Expression pattern of microRNAs in ovine endometrium during the peri-implantation

Köse, Mehmet; Hitit, Mustafa; Kaya, Muhsin; Kırbaş, Mesut; Dursun, Şükrü; Alak, Ilyas; Atlı, Mehmet Osman

doi:10.1016/j.theriogenology.2022.07.015

Cited by 8 publications

(9 citation statements)

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“…We also showed that oar-miR-30d and oar-miR-379-5p were higher in P16 than in C16. Because the capability to detect miRNAs for endometrium has enabled us to reveal pregnancy-related markers ( Kose et al, 2022 ), it is intriguing to monitor common miRNAs for conceptuses and maternal bloodstream. As such, broadly conserved miRNAs (Let-7b) have been detected at different stages of pregnancy in trophoblast ( Liu et al, 2015 ) and serum ( Ioannidis and Donadeu, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

“…Moreover, gene expression profiles concerning embryo implantation in ovine endometrium revealed pregnancy-associated miRNAs, of which many were differentially expressed in the endometrium on day 13 of pregnancy ( Kiyma et al, 2015 ). Also, we determined differentially expressed miRNA profiles in the endometrium during the early pregnancy on days 12, 16, and 22 ( Kose et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

et al. 2022

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MicroRNA (miRNA) plays an important role in the control of gene expression and is implied in many biological functions, including embryo implantation and development. The aim was to assess plasma miRNA profiles during the peri-implantation and ascertain potential candidate miRNA markers for early pregnancy diagnosis in ovine plasma. The plasma samples were obtained from a total of 24 ewes on days 12 (pre-implantation; P12, n = 4), 16 (implantation; P16, n = 4) and 22 (post-implantation; P22, n = 4) after mating, and on their corresponding days of 12 (Pre-C; C12, n = 4), 16 (Imp-C; C16, n = 4) and 22 (Post-C; C22, n = 4) of the estrous cycle. The miRNA profiles in plasma were assessed by microarray technology. We detected the presence of 60 ovine-specific miRNAs in plasma samples. Of these miRNAs, 22 demonstrated a differential expression pattern, especially between the estrous cycle and early pregnancy, and targeted 521 genes. Two miRNAs (oar-miR-218a and oar-miR-1185-3p) were confirmed using RT-qPCR in the ovine plasma samples. Protein-protein interaction (PPI) network of target genes established six functional modules, of which modules 1 and 3 were enriched in the common GO terms, such as inflammatory response, defense response, and regulation of immune response. In contrast, module 2 was enriched in the developmental process involved in reproduction, embryo development, embryonic morphogenesis, and regulation of the developmental process. The results indicate that miRNAs profiles of plasma seemed to be modulated during the peri-implantation stage of pregnancy in ewes. Circulating miRNAs could be promising candidates for diagnosis in early ovine pregnancy.

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Section: Discussionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

et al. 2022

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“…Among the traits, phenotypes associated with reproductive outcome and fertility are of major interest to cattle producers, mainly because of the high production loss from a heifer of low reproductive potential. Specifically in reproductive biology, miRNAs have been demonstrated to be important regulators of embryonic development in humans and livestock, such as predicting pregnancy outcomes ( Ioannidis and Donadeu, 2016 ; Hitit et al, 2022 ), embryo viability ( Pohler et al, 2017 ) and implantation ( Kose et al, 2022 ), and endometrial receptivity ( Zang et al, 2021 ). Moreover, miRNAs regulate the gene targets by repressing or stimulating their expression ( Orang et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

miRNA expression profiles of peripheral white blood cells from beef heifers with varying reproductive potential

