Expression patterns and oestradiol regulation of growth differentiation factor 9 in Schizothorax prenanti

Yan, Taiming; Zhang, Songpei; Zhang, Qian; Deng, Faqiang; Cai, Yueping; He, Jiayang; He, Liang; Luo, Jie; Yang, Deying; He, Zhi

doi:10.1016/j.cbpb.2020.110470

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…Schizothorax prenanti , which belongs to the subfamily Schizothoracinae of the Cyprinidae family, is mainly distributed in regions adjacent to the Qinghai-Tibet Plateau (QTP) and an endemic fish species with great economic importance in aquaculture in Western China [ 25 ]. Our previous report showed that GDF9 plays an important role in folliculogenesis and spermatogenesis in S. prenanti [ 26 ], but the downstream signal transduction mechanism remains unclear. Because ALK5 and BMPR2 are important and necessary for signal transduction, their regulatory roles in the gonadal development process of S. prenanti should be unraveled.…”

Section: Introductionmentioning

confidence: 99%

Estradiol Upregulates the Expression of the TGF-β Receptors ALK5 and BMPR2 during the Gonadal Development of Schizothorax prenanti

Yan

Zhang

Cai

et al. 2021

Animals

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TGF-β receptors play important roles in mediating TGF-β signals during gonadal development. To identify the functions of TGF-β receptors, including the type I receptor (activin receptor-like kinase 5, ALK5) and type II receptor (bone morphogenetic protein receptor 2, BMPR2), during the gonadal development of S. prenanti, the full-length cDNA sequences of ALK5 and BMPR2 were isolated and characterized. Their expression patterns in developing gonads and in the gonads of exogenous estradiol (E2) -fed fish were analyzed. The cDNAs of ALK5 and BMPR2 were 1925 bp and 3704 bp in length and encoded 501 and 1070 amino acid residues, respectively. ALK5 and BMPR2 were mostly expressed in gonads, particularly in cortical alveoli stage ovaries and mid-spermatogenic stage testes; however, the overall level of BMPR2 mRNA was higher than that of ALK5 during gonadal development. Furthermore, immunohistochemical signals of ALK5 and BMPR2 were mostly detected at chromatin nucleolar oocytes and perinuclear oocytes in ovaries and at spermatocytes and spermatogonia in testes. Exogenous E2 induces the gonadal expression of ALK5 and BMPR2, and BMPR2 is more responsive to E2 than ALK5. These results suggest that ALK5 and BMPR2 might play a potentially vital role in both folliculogenesis and spermatogenesis in S. prenanti.

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Section: Introductionmentioning

confidence: 99%

Estradiol Upregulates the Expression of the TGF-β Receptors ALK5 and BMPR2 during the Gonadal Development of Schizothorax prenanti

Yan

Zhang

Cai

et al. 2021

Animals

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“…Notably, GDF9, a member of the transforming growth factor beta (TGFβ) superfamily, was first found as an oocyte-specific growth factor in vertebrates (McPherron and Lee 1993 ; McGrath et al 1995 ) and is known as one of the control factors of steroid hormones: down-regulating estradiol and progesterone while up-regulating androgen (Solovyeva et al 2000 ; Vitt et al 2000 ; Spicer et al 2006 ; Orisaka et al 2009 ). In addition, GDF9 also acts on germ or somatic cells and controls spermatogenesis in vivo (He et al 2012 ; Guo et al 2013 ; Yan et al 2020 ). In a previous study, we found that the precocious trait of the male tiger pufferfish was characterized by high levels of plasma estradiol-17β (E2) prior to the initiation of testicular development (Yoshikawa et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic Dissection of a Precocious Phenotype in Male Tiger Pufferfish (Takifugu rubripes) using Genotyping by Random Amplicon Sequencing, Direct (GRAS-Di)

