Expression of recombinant human IFN-γ protein in soybean (Glycine max L.)

Mehrizadeh, Vahid; Dorani, Ebrahim; Mohammadi, Seyed Abolghasem; Ghareyazie, Behzad

doi:10.1007/s11240-021-02052-z

Cited by 4 publications

(8 citation statements)

References 33 publications

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“…The bioactivity assay confirmed the biological activity (like antiviral) of recombinant hIFN-γ expressed into soyabean (Mehrizadeh et al, 2021).…”

Section: Cereals and Legumessupporting

confidence: 59%

“…The high protein content and easy cultivation under greenhouse conditions make Glycine max (L) a suitable candidate for the expression of different antigens (Cunha et al, 2011;Powell et al, 2011). Mehrizadeh et al (2021) introduced human interferon-γ (hIFN-γ) in soybean as these proteins are of therapeutic importance against viruses. A vector named pCAMBIA1304hIFN-γ is constructed by the research through the ligation of hIFN-γ with pCAMBIA1304 (a plant expression vector) that is transferred via A. tumefaciens for successful transformation.…”

Section: Cereals and Legumesmentioning

confidence: 99%

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Insight of oral vaccines as an alternative approach to health and disease management: An innovative intuition and challenges

Debnath

Thakur

Khushboo

et al. 2021

Biotech & Bioengineering

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Vaccination is the most suitable and persuasive healthcare program for the prohibition of various deadly diseases. However, the higher production cost and purification strategies are out of reach for the developing nations. In this scenario, development of edible vaccine turns out to be the most promising alternative for remodeling the pharmaceutical industry with reduced production and purification costs. Generally, oral route of vaccination is mostly preferred due to its safety, compliance, low manufacturing cost and most importantly the ability to induce immunity in both systemic and mucosal sites. Genetically modified microorganisms and plants could efficiently be used as vehicles for edible vaccines. Edible vaccines are supposed to reduce the risk associated with traditional vaccines. Currently, oral vaccines are available in the market for several viral and bacterial diseases like cholera, hepatitis B, malaria, rabies etc. Herein, the review focuses on the breakthrough events in the area of edible vaccines associated with dietary microbes and plants for better control over diseases.

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“…The bioactivity assay confirmed the biological activity (like antiviral) of recombinant hIFN-γ expressed into soyabean (Mehrizadeh et al, 2021).…”

Section: Cereals and Legumessupporting

confidence: 59%

Section: Cereals and Legumesmentioning

confidence: 99%

Insight of oral vaccines as an alternative approach to health and disease management: An innovative intuition and challenges

Debnath

Thakur

Khushboo

et al. 2021

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…In 1986 and 1989, transgenic plants produced human growth hormone, the first recombinant pharmaceutical protein, and the first recombinant antibody, respectively [ 34 , 35 ]. Green plants and/or plant cell culture-based systems can be employed as large-scale biofactories for the manufacture of recombinant proteins [ 17 , 36 , 37 ]. Raphanus sativus L., an edible plant from the Cruciferae family, is an annual vegetable.…”

Section: Resultsmentioning

confidence: 99%

“…Interferons are a family of cytokines that play important roles in antiviral and antibacterial defenses, immune system activation, and cell growth control [ 17 ]. IFN is a protein that is mostly utilized to treat viral infections and malignant neoplasms.…”

Section: Introductionmentioning

confidence: 99%

“…In 2014, the global interferon trade reached over four billion dollars [ 19 ]. Because of the high cost and great demand for interferon, researchers are focusing their efforts on developing cost-effective and safe protein production technologies [ 17 ]. The objective of the current study is to generate and assess the biological activity of a recombinant human interferon-α2a produced from Raphanus sativus L. plants.…”

Section: Introductionmentioning

confidence: 99%

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A Biotechnological Approach for the Production of Pharmaceutically Active Human Interferon-α from Raphanus sativus L. Plants

et al. 2022

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Human interferon (IFN) is a type of cytokine that regulates the immune system’s response to viral and bacterial infections. Recombinant IFN-α has been approved for use in the treatment of a variety of viral infections as well as an anticancer medication for various forms of leukemia. The objective of the current study is to produce a functionally active recombinant human IFN-α2a from transgenic Raphanus sativus L. plants. Therefore, a binary plant expression construct containing the IFN-α2a gene coding sequence, under the regulation of the cauliflower mosaic virus 35SS promoter, was established. Agrobacterium-mediated floral dip transformation was used to introduce the IFN-α2a expression cassette into the nuclear genome of red and white rooted Raphanus sativus L. plants. From each genotype, three independent transgenic lines were established. The anticancer and antiviral activities of the partially purified recombinant IFN-α2a proteins were examined. The isolated IFN-α2a has been demonstrated to inhibit the spread of the Vesicular Stomatitis Virus (VSV). In addition, cytotoxicity and cell apoptosis assays against Hep-G2 cells (Human Hepatocellular Carcinoma) show the efficacy of the generated IFN-α2a as an anticancer agent. In comparison to bacterial, yeast, and animal cell culture systems, the overall observed results demonstrated the efficacy of using Raphanus sativus L. plants as a safe, cost-effective, and easy-to-use expression system for generating active human IFN-α2a.

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Progress in Soybean Genetic Transformation Over the Last Decade

Guo

Qiu

et al. 2022

Front. Plant Sci.

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Soybean is one of the important food, feed, and biofuel crops in the world. Soybean genome modification by genetic transformation has been carried out for trait improvement for more than 4 decades. However, compared to other major crops such as rice, soybean is still recalcitrant to genetic transformation, and transgenic soybean production has been hampered by limitations such as low transformation efficiency and genotype specificity, and prolonged and tedious protocols. The primary goal in soybean transformation over the last decade is to achieve high efficiency and genotype flexibility. Soybean transformation has been improved by modifying tissue culture conditions such as selection of explant types, adjustment of culture medium components and choice of selection reagents, as well as better understanding the transformation mechanisms of specific approaches such as Agrobacterium infection. Transgenesis-based breeding of soybean varieties with new traits is now possible by development of improved protocols. In this review, we summarize the developments in soybean genetic transformation to date, especially focusing on the progress made using Agrobacterium-mediated methods and biolistic methods over the past decade. We also discuss current challenges and future directions.

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Expression of recombinant human IFN-γ protein in soybean (Glycine max L.)

Cited by 4 publications

References 33 publications

Insight of oral vaccines as an alternative approach to health and disease management: An innovative intuition and challenges

Insight of oral vaccines as an alternative approach to health and disease management: An innovative intuition and challenges

A Biotechnological Approach for the Production of Pharmaceutically Active Human Interferon-α from Raphanus sativus L. Plants

Progress in Soybean Genetic Transformation Over the Last Decade

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