Expression of SERCA2a is not regulated by calcineurin or upon mechanical unloading in skeletal muscle regeneration

Zádor, Ernö; Fenyvesi, Rita; Wuytack, Frank

doi:10.1016/j.febslet.2004.12.061

Cited by 15 publications

(13 citation statements)

References 23 publications

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“…This suggests that calcineurin has no direct effect on the expression of the calcium sequestering machinery and that the fast-to-slow transformation that occurs in vivo (8) requires other factors, such as thyroid or other circulating hormones. A similar effect has been seen in regenerating soleus muscle where inhibition of calcineurin in isolated fibers prevented the accumulation of type I MHC but had no effect on the expression of the slow sarco(endo)plasmic reticulum Ca 2ϩ ATPase pump (40).…”

Section: Discussionmentioning

confidence: 70%

Cultured slow vs. fast skeletal muscle cells differ in physiology and responsiveness to stimulation

Huang¹,

Dennis²,

Baar³

2006

American Journal of Physiology-Cell Physiology

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In vitro studies have used protein markers to distinguish between myogenic cells isolated from fast and slow skeletal muscles. The protein markers provide some support for the hypothesis that satellite cells from fast and slow muscles are different, but the data are equivocal. To test this hypothesis directly, three-dimensional skeletal muscle constructs were engineered from myogenic cells isolated from fast tibialis anterior (TA) and slow soleus (SOL) muscles of rats and functionality was tested. Time to peak twitch tension (TPT) and half relaxation time (RT(1/2)) were approximately 30% slower in constructs from the SOL. The slower contraction and relaxation times for the SOL constructs resulted in left shift of the force-frequency curve compared with those from the TA. Western blot analysis showed a 60% greater quantity of fast myosin heavy chain in the TA constructs. 14 days of chronic low-frequency electrical stimulation resulted in a 15% slower TPT and a 14% slower RT(1/2), but no change in absolute force production in the TA constructs. In SOL constructs, slow electrical stimulation resulted in an 80% increase in absolute force production with no change in TPT or RT(1/2). The addition of cyclosporine A did not prevent the increase in force in SOL constructs after chronic low-frequency electrical stimulation, suggesting that calcineurin is not responsible for the increase in force. We conclude that myogenic cells associated with a slow muscle are imprinted to produce muscle that contracts and relaxes slowly and that calcineurin activity cannot explain the response to a slow pattern of electrical stimulation.

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Section: Discussionmentioning

confidence: 70%

Cultured slow vs. fast skeletal muscle cells differ in physiology and responsiveness to stimulation

Huang¹,

Dennis²,

Baar³

2006

American Journal of Physiology-Cell Physiology

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“…An explanation for this disparity may be related to the idea that distinct subsets of MHC-typed fibers are differentially sensitive to neural activation cues mediating the cellular expression of these proteins (13). On the other hand, Zador et al (66,67) showed that overexpression of the calcineurin inhibitor CAIN or partial tenotomy prevented the expression of MHC I in regenerating soleus muscle, whereas slow SERCA2 expression remained elevated. These findings reveal that the regulation of SERCA2 expression is distinct from that of the slow myosin and that it may be modulated by neuronal activity but is not entirely dependent on it.…”

Section: Discussionmentioning

confidence: 93%

Adaptive responses to creatine loading and exercise in fast-twitch rat skeletal muscle

