Expression of the gene encoding Δ12 acyl-lipid desaturase from Synechocystis sp. PCC 6803 improves potato plant resistance to late blight infection

Yur’eva, N. O.; Kirsanova, S. N.; Кукушкина, Л. Н.; Пчелкин, В. П.; Sobolkova, G. I.; Nikiforova, Kh. R.; Goldenkova-Pavlova, I. V.; Носов, А. М.; Цыдендамбаев, В. Д.

doi:10.1134/s1021443714050161

Cited by 3 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar pattern was observed when this phytopathogen infected transgenic S. tuberosum L. (cv. Desnitsa) plants expressing the desA gene from Synechocystis [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…There was an absence of changes in the LPO level in the transgenic potato plants cv. Desnitsa expressing desA-licBM3 [ 24 ] after a hypothermic impact.…”

Section: Discussionmentioning

confidence: 99%

“…The leaves of control and transgenic potato plants were infected with the pathogen, and the degree of their resistance was assessed according to techniques which were previously described [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…The degree of infection of plant leaves was estimated using the following scale: 9 points, very high resistance (no signs of affection); 7 points, high resistance (25% of leaf surface area affected); 5 points, medium resistance (25 to 50% of leaf surface area affected); 3 points, low resistance (50–75% of leaf surface area affected); and 1 point, susceptible (over 75% of leaf surface area affected) [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…Expression of the gene of ∆12-acyl-lipid desaturase of Synechocystis sp. PCC6803 increased the in vitro resistance of the potato cultivar Desnitsa to late blight with a lower level of lipid peroxidation (LPO) as compared with the control [ 24 ]. All these facts indirectly suggest that the function of the antioxidant system is changed in the transgenic plants expressing heterologous genes of desaturases.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Response of Transgenic Potato Plants Expressing Heterologous Genes of ∆9- or ∆12-Acyl-lipid Desaturases to Phytophthora infestans Infection

Tsypurskaya

Николаева

Lapshin

et al. 2022

Plants

View full text Add to dashboard Cite

Late blight is one of the most economically important diseases affecting potato and causing a significant loss in yield. The development of transgenic potato plants with enhanced resistance to infection by Phytophthora infestans may represent a possible approach to solving this issue. A comparative study of the leaf response in control potato plants (S.tuberosum L. cultivar Skoroplodnyi), control transgenic plants expressing the reporter gene of thermostable lichenase (transgenic licBM3 line) and transgenic plants expressing cyanobacterial hybrid genes ∆9-acyl-lipid desaturase (transgenic desC lines) and ∆12-acyl-lipid desaturase (transgenic desA lines) to infection with P. infestans has been performed. The expression of desaturase genes in potato plants enhanced their tolerance to potato late blight agents as compared with the control. The lipid peroxidation level raised in the leaves of the control and transgenic desA plants on third day after inoculation with P. infestans zoospores and remained the same in the transgenic desC plants. The number of total phenolic compounds was increased as early as on the second day after infection in all studied variants and continued to remain the same, except for transgenic desC plants. Accumulation of flavonoids, the main components of the potato leaf phenolic complex, raised on the second day in all studied variants, remained unchanged on the third day in the control plants and decreased in most transgenic plants expressing desaturase genes. The results obtained in our study demonstrate that the expression of genes of Δ9- and Δ12-acyl-lipid desaturases in potato plants enhanced their resistance to P. infestans as compared with the control non-transgenic plants due to concomitant accumulation of phenolic compounds, including flavonoids, in the leaves. All these changes were more pronounced in transgenic desC plants, which indicates that the Δ9-acyllipid desaturase gene appears to be a potential inducer of the production of biological antioxidants in plant cells.

show abstract

“…A similar pattern was observed when this phytopathogen infected transgenic S. tuberosum L. (cv. Desnitsa) plants expressing the desA gene from Synechocystis [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…There was an absence of changes in the LPO level in the transgenic potato plants cv. Desnitsa expressing desA-licBM3 [ 24 ] after a hypothermic impact.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations