Expression of the Maize Dof1 Transcription Factor in Wheat and Sorghum

Pena, Pamela A.; Quach, Truyen; Sato, Shirley; Ge, Zhengxiang; Nersesian, Natalya; Changa, Taity; Dweikat, İsmail; Soundararajan, M.; Clemente, Tom

doi:10.3389/fpls.2017.00434

Cited by 56 publications

(35 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many plants display a relatively low NUE because they are ineffective at accessing soil N and may take up as little as 41% of supplied N fertilizer (Hodge et al, 2000;Kumar and Goh, 2002;Thornton and Robinson, 2005;Yang et al, 2015;Zhu et al, 2016). Research on improving NUE has focused on the manipulation of nitrate and ammonium transporters (Fraisier et al, 2000;Yuan et al, 2007;Tsay et al, 2011;Fang et al, 2013;Ranathunge et al, 2014;Bao et al, 2015;Chen et al, 2016Chen et al, , 2017Fan et al, 2016;Wang et al, 2018aWang et al, , 2018b, associated transcription factors (Qu et al, 2015;Araus et al, 2016), or the ectopic expression of genes involved in amino acid synthesis and N metabolism (Ameziane et al, 2000;Chichkova et al, 2001;Habash et al, 2001;Yamaya et al, 2002;Seiffert et al, 2004;Good et al, 2007;Shrawat et al, 2008;McAllister et al, 2012;Peña et al, 2017). Although promising results have been achieved with respect to seed yields and NUE, these studies rarely analyzed both NUE-contributing factors, NUpE and NUtE, or the effects of different N environments (Kiba and Krapp, 2016;Tegeder and Masclaux-Daubresse, 2018).…”

Section: Leaf Amino Acid Supply Affects Photosynthetic and Plant Nitrmentioning

confidence: 99%

Leaf Amino Acid Supply Affects Photosynthetic and Plant Nitrogen Use Efficiency under Nitrogen Stress

2018

View full text Add to dashboard Cite

The coordinated distribution of nitrogen to source leaves and sinks is essential for supporting leaf metabolism while also supplying sufficient nitrogen to seeds for development. This study aimed to understand how regulated amino acid allocation to leaves affects photosynthesis and overall plant nitrogen use efficiency in Arabidopsis () and how soil nitrogen availability influences these processes. Arabidopsis plants with a knockout of , encoding an amino acid permease involved in xylem-to-phloem transfer of root-derived amino acids, were grown in low-, moderate-, and high-nitrogen environments. We analyzed nitrogen allocation to shoot tissues, photosynthesis, and photosynthetic and plant nitrogen use efficiency in these knockout plants. Our results demonstrate that, independent of nitrogen conditions, plants allocate more nitrogen to leaves than wild-type plants. Increased leaf nitrogen supply positively affected chlorophyll and Rubisco levels, photosynthetic nitrogen use efficiency, and carbon assimilation and transport to sinks. The plants outperformed wild-type plants with respect to growth, seed yield and carbon storage pools, and nitrogen use efficiency in both high and deficient nitrogen environments. Overall, this study demonstrates that increasing nitrogen allocation to leaves represents an effective strategy for improving carbon fixation and photosynthetic nitrogen use efficiency. The results indicate that an optimized coordination of nitrogen and carbon partitioning processes is critical for high oilseed production in Arabidopsis, including in plants exposed to limiting nitrogen conditions.

show abstract

Section: Leaf Amino Acid Supply Affects Photosynthetic and Plant Nitrmentioning

confidence: 99%

Leaf Amino Acid Supply Affects Photosynthetic and Plant Nitrogen Use Efficiency under Nitrogen Stress