Gallo¹,

MacLean²,

Tyreman³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

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We investigated the effects of chronic creatine loading and voluntary running (Run) on muscle fiber types, proteins that regulate intracellular Ca2+, and the metabolic profile in rat plantaris muscle to ascertain the bases for our previous observations that creatine loading results in a higher proportion of myosin heavy chain (MHC) IIb, without corresponding changes in contractile properties. Forty Sprague-Dawley rats were assigned to one of four groups: creatine-fed sedentary, creatine-fed run-trained, control-fed sedentary, and control-fed run-trained animals. Proportion and cross-sectional area increased 10% and 15% in type IIb fibers and the proportion of type IIa fibers decreased 11% in the creatine-fed run-trained compared with the control-fed run-trained group (P < 0.03). No differences were observed in fast Ca2+-ATPase isoform SERCA1 content (P > 0.49). Creatine feeding alone induced a 41% increase (P < 0.03) in slow Ca2+-ATPase (SERCA2) content, which was further elevated by 33% with running (P < 0.02). Run training alone reduced parvalbumin content by 50% (P < 0.05). By comparison, parvalbumin content was dramatically decreased by 75% (P < 0.01) by creatine feeding alone but was not further reduced by run training. These adaptive changes indicate that elevating the capacity for high-energy phosphate shuttling, through creatine loading, alleviates the need for intracellular Ca2+ buffering by parvalbumin and increases the efficiency of Ca2+ uptake by SERCAs. Citrate synthase and 3-hydroxyacyl-CoA dehydrogenase activities were elevated by run training (P < 0.003) but not by run training + creatine feeding. This indicates that creatine loading during run training supports a faster muscle phenotype that is adequately supported by the existing glycolytic potential, without changes in the capacity for terminal substrate oxidation.

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“…Muscle necrosis was induced by the in situ injection of notexin, a snake venom (Harris et al 1975;Harris 2003) with modiWcations assuring the complete degradation of each Wber (Zádor et al 1996). The ensuing regeneration process in the soleus muscle was found to be remarkably reproducible as assessed by histological inspection and by a number of biochemical parameters (Zádor et al 1996(Zádor et al , 2001(Zádor et al , 2002(Zádor et al , 2005(Zádor et al , 2007. Transfection with 30 g of SERCA1b RNAi expressing pSuperEGFP vector in 50 l of 20% sucrose on day 4 of regeneration was done, as described previously (Zádor and Wuytack 2003).…”

Section: Methodsmentioning

confidence: 99%

“…3e, f). However, we have previously shown that because of unknown reasons the MyHC1 can be abolished also in Wbers without detectable cain expression in the cain transfected regenerating soleus muscle (Zádor et al 2005). Therefore, in support of the interpretation of the distinct results received using cotransfection or subsequent transfection, we also show that cotransfection of the 1:1 mix of two plasmids (pEGFP and pDsRED) aVected the same Wbers (SI 3a), while the separate transfection of the two plasmids aVected diVerent Wbers (SI 3b).…”

Section: Serca1b-directed Rnai Stimulates Muscle Regenerationmentioning

confidence: 96%

Silencing SERCA1b in a few fibers stimulates growth in the entire regenerating soleus muscle

Zádor

Owsianik

Wuytack

2010

Histochem Cell Biol

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The neonatal isoform of the sarcoplasmic/endoplasmic reticulum Ca²(+) ATPase 1 (SERCA1b) is a dominant Ca²(+) pump in the young fibers of regenerating muscle. In vivo transfection of about 1% of the fibers with SERCA1b RNAi plasmid resulted in no apparent change in the transfected fibers, but enhanced the increase of fresh weight and fiber size in the whole regenerating rat soleus muscle, until the normal size was reached. Co-transfection of calcineurin inhibitor cain/cabin-1 with SERCA1b RNAi was sufficient to cut down the widespread growth stimulation, but the subsequent transfection of cain into the SERCA1b RNAi transfected muscle did not inhibit muscle growth. The SERCA1b RNAi preferably upregulated the expression of the NFAT reporter lacZ compared to controls when co-transfected into the fibers. Notably, perimuscular injection of interleukin-4 (IL-4) antibody but not that of an unrelevant antibody completely abolished the growth-promoting effect of SERCA1b RNAi. This indicates that silencing SERCA1b in a few fibers stimulates the calcineurin-NFAT-IL-4 pathway and fiber growth in the whole regenerating soleus. These results suggest the presence of an autocrine-paracrine coordination of growing muscle fibers, and put forward a new method to stimulate skeletal muscle regeneration.

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Expression of SERCA2a is not regulated by calcineurin or upon mechanical unloading in skeletal muscle regeneration

Cited by 15 publications

References 23 publications

Cultured slow vs. fast skeletal muscle cells differ in physiology and responsiveness to stimulation

Cultured slow vs. fast skeletal muscle cells differ in physiology and responsiveness to stimulation

Adaptive responses to creatine loading and exercise in fast-twitch rat skeletal muscle

Silencing SERCA1b in a few fibers stimulates growth in the entire regenerating soleus muscle

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