et al. 2021

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The novel non-targeted PCR-based genotyping system, namely Genotyping by Random Amplicon Sequencing, Direct (GRAS-Di), is characterized by the simplicity in library construction and robustness against DNA degradation and is expected to facilitate advancements in genetics, in both basic and applied sciences. In this study, we tested the utility of GRAS-Di for genetic analysis in a cultured population of the tiger pufferfish Takifugu rubripes. The genetic analyses included family structure analysis, genetic map construction, and quantitative trait locus (QTL) analysis for the male precocious phenotype using a population consisting of four full-sib families derived from a genetically precocious line. An average of 4.7 million raw reads were obtained from 198 fish. Trimmed reads were mapped onto a Fugu reference genome for genotyping, and 21,938 putative single-nucleotide polymorphisms (SNPs) were obtained. These 22 K SNPs accurately resolved the sibship and parent–offspring pairs. A fine-scale linkage map (total size: 1,949 cM; average interval: 1.75 cM) was constructed from 1,423 effective SNPs, for which the allele inheritance patterns were known. QTL analysis detected a significant locus for testes weight on Chr_14 and three suggestive loci on Chr_1, Chr_8, and Chr_19. The significant QTL was shared by body length and body weight. The effect of each QTL was small (phenotypic variation explained, PVE: 3.1–5.9%), suggesting that the precociousness seen in the cultured pufferfish is polygenic. Taken together, these results indicate that GRAS-Di is a practical genotyping tool for aquaculture species and applicable for molecular breeding programs, such as marker-assisted selection and genomic selection.

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“…Compared with other non-gonadal tissues, the ovary showed more expression of the GDF9 mRNA. Although the GDF9 mRNA is expressed at high levels in the ovary, its expression shows a stage-specific pattern during ovarian development, and it is expressed at greater levels during the start of primary oocytes from oogonium or vitellogenesis during oocyte maturation ( Yan et al., 2020 ). It was also reported that lack of GDF9 expression in mice affected follicle growth and led to infertility.…”

Section: Methodsmentioning

confidence: 99%

Chicken ovarian follicles morphology and growth differentiation factor 9 gene expression in chicken ovarian follicles: review

Hlokoe

Tyasi

Gunya

2022

Heliyon

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Ovary follicular development is a progressive system from the beginning of small cortical follicles to the ovulation of hierarchical follicles. The review was conducted to provide information on the indigenous chickens commonly used for egg production, chicken ovarian follicles morphology and expression of growth differentiation factor 9 (GDF9) gene in ovarian follicles and its relationship with egg production. The research databases used in the study include google scholar, Science Direct, PubMed, JSTOR and Cambridge Core. Google, Yahoo and Baidu search engines were used to search the information. In this study, the papers selected for use were original research articles and reviews to ensure that the information used was from research results. Besides, only recent English papers, 2010-2021, were used. The keywords used to search for articles were chicken ovarian follicles, ovarian morphology and GDF9 gene expression. The documents showed that pre-hierarchical follicles include many small and large white follicles, which are about 2-5mm in diameter and 5 to 6 small yellow follicles (SYF) that are about 5-10mm in diameter. Preovulatory follicles are about five to six in number and above 10mm in diameter, with the sizes from F6 to F1, with F1 as the largest follicle. Further, the studies revealed that GDF9 gene mRNA is expressed in the highest concentration in small yellow follicles and other studies reported that the expression of GDF9 gene has been found in follicles of the primary to preovulatory stages in chickens. This review concludes that the GDF9 gene expression is mainly throughout follicular growth and it stimulates the proliferation of pre-hierarchical granulosa cells. The increased egg production in chickens depends on progressive developmental stages and the growth of ovarian follicles.

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Expression patterns and oestradiol regulation of growth differentiation factor 9 in Schizothorax prenanti

Cited by 4 publications

References 42 publications

Estradiol Upregulates the Expression of the TGF-β Receptors ALK5 and BMPR2 during the Gonadal Development of Schizothorax prenanti

Estradiol Upregulates the Expression of the TGF-β Receptors ALK5 and BMPR2 during the Gonadal Development of Schizothorax prenanti

Genetic Dissection of a Precocious Phenotype in Male Tiger Pufferfish (Takifugu rubripes) using Genotyping by Random Amplicon Sequencing, Direct (GRAS-Di)

Chicken ovarian follicles morphology and growth differentiation factor 9 gene expression in chicken ovarian follicles: review

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