Expression patterns and colocalization of two sensory neurone membrane proteins in <i>Ectropis obliqua</i> Prout, a geometrid moth pest that uses Type‐II sex pheromones

Sun, Lina; Wang, Q.; Zhang, Y.; Yan, Ying; Guo, Huijuan; Xiao, Qiang

doi:10.1111/imb.12555

Cited by 12 publications

(8 citation statements)

References 45 publications

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“…SNMP was first identified from Lepidoptera and exhibited high expression in pheromone receptor neurons of the antennae (Rogers et al, 2001). Since then SNMP2 has been found that is expressed in antennae but also other tissues and is thought to be involved in multiple olfactory roles (Sun et al, 2018). The SNMP2 gene was also found in the transcriptome of H. zea PG and tarsi.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional comparison between pheromone gland-ovipositor and tarsi in the corn earworm moth Helicoverpa zea

Dou

Liu

Ahn

et al. 2019

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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The corn earworm, Helicoverpa zea, utilizes (Z)-11-hexadecenal as the major sex pheromone component. The saturated fatty acid derivative hexadecanal is also found in the pheromone gland and recently a large amount (0.5-1.5 μg) was found in male tarsi with lower amounts (0.05-0.5 μg) in female tarsi. In this study, we compared the transcriptome between female pheromone glands (including the ovipositor) and female and male tarsi to identify differences between these tissues, particularly the genes involved in sex pheromone biosynthesis and chemosensation. We found transcripts encoding 9 fatty acyl-CoA desaturases, 20 fatty acyl-CoA reductases, 8 alcohol oxidases, some G protein-coupled receptors and many transcripts involved in signal transduction and pheromone transportation. Also we found gustatory and olfactory receptors associated with the tarsi and ovipositor. Differential expression analysis showed that there were many genes differentially expressed between tissues, including the candidate desaturases, fatty acyl-CoA reductases, and alcohol oxidases. We discuss how some of these genes produce proteins that could be involved in the biosynthesis of hexadecanal in tarsi and (Z)-11-hexadecenal in the pheromone gland and the possible role of proteins in chemosensation of the tarsi and ovipositor.

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Section: Discussionmentioning

confidence: 99%

Transcriptional comparison between pheromone gland-ovipositor and tarsi in the corn earworm moth Helicoverpa zea

Dou

Liu

Ahn

et al. 2019

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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“…SNMPs are divided into SNMP1 and SNMP2 subgroups (Forstner et al, 2008). Notably, SNMPs are known to participate in pheromone detection (Jin, Ha & Smith, 2008;Pregitzer et al, 2014;Sun et al, 2019), although the molecular mechanisms of their involvement in chemoreception are unknown.…”

Section: Introductionmentioning

confidence: 99%

Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Yang¹,

Dong²,

Sun³

et al. 2020

PeerJ

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Insect olfaction and vision play important roles in survival and reproduction. Diurnal butterflies mainly rely on visual cues whereas nocturnal moths rely on olfactory signals to locate external resources. Histia rhodope Cramer (Lepidoptera: Zygaenidae) is an important pest of the landscape tree Bischofia polycarpa in China and other Southeast Asian regions. As a diurnal moth, H. rhodope represents a suitable model for studying the evolutionary shift from olfactory to visual communication. However, only a few chemosensory soluble proteins have been characterized and information on H. rhodope chemoreceptor genes is currently lacking. In this study, we identified 45 odorant receptors (ORs), nine ionotropic receptors (IRs), eight gustatory receptors (GRs) and two sensory neuron membrane proteins (SNMPs) from our previously acquired H. rhodope antennal transcriptomic data. The number of chemoreceptors of H. rhodope was less compared with that found in many nocturnal moths. Some specific chemoreceptors such as OR co-receptor (ORco), ionotropic receptors co-receptor, CO2 receptors, sugar receptors and bitter receptors were predicted by phylogenetic analysis. Notably, two candidate pheromone receptors (PRs) were identified within a novel PR lineage. qRT-PCR results showed that almost all tested genes (22/24) were predominantly expressed in antennae, indicating that they may be important in olfactory function. Among these antennae-enriched genes, six ORs, five IRs and two GRs displayed female-biased expression, while two ORs displayed male-biased expression. Additionally, HrhoIR75q.2 and HrhoGR67 were more highly expressed in heads and legs. This study enriches the olfactory gene inventory of H. rhodope and provides the foundation for further research of the chemoreception mechanism in diurnal moths.