2018

View full text Add to dashboard Cite

show abstract

“…However, efforts to increase N uptake and NUpE through the manipulation of nitrate and ammonium transporters or associated transcription factors have had only limited success (Fraisier et al, 2000;Yuan et al, 2007;Tsay et al, 2011;Ranathunge et al, 2014;Qu et al, 2015;Araus et al, 2016;Chen et al, 2017). Furthermore, varying outcomes have been reported from the ectopic expression of genes involved in amino acid synthesis and N metabolism (Ameziane et al, 2000;Chichkova et al, 2001;Habash et al, 2001;Yamaya et al, 2002;Seiffert et al, 2004;Good et al, 2007;Shrawat et al, 2008;McAllister et al, 2012;Peña et al, 2017). However, these studies rarely addressed the different components of NUE (i.e.…”

Section: Nue Is Greatly Improved In Aap1-oe Plantsmentioning

confidence: 99%

“…Nitrogen utilization efficiency (NUtE) is defined as total seed yield relative to total shoot N content (Moll et al, 1982) and is affected by several physiological factors, including N uptake, metabolism, allocation, and remobilization (Habash et al, 2001;Tsay et al, 2011;Girondé et al, 2015). In addition, regulated transport of N within the plant strongly influences the amount of N allocated to seeds and final seed yields (Rolletschek et al, 2005;Schmidt et al, 2007;Weigelt et al, 2008;Tan et al, 2010;Zhang et al, 2010Zhang et al, , 2015Carter and Tegeder, 2016;Santiago and Tegeder, 2016 Araus et al, 2016;Chen et al, 2017), nitrate allocation (Tsay et al, 2011), N metabolism (Ameziane et al, 2000Chichkova et al, 2001;Habash et al, 2001;Yamaya et al, 2002;Seiffert et al, 2004;Good et al, 2007;Shrawat et al, 2008;Peña et al, 2017), and its regulation (Yanagisawa et al, 2004). While generally successful, these studies often did not address the different components of NUE (i.e.…”

mentioning

confidence: 99%

“…Approaches to improve plant NUE have focused mainly on the genetic manipulation of N uptake (Fang et al, 2013; Araus et al, 2016;Chen et al, 2017), nitrate allocation (Tsay et al, 2011), N metabolism (Ameziane et al, 2000;Chichkova et al, 2001;Habash et al, 2001;Yamaya et al, 2002;Seiffert et al, 2004;Good et al, 2007;Shrawat et al, 2008;Peña et al, 2017), and its regulation (Yanagisawa et al, 2004). While generally successful, these studies often did not address the different components of NUE (i.e.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Improving Plant Nitrogen Use Efficiency through Alteration of Amino Acid Transport Processes

2017

View full text Add to dashboard Cite

Improving the efficiency of nitrogen (N) uptake and utilization in plants could potentially increase crop yields while reducing N fertilization and, subsequently, environmental pollution. Within most plants, N is transported primarily as amino acids. In this study, pea (Pisum sativum) plants overexpressing AMINO ACID PERMEASE1 (AAP1) were used to determine if and how genetic manipulation of amino acid transport from source to sink affects plant N use efficiency. The modified plants were grown under low, moderate, or high N fertilization regimes. The results showed that, independent of the N nutrition, the engineered plants allocate more N via the vasculature to the shoot and seeds and produce more biomass and higher seed yields than wild-type plants. Dependent on the amount of N supplied, the AAP1-overexpressing plants displayed improved N uptake or utilization efficiency, or a combination of the two. They also showed significantly increased N use efficiency in N-deficient as well as in N-rich soils and, impressively, required half the amount of N to produce as many fruits and seeds as control plants. Together, these data support that engineering N allocation from source to sink presents an effective strategy to produce crop plants with improved productivity as well as N use efficiency in a range of N environments.Nitrogen (N) is an essential nutrient that plants require in large amounts for growth and development. In industrial countries, high N fertilization enables maximum crop yields, and in the last 50 years, the use of synthetic N fertilizers has increased dramatically to meet the agricultural demands of a growing population (FAO, 2012;Conant et al., 2013). However, depending on the crop species and soil conditions, plants take up less than half of the applied N fertilizer (Raun and Johnson, 1999;Hodge et al., 2000;Kumar and Goh, 2002;Yang et al., 2015;Zhu et al., 2016). The remaining soil N may contaminate aquatic systems through runoffs or leached water (Crews and Peoples, 2005;Gruber and Galloway, 2008) or it may undergo denitrification and be released into the atmosphere as nitrous oxide, a powerful greenhouse gas (Bouwman, 1996;Bouwman et al., 2002), altogether resulting in negative effects on the environment and human health. Oppositely, in developing countries, access to N fertilizer is limited, and insufficient N nutrition results in low crop productivity and, ultimately, in reduced food supply (Brown et al., 2009;Lal, 2009).One solution to these issues is the production of crop varieties that are highly efficient in using N and produce high yields with reduced N input (MasclauxDaubresse et al., 2010;McAllister et al., 2012;Garnett et al., 2015). Plant nitrogen use efficiency (NUE) is determined by plant seed yield relative to the amount of N applied and is generally composed of both N uptake and N utilization efficiency (Moll et al., 1982). Nitrogen uptake efficiency (NUpE) is defined as total shoot N relative to the amount of N supplied to the soil. The uptake of N by the root is influenced by th...

