Expression of Yeast Hem1 Controlled by Arabidopsis Hema1 Promoter Enhances Leaf Photosynthesis in Transgenic Tomato

Zhang, Zhi Ping; Sheng, Ming; Li, Chui; Sun, Xin; Yao, Quan Hong; Wang, Liang Ju

doi:10.17660/actahortic.2012.932.44

Cited by 4 publications

(2 citation statements)

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“…In addition to research on exogenous ALA application, studies on transgenic plants that overproduce endogenous ALA have confirmed its biological functions. When Yhem1 (a yeast Hem1 driven by the Arabidopsis HemA promoter, which is light-inducible) was transformed into higher plants, transgenic tobacco [ 76 , 77 ], strawberry [ 78 ], tomato [ 79 ], and canola [ 80 ] biosynthesized much more endogenous ALA and chlorophyll and had higher photosynthetic rates and energy conversion efficiency than their respective WTs. Yhem1 transgenic Arabidopsis [ 81 ], tomato [ 82 ], and canola [ 83 ] have been demonstrated to be more salt tolerant, and transgenic banana was colder tolerant [ 84 ].…”

Section: Discussionmentioning

confidence: 99%

ALA reverses ABA-induced stomatal closure by modulating PP2AC and SnRK2.6 activity in apple leaves

Chen

Wang

2023

Horticulture Research

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5-Aminolevulinic acid (ALA), known as a new natural plant growth regulator, can reverse abscisic acid (ABA)-induced stomatal closure. The protein phosphatase 2A (PP2A) played an important role in regulation of stomatal movement by ALA and ABA; however, the underlying molecular mechanisms remain unclear. Here, we report that ALA promotes MdPP2A activity and gene expression in the leaf epidermis of apple (Malus × domestica Borkh.), and expression of the catalytic subunit MdPP2AC was most significantly correlated with stomatal aperture. Western blotting showed that ALA enhanced MdPP2AC protein abundance and phosphorylation. Y2H (yeast two hybrid), FLC (firefly luciferase complementation imaging) and BiFC (Bimolecular fluorescence complementation) assays showed that MdPP2AC interacted with several other MdPP2A subunits as well as MdSnRK2.6 (Sucrose non-fermenting 1-related protein kinase 2.6), and the latter interaction was further verified by pull-down and MST (microscale thermophoresis) assays. ALA downregulated ABA-induced MdSnRK2.6 gene expression, kinase activity, and protein phosphorylation. In transiently transgenic apple leaves, OE-MdPP2AC promoted stomatal aperture by reducing Ca2+ and H2O2 levels but increasing flavonol levels in guard cells. Conversely, OE-MdSnRK2.6 induced stomatal closure by increasing Ca2+ and H2O2 but reducing flavonols. Partial silencing of these genes had opposite effects on Ca2+, H2O2, flavonols, and stomatal movement. Application of exogenous ALA stimulated PP2A activity, which promoted SnRK2.6 dephosphorylation and lower kinase activity in wild-type and transgenic apple leaves. We therefore propose that PP2AC, which dephosphorylates SnRK2.6 and represses its enzyme activity, mediates ALA signaling to inhibit ABA-induced stomatal closure in apple leaves.

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Section: Discussionmentioning

confidence: 99%

ALA reverses ABA-induced stomatal closure by modulating PP2AC and SnRK2.6 activity in apple leaves

Chen

Wang

2023

Horticulture Research

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“…Besides, it often also accompany by high or low voltage situations. [4] The frequency will decline when the active power of isolated power system is insufficient. If the unit s' spinning reserve is adequate and adjustion is fast, the frequency can be restored to nearly normal values in a few seconds.…”

Section: The Factors Of Influencing the Isolated Power Systemmentioning

confidence: 99%

The Study on Isolated Power Control System Based on Coordinated Control

2012

AMM

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This paper analysis the formation conditions of isolated power system, its influencing factors, revealing the operation mode, automatic safety device, generator protection regulation characteristics and these factors to stable operation of isolated power system. The dynamic models for isolated power system with generating unit of medium and small capacity are proposed in the paper to meet the need of the applicable ones to study their dynamic characteristics. The differences between isolated and the interconnected power system in the frequency characteristics are proposed and consider into low-frequency load shedding scheme and optimization methods that can meet both isolated and the interconnected power system frequency stability requirements. Not only more proper control strategy of units but their evaluation indexes are suggested to improve performance and achieve stability in islanded power system.

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Genetic diversity of melon landraces (Cucumis melo L.) in the Xinjiang Uygur Autonomous Region on the basis of simple sequence repeat markers

Zhang

Fan

Aierken

et al. 2016

Genet Resour Crop Evol

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Expression of Yeast Hem1 Controlled by Arabidopsis Hema1 Promoter Enhances Leaf Photosynthesis in Transgenic Tomato

Cited by 4 publications

References 0 publications

ALA reverses ABA-induced stomatal closure by modulating PP2AC and SnRK2.6 activity in apple leaves

ALA reverses ABA-induced stomatal closure by modulating PP2AC and SnRK2.6 activity in apple leaves

The Study on Isolated Power Control System Based on Coordinated Control

Genetic diversity of melon landraces (Cucumis melo L.) in the Xinjiang Uygur Autonomous Region on the basis of simple sequence repeat markers

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