Expression pattern of bcar3, a downstream target of Gata2, and its binding partner, bcar1, during Xenopus development

Green, Yangsook Song; Kwon, Sunjong; Christian, Jan L.

doi:10.1016/j.gep.2015.11.004

Cited by 5 publications

(4 citation statements)

References 51 publications

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“…The use of Xenopus eggs and embryos for in vivo analyses of disease gene expression and function has exploded in recent years. This model has supported important advances in our understanding of: neurological disorders including autism, Alzheimer's disease, and depression (James et al, ; Park et al, ; Ramirez‐Vizcarrando, Hasan, Gu, Khakhalin, & Aizenman, ; Ullah, Demuro, Parker, & Pearson, ); cancers (Green, Kwon, & Christian, ; Haynes‐Gilmore et al, ; Van Nieuwenhuysen et al, ; Wei et al, ); congenital heart defects (Endicott, Basu, Khokha, & Brueckner, ; Silva et al, ; Torres‐Prioris, Smith, Mohun, Fernández, & Durán, ); craniofacial and auditory malformations (Dickinson, ; Griffin, Sondalle, Del Viso, Baserga, & Khokha, ; Moody, Neilson, Kenyon, Alfandari, & Pignoni, ; Ramírez‐Gordillo et al, ); diabetes (Kofent & Spagnoli, ; Pearl, Jarikji, & Horb, ; Salanga & Horb, ); kidney disease (Desgrange & Cereghini, ; Lienkamp, ; Stiburkova, Stekrova, Nakamura, & Ichida, ); Lennox–Gastaut syndrome (Hammer, Ebert, Jensen, & Jensen, ); and Zimmermann–Laband syndrome (Kortüm et al, ).…”

Section: Xenopus Is a Powerful System For Deciphering The Function Ofmentioning

confidence: 83%

Using Xenopus to understand human disease and developmental disorders

Sater

Moody

2017

Genesis

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Model animals are crucial to biomedical research. Among the commonly used model animals, the amphibian, Xenopus, has had tremendous impact because of its unique experimental advantages, cost effectiveness, and close evolutionary relationship with mammals as a tetrapod. Over the past 50 years, the use of Xenopus has made possible many fundamental contributions to biomedicine, and it is a cornerstone of research in cell biology, developmental biology, evolutionary biology, immunology, molecular biology, neurobiology, and physiology. The prospects for Xenopus as an experimental system are excellent: Xenopus is uniquely well-suited for many contemporary approaches used to study fundamental biological and disease mechanisms. Moreover, recent advances in high throughput DNA sequencing, genome editing, proteomics, and pharmacological screening are easily applicable in Xenopus, enabling rapid functional genomics and human disease modeling at a systems level.

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Section: Xenopus Is a Powerful System For Deciphering The Function Ofmentioning

confidence: 83%

Using Xenopus to understand human disease and developmental disorders

Sater

Moody

2017

Genesis

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“…Integrins bind its cytoplasmic tail to ZYX through alpha-actinin [27]. BCAR3 along with its binding partner BCAR1 leads to changes in cellular morphology, motility, and adhesion by the transduction of integrin signaling [28]. To check the global phosphorylation status in aggressive non-type we carried out a pathway analysis with the set of all the phosphopeptides quantified across all cell lines (2806 phosphopeptides).…”

Section: Resultsmentioning

confidence: 99%

Phosphoproteomic Profiling Identifies Aberrant Activation of Integrin Signaling in Aggressive Non-Type Bladder Carcinoma

Deb

Puttamallesh

Gondkar

et al. 2019

JCM

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Bladder carcinoma is highly heterogeneous and its complex molecular landscape; thus, poses a significant challenge for resolving an effective treatment in metastatic tumors. We computed the epithelial-mesenchymal transition (EMT) scores of three bladder carcinoma subtypes—luminal, basal, and non-type. The EMT score of the non-type indicated a “mesenchymal-like” phenotype, which correlates with a relatively more aggressive form of carcinoma, typified by an increased migration and invasion. To identify the altered signaling pathways potentially regulating this EMT phenotype in bladder cancer cell lines, we utilized liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based phosphoproteomic approach. Bioinformatics analyses were carried out to determine the activated pathways, networks, and functions in bladder carcinoma cell lines. A total of 3125 proteins were identified, with 289 signature proteins noted to be differentially phosphorylated (p ≤ 0.05) in the non-type cell lines. The integrin pathway was significantly enriched and five major proteins (TLN1, CTTN, CRKL, ZYX and BCAR3) regulating cell motility and invasion were hyperphosphorylated. Our study reveals GSK3A/B and CDK1 as promising druggable targets for the non-type molecular subtype, which could improve the treatment outcomes for aggressive bladder carcinoma.

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“…1E). By contrast, gata2 is maternally expressed and zygotic transcripts are enriched in ectoderm and ventral mesoderm during gastrulation (Green et al, 2016;Walmsley et al, 1994).…”

Section: Results Tril Is a Downstream Target Of Gata2 That Is Expressmentioning

confidence: 99%

Tril targets Smad7 for degradation to allow hematopoietic specification in Xenopus embryos

et al. 2016

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In Xenopus laevis, Bone morphogenetic proteins induce expression of the transcription factor Gata2 during gastrulation, and Gata2 is required in both ectodermal and mesodermal cells to enable mesoderm to commit to a hematopoietic fate. In the current studies, we identify tril as a Gata2 target gene that is required in both ectoderm and mesoderm for primitive hematopoiesis to occur. Tril is a transmembrane protein that functions as a co-receptor for Toll like receptors to mediate innate immune responses in the adult brain, but developmental roles for this molecule have not been identified. We show that Tril function is required both upstream and downstream of Bmp receptor mediated Smad1 phosphorylation for induction of Bmp target genes. Mechanistically, Tril triggers degradation of the Bmp inhibitor, Smad7. Tril-dependent down regulation of Smad7 relieves repression of endogenous Bmp signaling during gastrulation and this enables mesodermal progenitors to commit to a blood fate. Thus, Tril is a novel component of a Bmp-Gata2 positive feedback loop that plays an essential role in hematopoietic specification.

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Expression pattern of bcar3, a downstream target of Gata2, and its binding partner, bcar1, during Xenopus development

Cited by 5 publications

References 51 publications

Using Xenopus to understand human disease and developmental disorders

Using Xenopus to understand human disease and developmental disorders

Phosphoproteomic Profiling Identifies Aberrant Activation of Integrin Signaling in Aggressive Non-Type Bladder Carcinoma

Tril targets Smad7 for degradation to allow hematopoietic specification in Xenopus embryos

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