Expression pattern of a murine homeobox gene, Dbx, displays extreme spatial restriction in embryonic forebrain and spinal cord.

Lu, Sangwei; Bogarad, Leonard D.; Murtha, Michael T.; Ruddle, Frank H.

doi:10.1073/pnas.89.17.8053

Cited by 74 publications

(46 citation statements)

References 22 publications

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“…The Eux-1 and -2,0tx-1 and -2, and NK family of homeobox genes are also expressed in the developing forebrain (Dush and Martin, 1992;Saha et al, 1993;Simeone et al, 1993). Furthermore, the Dbx homeobox gene is expressed in the telencephalon, diencephalon, hindbrain, and spinal cord (Lu et al, 1992).…”

Section: Gsh-imentioning

confidence: 99%

“…The clustered Hox genes are clearly implicated in the development of the hindbrain, but these genes are not expressed in the more rostra1 regions of the developing brain. Increasing evidence suggests that the dispersed homeobox genes play critical roles in the formation of the forebrain and the midbrain, with several now reported to have restricted domains of expression in the developing brain (Porteus et al, 1991;Lu et al, 1992;Simeone et al, 1993;Saha et al, 1993;Dush and Martin, 1992), and with a targeted mutation in the Engrailed-1 gene resulting in the deletion of certain midbrain structures (Wurst et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Gsh‐1: A novel murine homeobox gene expressed in the central nervous system

Valerius

Stock

et al. 1995

Developmental Dynamics

103

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We report the characterization of Gsh-1, a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in sue present at embryonic days 10.5, 11.5, and 12.5 of development. The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh-1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh-1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh-I homeodomain, we have determined the consensus DNA binding site of the Gsh-1 homeoprotein to be GCT/cA/,ATTAG/A. 0 1995 Wiley-Liss, Inc.

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Section: Gsh-imentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gsh‐1: A novel murine homeobox gene expressed in the central nervous system

Valerius

Stock

et al. 1995

Developmental Dynamics

103

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“…Images were obtained using a cooled CCD camera (DC500; Leica, Nussloch, Germany). The following cDNA probes were used in this study: Dbx1 (Lu et al, 1992), Dlx2 (Liu et al, 1997), Couptf1 (Qiu et al, 1994), Er81 (Lin et al, 1998), Gsh2 (Szucsik et al, 1997), Lhx2 (Rincon-Limas et al, 1999), Lhx5 (Zhao et al, 1999), Lhx6 and Lhx7 (Grigoriou et al, 1998), Ngn2 (Gradwohl et al, 1996), Nkx2-1 (Shimamura et al, 1995), Nkx2-2 ), Nkx6-2 (Qiu et al, 1998, Pax6 (Walther and Gruss, 1991), and Shh (Echelard et al, 1993).…”

Section: Methodsmentioning

confidence: 99%

Delineation of Multiple Subpallial Progenitor Domains by the Combinatorial Expression of Transcriptional Codes

Flames¹,

Pla²,

Gelman³

et al. 2007

J. Neurosci.

525

792

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The mammalian telencephalon is considered the most complex of all biological structures. It comprises a large number of functionally and morphologically distinct types of neurons that coordinately control most aspects of cognition and behavior. The subpallium, for example, not only gives rise to multiple neuronal types that form the basal ganglia and parts of the amygdala and septum but also is the origin of an astonishing diversity of cortical interneurons. Despite our detailed knowledge on the molecular, morphological, and physiological properties of most of these neuronal populations, the mechanisms underlying their generation are still poorly understood. Here, we comprehensively analyzed the expression patterns of several transcription factors in the ventricular zone of the developing subpallium in the mouse to generate a detailed molecular map of the different progenitor domains present in this region. Our study demonstrates that the ventricular zone of the mouse subpallium contains at least 18 domains that are uniquely defined by the combinatorial expression of several transcription factors. Furthermore, the results of microtransplantation experiments in vivo corroborate that anatomically defined regions of the mouse subpallium, such as the medial ganglionic eminence, can be subdivided into functionally distinct domains.

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“…Studies in a variety of vertebrate species have described the extrinsic and intrinsic cues necessary for establishing the progenitor domains. For example, Dbx genes encode a family of homeodomain transcription factors that define an intermediate spinal progenitor domain (Lu et al, 1992). This gene family has multiple functions in spinal cord development.…”

Section: Introductionmentioning

confidence: 99%

Regulation and function of Dbx genes in the zebrafish spinal cord

Gribble

Nikolaus

Dorsky

2007

Developmental Dynamics

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Dbx homeodomain proteins are important for spinal cord dorsal/ventral patterning and the production of multiple spinal cord cell types. We have examined the regulation and function of Dbx genes in the zebrafish. We report that Hedgehog signaling is not required for the induction or maintenance of these genes; in the absence of Hedgehog signaling, dbx1a/1b/2 are expanded ventrally with concomitant increases in postmitotic neurons that differentiate from this domain. Also, we find that retinoic acid signaling is not required for the induction of Dbx expression. Furthermore, we are the first to report that knockdown of Dbx1 function causes a dorsal expansion of nkx6.2, which is thought to be the cross-repressive partner of Dbx1 in mouse. Our data confirm that the dbx1a/1b/2 domain in zebrafish spinal cord development behaves similarly to amniotes, while extending knowledge of Dbx1 function in spinal cord patterning. Developmental Dynamics 236:3472-3483, 2007.

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Expression pattern of a murine homeobox gene, Dbx, displays extreme spatial restriction in embryonic forebrain and spinal cord.

Cited by 74 publications

References 22 publications

Gsh‐1: A novel murine homeobox gene expressed in the central nervous system

Gsh‐1: A novel murine homeobox gene expressed in the central nervous system

Delineation of Multiple Subpallial Progenitor Domains by the Combinatorial Expression of Transcriptional Codes

Regulation and function of Dbx genes in the zebrafish spinal cord

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