Expression of Regucalcin, a calcium-binding protein is regulated by hypoxia-inducible factor-1α

Singh, Tripti; Banerjee, Pallabi; Uditi, None; Kumari, Sarita; Chopra, Anita; Singh, Nagendra; Qamar, Imteyaz

doi:10.1016/j.lfs.2021.120278

Cited by 7 publications

(3 citation statements)

References 28 publications

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“…In terms of mechanism, RGN is in a pathway that may be enhanced or otherwise regulated in LUSC. A study has revealed that HIF-1α protein binds directly to the HRE binding motifs within the RGN promoter to regulate the the expression of RGN 39 . The expression of HIF-1α was significantly higher in LUSC than those in normal lung tissues and related to worse survival time 40 , 41 .…”

Section: Discussionmentioning

confidence: 99%

RGN as a prognostic biomarker with immune infiltration and ceRNA in lung squamous cell carcinoma

Yang

Cheng

Mou

et al. 2023

Sci Rep

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Regucalcin (RGN) is a potent inhibitory protein of calcium signaling and expresses in various tissues. However, the role of RGN in the tumor immunological microenvironment in lung squamous cell carcinoma (LUSC) remains unclear. This study identified the expression of RGN from public databases and immunohistochemistry with clinical specimen. The association between RGN and the tumor immune microenvironment (TIME) was investigated in LUSC by ESTIMATE and CIBERSORT algorithms. Similarly, the Tumor IMmune Estimation Resource (TIMER) database was used to identify the correlation between RGN and immune cells. The ceRNA network was established based on the data obtained from public databases. Finally, prediction of drug response to chemotherapy and immunotherapy was performed to evaluate clinical significance. This study found that RGN expression was significantly downregulated in tumor tissues and closely related to clinical factors and prognosis of LUSC patients. Differentially expressed genes (DEGs) grouped by the expression of RGN were mostly involved in immunobiological processes such as humoral immune response and leukocyte mediated immunity. RGN and its related miRNA (has-miR-203a-3p) and lncRNAs (ZNF876P and PSMG3-AS1) constructed the novel prognosis-related ceRNA network. Plasma cells, T cells CD4 memory resting, Macrophages M0, Macrophages M1, Mast cells resting, Mast cells activated and Neutrophils showed significantly different levels of infiltration between high and low RGN expression groups. The TIMER database showed that RGN expression was positively correlated with certain immune infiltrating cells. High RGN expression group showed a higher TIDE score, a higher dysfunction score and a lower MSI score, presenting a possible lower efficacy after accepting the immunotherapy than low RGN expression group. RGN expression was closely associated with prognosis of LUSC patients and played an important role in tumor microenvironment. This suggests that RGN could be a promising biomarker for assessing immunotherapy efficacy and prognosis.

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Section: Discussionmentioning

confidence: 99%

RGN as a prognostic biomarker with immune infiltration and ceRNA in lung squamous cell carcinoma

Yang

Cheng

Mou

et al. 2023

Sci Rep

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“…HNF4A , as a transcription factor, functions in the maintenance of the circadian rhythm of liver genes [ 50 ]. RGN can modulate Ca 2+ -dependent enzyme activities and is essential for the maintenance of calcium homeostasis [ 51 ]. TIM can determine the formation of circadian rhythm together with period circadian protein [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis on the Regulatory Mechanism of Thoracic Ganglia in Eriocheir sinensis at Post-Molt and Inter-Molt Stages

Wang

Zhou

et al. 2022

Life

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Eriocheir sinensis is an aquatic species found distributed worldwide. It is found in the Yangtze River of China, where the commercial fishing of this valuable catadromous aquatic species has been banned. As an important member of the phylum Arthropoda, E. sinensis grows by molting over its whole lifespan. The central nervous system of Eriocheir sinensis plays an important regulatory role in molting growth. Nevertheless, there are no reports on the regulatory mechanisms of the nervous system in E. sinensis during the molting cycle. In this study, a comparative transcriptome analysis of E. sinensis thoracic ganglia at post-molt and inter-molt stages was carried out for the first time to reveal the key regulatory pathways and functional genes operating at the post-molt stage. The results indicate that pathways and regulatory genes related to carapace development, tissue regeneration, glycolysis and lipolysis and immune and anti-stress responses were significantly differentially expressed at the post-molt stage. The results of this study lay a theoretical foundation for research on the regulatory network of the E. sinensis nervous system during the post-molt developmental period. Detailed knowledge of the regulatory network involved in E. sinensis molting can be used as a basis for breeding improved E. sinensis species, recovery of the wild E. sinensis population and prosperous development of the E. sinensis artificial breeding industry.

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“…The promoter region of the gene has been thoroughly examined [11]. The regucalcin geneʹs expression is enhanced by various transcription factors, such as calcium, AP-1, NF1-A1, RGPR-p117, β-catenin, HIF-1α, NF-κB, STAT3, SMADs, and the aryl hydrocarbon receptor (AHR) [15][16][17][18][19]. These factors have been identified as enhancers.…”

Section: Introductionmentioning

confidence: 99%

Regucalcin Is a Potential Regulator in Human Cancer: Aiming to Expand into Cancer Therapy

Yamaguchi

2023

Cancers

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Regucalcin, a calcium-binding protein lacking the EF-hand motif, was initially discovered in 1978. Its name is indicative of its function in calcium signaling regulation. The rgn gene encodes for regucalcin and is situated on the X chromosome in both humans and vertebrates. Regucalcin regulates pivotal enzymes involved in signal transduction and has an inhibitory function, which includes protein kinases, protein phosphatases, cysteinyl protease, nitric oxide dynthetase, aminoacyl-transfer ribonucleic acid (tRNA) synthetase, and protein synthesis. This cytoplasmic protein is transported to the nucleus where it regulates deoxyribonucleic acid and RNA synthesis as well as gene expression. Overexpression of regucalcin inhibits proliferation in both normal and cancer cells in vitro, independent of apoptosis. During liver regeneration in vivo, endogenous regucalcin suppresses cell growth when overexpressed. Regucalcin mRNA and protein expressions are significantly downregulated in tumor tissues of patients with various types of cancers. Patients exhibiting upregulated regucalcin in tumor tissue have shown prolonged survival. The decrease of regucalcin expression is linked to the advancement of cancer. Overexpression of regucalcin carries the potential for preventing and treating carcinogenesis. Additionally, extracellular regucalcin has displayed control over various types of human cancer cells. Regucalcin may hold a prominent role as a regulatory factor in cancer development. Supplying the regucalcin gene could prove to be a valuable asset in cancer treatment. The therapeutic value of regucalcin suggests its potential significance in treating cancer patients. This review delves into the most recent research on the regulatory role of regucalcin in human cancer development, providing a novel approach for treatment.

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Expression of Regucalcin, a calcium-binding protein is regulated by hypoxia-inducible factor-1α

Cited by 7 publications

References 28 publications

RGN as a prognostic biomarker with immune infiltration and ceRNA in lung squamous cell carcinoma

RGN as a prognostic biomarker with immune infiltration and ceRNA in lung squamous cell carcinoma

Comparative Transcriptome Analysis on the Regulatory Mechanism of Thoracic Ganglia in Eriocheir sinensis at Post-Molt and Inter-Molt Stages

Regucalcin Is a Potential Regulator in Human Cancer: Aiming to Expand into Cancer Therapy

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