show abstract

“…Many genes involved in stress response can be simultaneously regulated by a single gene encoding stress inducible transcription factor (Kasuga et al, 1999). While the expression of ZmDof1 transcription factor in sorghum and wheat has activated the primary target, phosphoenolpyruvate carboxylase (PEPC), which leads to the down-regulation of genes involved in photosynthesis and the functional apparatus of chloroplasts, and negatively impacted photosynthesis, and biomass (Peña et al, 2017). These results indicated that transcription factor strategies for crop improvement need to consider the downstream targets of the genetic elements to be introduced.…”

Section: Discussionmentioning

confidence: 99%

The impact of over-expression of NPK1 gene on growth and yield of sorghum under drought stress

Assem¹,

Zamzam²,

Saad³

et al. 2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Sorghum bicolor L. is an important crop in many tropical regions, yet it has received little attention in applying modern biotechnologies for improvement due to transformation difficulties. Drought is among the most important factors limiting sorghum productivity. The Nicotina Protein Kinase 1 (NPK1) gene confers tolerance to adverse environmental conditions. The present work was conducted to determine the amenability of sorghum genotypes to Agrobacterium-mediated transformation with the NPK1 gene and to characterize the role of that gene in S. bicolor. The NPK1 gene along with the bar gene was successfully introduced into two sorghum genotypes: Dorado and SPGM94021. The transformation frequencies were 1.49 and 1.79% for Dorado and SPGM94021, respectively. Stable integration of the transgenes in T0 and T1 plants was confirmed through polymerase chain reaction (PCR) and Southern blotting. The NPK1 gene expression was measured through real-time-PCR. T1 plants tested in vitro for tolerance to mannitol osmotic stress maintaining a higher growth rate and showed increased tolerance to stress conditions compared to the nontransgenic plants. The transgenic sorghum had a significantly higher kernel weight under drought stress conditions than the control plants. Accordingly, the NPK1 gene might induce a mechanism that protects sorghum plants against possible water-deficiency stress conditions.

show abstract

Expression of the Maize Dof1 Transcription Factor in Wheat and Sorghum

Cited by 56 publications

References 63 publications

Leaf Amino Acid Supply Affects Photosynthetic and Plant Nitrogen Use Efficiency under Nitrogen Stress

Leaf Amino Acid Supply Affects Photosynthetic and Plant Nitrogen Use Efficiency under Nitrogen Stress

Improving Plant Nitrogen Use Efficiency through Alteration of Amino Acid Transport Processes

The impact of over-expression of NPK1 gene on growth and yield of sorghum under drought stress

Contact Info

Product

Resources

About