Expression pattern of zygote arrest 1 (ZAR1), maternal antigen that embryo requires (MATER), growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) genes in ovine oocytes and in vitro-produced preimplantation embryos

Bebbere, Daniela; Bogliolo, Luisa; Ariu, Federica; Fois, Stefano; Leoni, Giovanni; Tore, Silvia; Succu, Sara; Berlinguer, Fiammetta; Naitana, Salvatore; Ledda, Sergio

doi:10.1071/rd08095

Cited by 39 publications

(31 citation statements)

References 43 publications

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“…We have analysed the expression pattern of four MEGs during sheep pre-implantation development [29]. Results showed that the relative abundance of ZAR1, MATER, BMP15 and GDF9 is dynamically regulated during the window from meiotic maturation through EGA, in accordance with data in cattle [30].…”

Section: Maternal Effect Genes: Expression Patterns In Animal Modelssupporting

confidence: 62%

Unveiling mRNA Changes During Meiotic Progression and Pre-Implantation Development: Help from Large Animal Models

Ledda

Bebbere²,

Ariu³

et al. 2012

CPD

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Assisted reproductive technologies (ART) are successfully applied in several mammals, including humans, thanks to the ability of oocytes and embryos to face maturation, fertilization and first development in vitro. However, efficiency and safety of ART represent main issues. Mammalian oocytes and early embryos are transcriptionally inactive, and rely exclusively on maternal RNAs and proteins, deposited during oocyte growth, until embryonic genome activation (EGA). Such transcriptional quiescence needs complex post-transcriptional and post-translational mechanisms to coordinate meiotic maturation, fertilization, and reprogramming of the nascent genome. These events are the final outcome of complex, hormonally regulated biological processes that translate into specific molecular mechanisms, which are still far from being fully understood. A deep knowledge of these early phases of development is crucial to understand the core mechanisms of life onset, and to optimize the safety and efficiency of in vitro reproductive technologies. This work focuses on meiotic progression and pre-implantation development in mammals, underlining the importance of fundamental molecules stored during oocyte growth and selectively used during early embryogenic stages. Taking into account the species-specific behaviour of these pivotal molecules, this review describes the advantages of using large domestic animals for research in the reproductive field and proposes large domestic animals as models to improve human ART.

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Section: Maternal Effect Genes: Expression Patterns In Animal Modelssupporting

confidence: 62%

Unveiling mRNA Changes During Meiotic Progression and Pre-Implantation Development: Help from Large Animal Models

Ledda

Bebbere²,

Ariu³

et al. 2012

CPD

Self Cite

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“…These genes, which are called maternal effect genes and which include NLRP5, Zygotic Arrest 1 (Zar1) [8,9], Stella [10,11] and Nucleoplasmin 2 (Npm2) [12], are all required for normal embryonic development beyond the 1-and 2-cell stage in mice [13,14]. Knock-out of these genes leads to incapacity of the embryo to develop beyond the 2-cell stage [15].…”

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confidence: 99%

Characterization of Maternal Antigen That Embryos Require (MATER/NLRP5) Gene and Protein in Pig Somatic Tissues and Germ Cells

