Expression of Novel CD44st and MMP2 in NSCLC Tissues and Their Clinical Significance

Abstract

Background: Abnormal expression of some CD44 molecules in tumor tissues can induce the degradation of the extracellular matrix, and is closely associated with axillary lymph node metastasis, angiogenesis, cancer progression, and drug resistance. Patients and Methods: We measured and confirmed the expression of CD44st and MMP2 mRNAs and proteins in the cancer tissues and adjacent normal tissues of postoperative non-small cell lung cancer (NSCLC) patients with either adenocarcinoma (n = 72) or squamous cell carcinoma (n = 53) using quantitative reverse transcription polymerase chain reaction, gene sequencing, and immunohistochemical analysis. Results: CD44st and MMP2 expression were closely associated with the histopathological classification, lymph node metastasis, and TNM stage of the tumors, and the difference was statistically significant (p < 0.05). The median overall survival (OS) for the high CD44st expression group was 30.52 months (95% confidence interval (CI) 24.02-36.15); for the low expression group it was 43.23 months (95% CI 31.81-52.02) (p = 0.020). The median OS for the high MMP-2 expression group was 30.53 months (95% CI 26.69-33.31); for the low expression group it was 40.06 months (95% CI 33.55-46.45) (p = 0.022). Conclusion: The rates of CD44st and MMP2 expression were higher in squamous cell carcinomas than in adenocarcinomas, were closely associated with lymph node metastasis and TNM stage, and affected patients' prognoses.

Correlation between MMP2 expression in lung cancer tissues and clinical parameters: a retrospective clinical analysis

“…We found that the MMP2 was highly expressed in lung cancer tissues but not in adjacent normal tissues, suggesting that high expression of MMP2 might potentially related to lung cancer occurrence and development. Our findings are consistent with previous reports that MMP2 is highly expressed in lung cancer tissues and lowly expressed in normal tissues [ 21 , 22 ].…”

“…In our study, these two factors were implicated into the regression equation and the regression equation was as H(t) = [h 0 (t)] e (2.01X8 + 1.49X9) , which suggested that determining the expression of MMP2 in lung cancer tissues is helpful for predicting the prognosis of lung cancer patients. Our conclusion is similar to previous studies that the high expression of MMP2 in NSCLC was associated with tumor type and clinical stage and has a predictive value for low survival rates, short overall survival (OS) and disease free survival (DFS) [ 21 , 24 , 25 ]. Increased MMP2 correlates with histo-differentiation, lymphatic metastasis, progression, and prognosis of lung cancer, suggesting that it could reflect the situation of progression and migration of lung cancer to a certain extent and that it could be a useful diagnostic, prognostic, and predictive biomarker of lung cancer.…”

“…Increased MMP2 correlates with histo-differentiation, lymphatic metastasis, progression, and prognosis of lung cancer, suggesting that it could reflect the situation of progression and migration of lung cancer to a certain extent and that it could be a useful diagnostic, prognostic, and predictive biomarker of lung cancer. A previous study suggests that the expression of MMP2 in lung squamous cell carcinoma is higher than that in lung adenocarcinoma, and its high expression is closely related to lymph node metastasis and TNM staging, and affects the prognosis of patients [ 21 ]. However, another study suggests that MMP2 protein has nothing to do with the histology of NSCLC, but its level correlates with poorly differentiated tissue and distant metastasis [ 22 ].…”

Increased MMP2 contributes to the diagnosis and prognosis of lung cancer

“…In tissues level, we found that the MMP2 was highly expressed in lung cancer tissues but not in adjacent normal tissues, which suggested that high expression of MMP2 might potentially promote lung cancer occurrence and development. Previous studies have demonstrated that MMP2 is highly expressed in lung cancer tissues and lowly expressed in normal tissues [23,24]. It is well known that the degree of differentiation of lung cancer cells is an independent factor affecting the prognosis of lung cancer patients, which is mainly based on that poorly differentiated lung cancer cells have a high degree of malignancy, and will soon be transferred and relapsed.…”

Soluble Triggering Receptor Expressed on Myeloid Cells 1 in lung cancer

“…Moreover, we found higher sTREM-1 levels in NSCLC than in SCLC, an entity which is considered to be more aggressive than NSCLC. There are few comparisons of MMPs in SCLC and NSCLC in literature, but MMP-2 seems to be more often expressed on NSCLC cells than on SCLC cells 31 , 32 . Regarding MMP-9, higher activity in tumor tissues is reported in NSCLC than in SCLC 33 .…”