominal aortas. This study demonstrates that vascular grafts with significant eNOS activity can be tissueengineered with autologous BMCs and hybrid biodegradable polymer scaffolds. '

show abstract

Expression of neural markers on bone marrow–derived canine mesenchymal stem cells

Cited by 50 publications

References 52 publications

A Comparison of Neurosphere Differentiation Potential of Canine Bone Marrow-Derived Mesenchymal Stem Cells and Adipose-Derived Mesenchymal Stem Cells

A Comparison of Neurosphere Differentiation Potential of Canine Bone Marrow-Derived Mesenchymal Stem Cells and Adipose-Derived Mesenchymal Stem Cells

Migration and Differentiation of Canine Bone Marrow Stromal Cells Transplanted into the Developing Mouse Brain

Tissue‐Engineered Blood Vessels With Endothelial Nitric Oxide Synthase Activity

Contact Info

Product

Resources

About