et al. 2023

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Reproductive performance is the most critical factor affecting production efficiency in the cow-calf industry. Heifers with low reproductive efficiency may fail to become pregnant during the breeding season or maintain a pregnancy. The cause of reproductive failure often remains unknown, and the non-pregnant heifers are not identified until several weeks after the breeding season. Therefore, improving heifer fertility utilizing genomic information has become increasingly important. One approach is using microRNAs (miRNA) in the maternal blood that play an important role in regulating the target genes underlying pregnancy success and thereby in selecting reproductively efficient heifers. Therefore, the current study hypothesized that miRNA expression profiles from peripheral white blood cells (PWBC) at weaning could predict the future reproductive outcome of beef heifers. To this end, we measured the miRNA profiles using small RNA-sequencing in Angus-Simmental crossbred heifers sampled at weaning and retrospectively classified as fertile (FH, n = 7) or subfertile (SFH, n = 7). In addition to differentially expressed miRNAs (DEMIs), their target genes were predicted from TargetScan. The PWBC gene expression from the same heifers were retrieved and co-expression networks were constructed between DEMIs and their target genes. We identified 16 differentially expressed miRNAs between the groups (p-value ≤0.05 and absolute (log2 fold change ≥0.05)). Interestingly, based on a strong negative correlation identified from miRNA-gene network analysis with PCIT (partial correlation and information theory), we identified miRNA-target genes in the SFH group. Additionally, TargetScan predictions and differential expression analysis identified bta-miR-1839 with ESR1, bta-miR-92b with KLF4 and KAT2B, bta-miR-2419-5p with LILRA4, bta-miR-1260b with UBE2E1, SKAP2 and CLEC4D, and bta-let-7a-5p with GATM, MXD1 as miRNA-gene targets. The miRNA-target gene pairs in the FH group are over-represented for MAPK, ErbB, HIF-1, FoxO, p53, mTOR, T-cell receptor, insulin and GnRH signaling pathways, while those in the SFH group include cell cycle, p53 signaling pathway and apoptosis. Some miRNAs, miRNA-target genes and regulated pathways identified in this study have a potential role in fertility; other targets are identified as novel and need to be validated in a bigger cohort that could help to predict the future reproductive outcomes of beef heifers.

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“…However, the molecular mechanisms underlying the effects of different uterine morphology on sheep reproductive performance remain unclear. At present, there are many reports focused on non-coding RNA in the regulation of endometrial functions [27,28], and the expression pattern of miRNAs in ovine endometrium was identi ed demonstrated that miRNAs modulate ovine endometrium during the peri-implantation [29,30]. To better understand the molecular mechanisms underlying the high proli cacy of Hu sheep, miRNA sequencing was performed.…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity

Dong

Jiang

Liu

et al. 2023

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Background MicroRNAs (miRNAs) play an important regulatory role in mammalian reproduction. Currently, most studies are primarily concentrated on ovarian miRNAs, ignoring the influence of uterine miRNAs on the fecundity of female sheep. To uncover potential regulators of sheep fecundity, RNA-seq was used to comparatively analyze miRNA expression profiles of endometrium between high prolificacy sheep (HP, litter size = 3) and low prolificacy sheep (LP, litter size = 1) with FecBB genotype. Results Firstly, genomic features of miRNAs from endometrium were analyzed. Furthermore, 58 differentially expressed (DE) miRNAs were found in the endometrium of Hu sheep with different litter size. A co-expression network of DE miRNAs and target genes has been constructed, and hub genes related litter size are included, such as DE miRNA unconservative_NC_019472.2_1229533 and unconservative_NC_019481.2_1637827 target to ESR1 and unconservative_NC_019481.2_1637827 targets to TCF7. Moreover, functional annotation analysis showed that the target genes (NRCAM and NEGR1) of the DE miRNAs were significantly enriched in cell adhesion molecules (CAMs) signaling pathway, which was related to uterine receptivity. Conclusion Taken together, this study provides a new valuable resource for understanding the molecular mechanisms underlying Hu sheep prolificacy.

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Expression pattern of microRNAs in ovine endometrium during the peri-implantation

Cited by 8 publications

References 73 publications

Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

miRNA expression profiles of peripheral white blood cells from beef heifers with varying reproductive potential

Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity

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