Expression of the chickpea CarNAC3 gene enhances salinity and drought tolerance in transgenic poplars

Movahedi, Ali; Zhang, Jiaxin; Gao, Pinhong; Yang, Yang; Wang, Like; Yin, Tongming; Kadkhodaei, Saeid; Ebrahimi, Mortaza; Zhuge, Qiang

doi:10.1007/s11240-014-0588-z

Cited by 49 publications

(31 citation statements)

References 39 publications

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“…The data of this study on higher proline levels in salt and PEG treated transgenics is in conformity with RoyChoudhury et al (2007), in studying the tolerance of Rab16A-overexpressing tobacco plants under the NaCl stress. Moreover, similar results have been reported by numerous researchers in other plants overexpressing Mdmyb10, CarNAC2, CarNAC3 TFs for osmotic and NaCl stress (Gao et al 2011;Movahedi et al 2014;Yu et al 2014). A decrease in free proline was noticed in SoMYB18 plants under drought at 12 and 24 h in contrast to initial rise and vice versa in salt stress; similar results were reported by overexpressing TaMYB2A in Arabidopsis (Mao et al 2011), OsMYB3R2 and OsMYB2 in rice (Ma et al 2009;El-kereamy et al 2012) under different abiotic stresses.…”

Section: Discussionsupporting

confidence: 87%

“…Similar results were reported in transgenic tobacco expressing AmMYB1 under salt and TaPIMP1 under salinity and osmotic stress Ganesan et al 2012). Mao et al (2011) reported in overexpressing Arabidopsis (Mdmyb10) under multiple abiotic stresses and Movahedi et al (2014) in poplar (CarNAC3) under drought and salt stresses. Altogether, biochemical evaluations advocate that expression of SoMYB18 in tobacco improves the stress tolerance by accretion of the defense factors (chlorophyll and proline) and by decreasing the lipid peroxidation under salt and PEG stress conditions.…”

Section: Discussionsupporting

confidence: 83%

“…9). Similarly enhanced CAT activity under abiotic stress was reported in transgenic tobacco expressing rice Rab16A (RoyChoudhury et al 2007), in rice overexpressing OsMYB2 (Yang et al 2012) and in CarNAC3overexpressing poplar plants (Movahedi et al 2014).…”

Section: Discussionsupporting

confidence: 57%

“…Our results are in consensus with the previous findings reported in several researches. Recently Movahedi et al (2014) observed a decrease in MDA levels under drought stress in CarNAC3 transgenic poplars and OsMYB48-1 overexpression in rice exhibited reduced rate of MDA content under drought and salinity stresses (Xiong et al 2014). Moreover, analogous results have been accounted in transgenic plants expressing tolerant genes in tobacco under abiotic stress (RoyChoudhury et al 2007;Wang et al 2012;Mahajan and Yadav 2014) and in Arabidopsis expressing Mdmyb10 gene in osmotic stress (Gao et al 2011).…”

Section: Discussionmentioning

confidence: 73%

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SoMYB18, a sugarcane MYB transcription factor improves salt and dehydration tolerance in tobacco

et al. 2015

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MYB transcription factors play vital role in various biotic and abiotic defense responses. A differentially expressed, stress-responsive, R2-R3-type, MYB-like transcription factor-associated EST (MYB18) from drought and salt SSH libraries was identified. A full-length DNA sequence of a MYB gene, namely SoMYB18, was isolated from sugarcane var. Co740. SoMYB18 encodes for an ORF of 1284 bp having 427 amino acids with 47.43 kDa theoretical protein size MYB protein. The phylogenetic characterization of the deduced amino acid sequence showed similarity with monocot MYB proteins. SoMYB18 is a member of the R2R3-MYB subfamily, containing two MYB DNA-binding domains (R2, R3) and a SANT/MYB DNA-binding domain. The SoMYB18 gene was transferred into tobacco, and stable transgenic plants expressing SoMYB18 were evaluated for salt, drought and cold tolerance. Compared to un-transformed tobacco plants, SoMYB18-expressing plants exhibited notably improved tolerances to salt and drought stress. The SOD and CAT activities were considerably elevated in transgenic plants, as well as proline accumulation and chlorophyll content were considerably high and lipid peroxidation was reduced relative to un-transformed plants during salt stress. These results indicated that this sugarcane R2R3-MYB transcription factor plays key role in regulating stress responses and may be implicated in the activation of salt-related genes, being useful in improving the salt stress tolerance in crops.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 73%

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SoMYB18, a sugarcane MYB transcription factor improves salt and dehydration tolerance in tobacco

et al. 2015

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“…Transgenic plants with herbicide, insect, disease, and drought resistance, and salt tolerance were obtained. However, most of the reports introduced only a single gene or a certain class of genes (Fladung et al, 2013;Klocko et al, 2014;Movahedi et al, 2015), and different agronomic traits, such as salt tolerance and insect resistance, are usually controlled by different genes. Therefore, multiple gene transformation is a feasible way to obtain a new poplar variety with multiple resistances (Yang et al, 2003;Wang et al, 2007a;Su et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Genetic transformation and expression of transgenic lines of Populus x euramericana with insect-resistance and salt-tolerance genes

Yang¹,

Wang²,

Zhang³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. We characterized new transgenic varieties of poplar with multiple insect-resistant and salt stress tolerant genes. Two insect-resistant Bacillus thuringiensis (Bt) genes, Cry1Ac and Cry3A, and a salt-tolerant gene, Betaine aldehyde dehydrogenase (BADH), were inserted into a vector, p209-Cry1Ac-Cry3A-BADH. The clone of Populus x euramericana was transformed by the vector using the Agrobacterium-mediated method. Three transgenic lines were assessed using genetic detection and resistance expression analysis. PCR revealed that exogenous genes Cry1Ac, Cry3A, BADH and selective marker gene NPTII were present in three transgenic lines. Quantitative realtime PCR (qPCR) showed significant differences in the transcriptional abundance of three exogenous genes in different lines. Results of assays for Bt toxic proteins showed that the Cry1Ac and Cry3A toxic protein content of each line was 12.83-26.32 and 2108.91-2724.79 ng/g, respectively. The Cry1Ac toxic protein content of different lines was significantly different; the Cry3A toxic protein content was about 100 times higher than that of the Cry1Ac toxic protein. The insect-resistance test revealed the mortality rate of transgenic lines to Hyphantria cunea L1 larvae varied by 42.2-66.7%, which was significantly higher than non-transgenic lines. The mortality rate of L1 and L2 Plagiodera versicolora larvae was 100%. The insecticidal effect of transgenic lines to P. versicolora larvae was higher than that to H. cunea larvae. NaCl stress tolerance of three transgenic lines under 3-6% NaCl concentration was significantly higher than that of non-transgenic lines.

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Water stress in plants

Rejeb¹,

Benzarti

Debez

et al. 2016

Water Stress and Crop Plants

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Expression of the chickpea CarNAC3 gene enhances salinity and drought tolerance in transgenic poplars

Cited by 49 publications

References 39 publications

SoMYB18, a sugarcane MYB transcription factor improves salt and dehydration tolerance in tobacco

SoMYB18, a sugarcane MYB transcription factor improves salt and dehydration tolerance in tobacco

Genetic transformation and expression of transgenic lines of Populus x euramericana with insect-resistance and salt-tolerance genes

Water stress in plants

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