Expression of Ring Box-1 protein and its relationship with Fuhrman grade and other clinical-pathological parameters in renal cell cancer

Altintas, Emre; Kaynar, Mehmet; Çelik, Zeliha Esin; Çeli̇k, Murat; Kılıç, Özcan; Akand, Murat; Göktaş, Serdar

doi:10.1016/j.urolonc.2019.09.019

Cited by 5 publications

(6 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sun Y et al has demonstrated that inactivation of either RBX1 or RBX2 inhibits carcinogenesis via various mechanisms, including apoptosis and senescence (17,41,42). However, two other studies found that only RBX2 overexpression was correlated with the poor prognosis in lung cancer (21); as well as high RBX1 expression was related to poor survival only in KIRC patients and high RBX2 expression had a close relation with poor prognosis in all three types of RCC (22,43). At present, the comparison of Ring finger family in the same cancer is rare.…”

Section: Discussionmentioning

confidence: 99%

An integrative pan-cancer analysis revealing the difference in small ring finger family of SCF E3 ubiquitin ligases

Huang

Li²,

Liu³

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

BackgroundThe SCF (Skp1-cullin-F-box proteins) complex is the largest family of E3 ubiquitin ligases that mediate multiple specific substrate proteins degradation. Two ring-finger family members RBX1/ROC1 and RBX2/RNF7/SAG are small molecular proteins necessary for ubiquitin ligation activity of the multimeric SCF complex. Accumulating evidence indicated the involvement of RBX proteins in the pathogenesis and development of cancers, but no research using pan-cancer analysis for evaluating their difference has been directed previously.MethodsWe investigated RBX1/2 expression patterns and the association with clinicopathological features, and survivals of cancer patients obtained from the TCGA pan-cancer data. The binding energies of RBX1/2-CUL1 complexes were preliminarily calculated by using molecular dynamics simulations. Meanwhile, we assessed their immune infiltration level across numerous databases, including TISIDB and Timer database.ResultsHigh expression levels of RBX1/2 were observed in most cancer types and correlated with poor prognosis of patients analyzed. Nonetheless, exceptions were observed: RBX2 expression in KICH was higher than normal renal tissues and played a detrimental role in KICH. The expression of RBX1 was not associated with the prognostic risk of KICH. Moreover, the combination of RBX1 and CUL1 expression is more stable than that of RBX2 and CUL1. RBX1/2 expression showed their own specific characteristics in tumor pathological stages and grades, copy number variation and immune components.ConclusionsThese findings not only indicated that the difference of RBX1/2 might result in varying degrees of tumor progression, but also suggested that they might serve as biomarkers for immune infiltration in cancers, shedding new light on therapeutics of cancers.

show abstract

Section: Discussionmentioning

confidence: 99%

An integrative pan-cancer analysis revealing the difference in small ring finger family of SCF E3 ubiquitin ligases

Huang

Li²,

Liu³

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…RBX1 is overexpressed in many primary tumours, including liver cancer, non-musculoinvasive transitional cell carcinoma of the bladder (NMIBC), and lung cancer [32][33][34][35][36]. Several studies have shown that RBX1 is participated in different aspects of the cancer biology, and lots of RBX1 molecular functions are in accordance with its function in cancer [37][38][39]. Nonetheless, there exist no information on the RBX1 molecular mechanisms and its concrete role in TNBC.…”

Section: Discussionmentioning

confidence: 99%

E3 ubiquitin ligase RBX1 drives the metastasis of triple negative breast cancer through a FBXO45-TWIST1-dependent degradation mechanism

Shao

Feng

Jiang

et al. 2022

Aging

View full text Add to dashboard Cite

Triple-negative breast cancer (TNBC) patients are at high risk of recurrence and metastasis in the early stages, although receiving standard treatment. However, the underlying mechanism of TNBC remains unclear. Here, the critical effect of E3 ubiquitin ligase RBX1 in the metastasis of TNBC was reported for the first time. We discovered that RBX1 expression was evidently raised in the tissues of TNBC. Our clinical research displayed that high RBX1 expression was markedly related to poor distant invasion and survival. Functional analysis exhibited that RBX1 facilitated metastasis of TNBC cells through increasing EMT. Furthermore, we demonstrated that RBX1 knockdown increased the levels of the Twist family bHLH transcription factor 1 (TWIST1), is a significant regulator in the EMT process in some cancers. It can be observed an evident positive correlation between the TWIST1 and RBX1 levels, further confirming that EMT induced by RBX1 in TNBC cells is determined by TWIST1. Mechanistically, RBX1 modulates the expression of TWIST1 via modulating FBXO45, directly binding to FBXO45, and facilitating its degradation and ubiquitination. Briefly, our findings confirm that RBX1 is probably a new biomarker of TNBC carcinogenesis, thus suggesting that targeting the RBX1/FBXO45/TWIST1 axis may be an underlying strategy for TNBC treatment.