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“…Two SNMPs have distinct 1. expression patterns in the antennal sensilla of Ectropis obliqua (Sun et al, 2019b), suggesting a functional diversification between the two genes. In D. melanogaster, H. virescens, and B. mori, SNMP1s play critical roles in pheromone signaling (Jin et al, 2008;Pregitzer et al, 2014;Zhang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Previous research on Heliothis virescens and Antheraea polyphemus demonstrated that SNMP1 s are co-expressed with PR s in the pheromone-responsive neurons, whereas SNMP2 s are expressed in the supporting cells around the neurons ( Forstner et al, 2008 ). Two SNMP s have distinct expression patterns in the antennal sensilla of Ectropis obliqua ( Sun et al, 2019b ), suggesting a functional diversification between the two genes. In D. melanogaster , H. virescens , and B. mori , SNMP1s play critical roles in pheromone signaling ( Jin et al, 2008 ; Pregitzer et al, 2014 ; Zhang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Insect SNMPs have homology with the human fatty acid transporter CD36 and are divided into two subfamilies: SNMP1 and SNMP2 ( Vogt et al, 2009 ). SNMP1s are co-expressed with pheromone receptors (PRs) accumulating on the membrane of pheromone-sensitive OSNs, whereas SNMP2s are expressed in the cells surrounding the pheromone-sensitive OSNs ( Forstner et al, 2008 ; Sun et al, 2019b ). SNMPs may have the ability to transfer lipophilic sex pheromones to ORs; in fruit fly and several moth species, SNMP1s are crucial for the detection of pheromones ( Jin et al, 2008 ; Zhang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Identification of Olfactory Genes From the Greater Wax Moth by Antennal Transcriptome Analysis

et al. 2021

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The olfactory system is used by insects to find hosts, mates, and oviposition sites. Insects have different types of olfactory proteins, including odorant-binding proteins (OBPs), chemosensory proteins (CSPs), odorant receptors (ORs), ionotropic receptors (IRs), and sensory neuron membrane proteins (SNMPs) to perceive chemical cues from the environment. The greater wax moth, Galleria mellonella, is an important lepidopteran pest of apiculture. However, the molecular mechanism underlying odorant perception in this species is unclear. In this study, we performed transcriptome sequencing of G. mellonella antennae to identify genes involved in olfaction. A total of 42,544 unigenes were obtained by assembling the transcriptome. Functional classification of these unigenes was determined by searching against the Gene Ontology (GO), eukaryotic orthologous groups (KOG), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. We identified a total of 102 olfactory-related genes: 21 OBPs, 18 CSPs, 43 ORs, 18 IRs, and 2 SNMPs. Results from BLASTX best hit and phylogenetic analyses showed that most of the genes had a close relationship with orthologs from other Lepidoptera species. A large number of OBPs and CSPs were tandemly arrayed in the genomic scaffolds and formed gene clusters. Reverse transcription-quantitative PCR results showed that GmelOBP19 and GmelOR47 are mainly expressed in male antennae. This work provides a transcriptome resource for olfactory genes in G. mellonella, and the findings pave the way for studying the function of these genes.

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Expression patterns and colocalization of two sensory neurone membrane proteins in Ectropis obliqua Prout, a geometrid moth pest that uses Type‐II sex pheromones

Cited by 12 publications

References 45 publications

Transcriptional comparison between pheromone gland-ovipositor and tarsi in the corn earworm moth Helicoverpa zea

Transcriptional comparison between pheromone gland-ovipositor and tarsi in the corn earworm moth Helicoverpa zea

Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Identification of Olfactory Genes From the Greater Wax Moth by Antennal Transcriptome Analysis

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