Expression of Recombinant Leptospiral Surface Lipoprotein-Lsa27 in E. coli and Its Evaluation for Serodiagnosis of Bovine Leptospirosis by Latex Agglutination Test

Evaluation of the diagnostic potential of recombinant leptospiral OMP A‐like protein (Loa22) and transmembrane (OmpL37) protein in latex agglutination test for serodiagnosis of leptospirosis in animals

“…As the test can be used as a screening test for the detection of anti‐leptospiral antibodies, if the test result is positive, it needs to be rechecked with another confirmatory test as reported earlier (Alamuri et al . 2020). Since rLoa22 LAT is being simple to perform, it gives the advantages of screening a large scale of samples rapidly, and only the positive samples need to be rechecked with the gold standard test or its equivalent tests for confirmation.…”

“…2015; Alamuri et al . 2020). The harvested induced/uninduced recombinant cultures, mock vector/host bacterial sample lysate and eluted purified fractions were analysed in 12% SDS‐PAGE, and the proteins were transferred on to a nitrocellulose membrane (Sigma‐Aldrich, St. Louis, MO, USA) for immunoblot analysis as per the described method (Nagalingam et al .…”

“…Briefly, 10 μl of rLoa22 or rOmpL37 latex beads was placed in the agglutination card and added 10 μl of test serum to the beads and mixed thoroughly, and the results were recorded within 3 min as 1+, 2+ and 3+++ based on the agglutination reaction as described earlier (Alamuri et al . 2020).…”

“…2015; Alamuri et al . 2020). Further, some of the conserved OMPs are expressed only by pathogenic leptospires during infection and are involved in pathogenesis at both acute and convalescent stages of infection.…”

“…Further, the virulence factors of pathogenic Leptospira expressed in the host cells during the pathogenesis/infection are expected to elicit specific immune responses and may serve as specific diagnostic markers (Ghosh et al 2018). Considering the importance of early detection of leptospirosis by simple and rapid test, an attempt was made to develop rapid field diagnostics as a preliminary screening test using recombinant OMP (LigB and Lsa27 proteins) for the detection of anti-leptospiral antibodies in the sera of bovine (Nagalingam et al 2015;Alamuri et al 2020). Further, some of the conserved OMPs are expressed only by pathogenic leptospires during infection and are involved in pathogenesis at both acute and convalescent stages of infection.…”