show abstract

“…[ 5 ] ROC1 is reported to be overexpressed in a variety of neoplasms, including liver cancer, gastric cancer, esophageal cancer, bladder cancer, non-small cell lung cancer, renal cell carcinoma (RCC), prostate cancer, ovarian cancer and melanoma. [ 5 , 12 , 15 , 25 , 26 ] Thereby we conducted this meta-analysis to explore the potential connection between ROC1 expression and its prognostic values in cancers. Our results showed that ROC1 was able to serve as a potential prognostic biomarker in multiple tumors.…”

Section: Discussionmentioning

confidence: 99%

“…[ 12 ] Several studies have indicated that overexpression of ROC1 is connected with higher Fuhrman grade and ROC1silencing remarkably hinders cancer cells growth and survival through G2/M arrest, senescence and apoptosis by accumulation of WEE1, p21, p27, NOXA, and BIM in renal cell carcinoma (RCC). [ 14 , 25 ] Elevated ROC1 expression is associated with poor clinical features in ovarian cancer and reduced expression of ROC1 can inhibit ovarian cancer cells proliferation via ubiquitination of TP53 through the miR-194-5p/ROC1 pathway, finally blocking cancer cells growth and invasion. Additionally, ROC1 is found to be associated with chromosome instability in ovarian cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological and prognostic role of ROC1 in neoplasms: A PRISMA-compliant systematic review and meta-analysis

Shen¹,

Wang²,

Qiu³

et al. 2022

Medicine

View full text Add to dashboard Cite

Background: Regulator of cullins 1 (ROC1) is frequently overexpressed in multiple tumors, and many pieces of research demonstrate that ROC1 is associated with the prognosis and development of a diversity of neoplasms and it is able to serve as a promising prognostic biomarker. Here we performed this meta-analysis to evaluate the prognostic and clinicopathological significance of ROC1 in patients suffering from cancer. Methods: We searched Pubmed, Embase, Web of Science, Chinese National Knowledge Infrastructure (CNKI), and WanFang database. The role of ROC1 in cancers was evaluated by pooled hazard ratios (HRs), odd ratios (ORs) and 95% confidence intervals (CIs). Results: In total, 9 studies including 1002 patients were enrolled in this analysis. The pooled results showed that patients with high expression of ROC1 had poor overall survival (OS) (HR: 2.04, 95% CI: 1.48–2.60, P < 0.001) and recurrence-free survival (RFS) (HR: 1.727, 95% CI: 0.965–2.488, P < 0.001). Additionally, elevated expression of ROC1 was significantly correlated with advanced clinical Tumor Node Metastasis stage (OR: 2.708, 95% CI: 1.856–3.951, P < 0.001), positive lymph node metastasis (OR: 1.968; 95% CI: 1.294–2.993, P = .002), large tumor size (OR: 1.522, 95% CI: 1.079–2.149, P = .017) and poor tumor differentiation (OR: 2.448, 95% CI: 1.793–3.344, P < 0.001). Conclusions: Elevated ROC1 expression predicted worse prognosis and advanced pathological parameters in various cancers. ROC1 was a significant prognostic biomarker for poor survival in human cancers.

show abstract

Expression of Ring Box-1 protein and its relationship with Fuhrman grade and other clinical-pathological parameters in renal cell cancer

Cited by 5 publications

References 33 publications

An integrative pan-cancer analysis revealing the difference in small ring finger family of SCF E3 ubiquitin ligases

An integrative pan-cancer analysis revealing the difference in small ring finger family of SCF E3 ubiquitin ligases

E3 ubiquitin ligase RBX1 drives the metastasis of triple negative breast cancer through a FBXO45-TWIST1-dependent degradation mechanism

Clinicopathological and prognostic role of ROC1 in neoplasms: A PRISMA-compliant systematic review and meta-analysis

Contact Info

Product

Resources

About