Pisani

Ramelli

Lazzari

et al. 2010

Journal of Reproduction and Development

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Abstract. Maternal effect genes produce mRNA or proteins that accumulate in the egg during oogenesis and control the developmental program until embryonic genome activation takes place. NLRP5 (NLR family, Pyrin domain containing 5), also called MATER (Maternal Antigen That Embryos Require) is one of the genes required for normal early embryonic development, although its precise function remains to be elucidated. The aim of the present study was to analyze the NLRP5 gene expression pattern and protein distribution in somatic tissues and germ cells in the pig. Reverse transcription was performed on mRNA from germinal vescicle (GV) oocytes and total RNA from spermatozoa and tissues from different organs. The transcript for NLRP5 gene was identified only in ovaries and oocytes. The presence of NLRP5 protein was detected only in ovaries by western blot analysis and immunohistochemistry. Key words: Maternal effect genes, NLR family, Pyrin domain containing 5 (NLRP5), Oocytes, Pig (J. Reprod. Dev. 56: [41][42][43][44][45][46][47][48] 2010) ver the past few years, a number of mammalian genes predominantly or exclusively expressed in germ cells have been discovered. Functional studies have reported the essential role of these genes during gametogenesis, folliculogenesis and early embryonic development [1][2][3][4][5][6][7]. NLRP5 (NLR, pyrin domain containing5), also called MATER (Maternal Antigen That Embryos Require), is one of these genes. However, its precise function remains to be elucidated, although the transcription patterns may suggest a role in embryonic genome activation.Maternal mRNAs accumulated in the oocyte have a crucial role for successful embryo development before activation of the embryonic genome (EGA) [3], and some of them are also required for embryo development after EGA and implantation. These genes, which are called maternal effect genes and which include NLRP5, Zygotic Arrest 1 (Zar1) [8,9], Stella [10,11] and Nucleoplasmin 2 (Npm2) [12], are all required for normal embryonic development beyond the 1-and 2-cell stage in mice [13,14]. Knock-out of these genes leads to incapacity of the embryo to develop beyond the 2-cell stage [15].Developmental block in embryos produced in vitro remains the main problem in assisted reproduction of farm animals, particularly in cattle and pigs. An increasing amount of data indicates that the embryonic genome activation is crucial for the success of preimplantation embryo development [16]. To ensure maternal-embryo transition (MET) of gene expression, oocytes have to reach a sufficiently advanced level of developmental competence during differentiation and maturation [17][18][19]. Maternally expressed genes are implicated in this process, and some of them are associated with developmental competence [20,21]. In domestic species, MET occurs at the 4-16-cell stage [22], a stage in which enough biological material is available to study the fate of maternal mRNAs and their action in regulating embryo cleavages. Studying these genes in farm animals provides a model ...

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“…The SCMC is localized in the subcortical region of mouse cleavage-stage embryos, although excluded from regions of cell-cell contact (5). The SCMC transcripts expression pattern has been previously reported in ovine oocyte and pre-implantation embryos (12,30). Our recent study on bovine oocyte showed Filia protein distribution through the cytoplasm of antral follicles (14).…”

Section: Discussionmentioning

confidence: 56%

Expressional and Bioinformatic Analysis of Bovine Filia/Ecat1/Khdc3l Gene: A Comparison with Ovine Species

Zahmatkesh

Mahyari

Joupari

et al. 2016

Animal Biotechnology

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Maternal effect genes have highly impressive effects on pre-implantation development. Filia/Ecat1/Khdc3l is a maternal effect gene found in mouse oocytes and embryos, loss of which causes a 50% decrease in fertility. In the present study, we investigated Filia mRNA expression in bovine oviduct, 30- to 40-day fetus, liver, heart, lung, and oocytes (as a positive control), by RT-PCR and detected it only in oocytes. A 443 bp fragment was amplified only in oocytes and was sequenced as a part of bovine predicted Filia mRNA. We analyzed bovine and ovine Filia N-terminal peptide sequence in PHYRE2, and a KH domain was predicted. Protein alignment using ClustalW indicated a highly identical N-terminal extention between the 2 species. Immunohistochemical analysis using anti-bovine Filia antibody showed the expression of Filia protein in the zone surrounding the nuclear membrane, and in the subcortex of ovine oocytes of primary and antral follicles. However, in the bovine, Filia has been found through the oocyte cytoplasm of antral follicles, and here it is further confirmed in the primary follicles. Our data suggests a difference in Filia expression pattern between cow and sheep, although the sequence is highly conserved.

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Expression pattern of zygote arrest 1 (ZAR1), maternal antigen that embryo requires (MATER), growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) genes in ovine oocytes and in vitro-produced preimplantation embryos

Cited by 39 publications

References 43 publications

Unveiling mRNA Changes During Meiotic Progression and Pre-Implantation Development: Help from Large Animal Models

Unveiling mRNA Changes During Meiotic Progression and Pre-Implantation Development: Help from Large Animal Models

Characterization of Maternal Antigen That Embryos Require (MATER/NLRP5) Gene and Protein in Pig Somatic Tissues and Germ Cells

Expressional and Bioinformatic Analysis of Bovine Filia/Ecat1/Khdc3l Gene: A Comparison with Ovine Species

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