Expression patterns and role of SDF-1/CXCR4 axis in boar spermatogonial stem cells

Park, Hyun Jung; Lee, Won Young; Kim, Jin‐Hoi; Park, Chankyu; Song, Hyuk

doi:10.1016/j.theriogenology.2018.03.008

Cited by 9 publications

(4 citation statements)

References 39 publications

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“…Previously, we described an important role of CXCR4 in mouse SSC maintenance [22]. Interestingly, recent studies reported that CXCR4 + cells contained enriched SSC activity in bull, boar and human testis [32, 43, 44] . Findings from the current study demonstrated that CXCR4 was only expressed in gonocytes and a small number of spermatogonia; we therefore proposed that CXCR4 can be used as a marker for SSC enrichment.…”

Section: Discussionmentioning

confidence: 99%

Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak

Wang

et al. 2019

J Animal Sci Biotechnol

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Background Spermatogenesis is a cellular differentiation process that includes three major events: mitosis of spermatogonia, meiosis of spermatocytes and spermiogenesis. Steady-state spermatogenesis relies on functions of spermatogonial stem cells (SSCs). Establishing and maintaining a foundational SSC pool is essential for continued spermatogenesis in mammals. Currently, our knowledge about SSC and spermatogenesis is severely limited in domestic animals. Results In the present study, we examined transcriptomes of testes from domestic yaks at four different stages (3, 5, 8 and 24 months of age) and attempted to identify genes that are associated with key developmental events of spermatogenesis. Histological analyses showed that the most advanced germ cells within seminiferous tubules of testes from 3, 5, 8 and 24 months old yaks were gonocytes, spermatogonia, spermatocytes and elongated spermatids, respectively. RNA-sequencing (RNA-seq) analyses revealed that 11904, 4381 and 2459 genes were differentially expressed during the gonocyte to spermatogonia transition, the mitosis to meiosis transition and the meiosis to post-meiosis transition. Further analyses identified a list of candidate genes than may regulate these important cellular processes. CXCR4 , a previously identified SSC niche factor in mouse, was one of the up-regulated genes in the 5 months old yak testis. Results of immunohistochemical staining confirmed that CXCR4 was exclusively expressed in gonocytes and a subpopulation of spermatogonia in the yak testis. Conclusions Together, these findings demonstrated histological changes of postnatal testis development in the domestic yak. During development of spermatogonial lineage, meiotic and haploid germ cells are supported by dynamic transcriptional regulation of gene expression. Our transcriptomic analyses provided a list of candidate genes that potentially play crucial roles in directing the establishment of SSC and spermatogenesis in yak. Electronic supplementary material The online version of this article (10.1186/s40104-019-0360-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak

Wang

et al. 2019

J Animal Sci Biotechnol

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“…[ 168 ] Stromal cell‐derived factor 1 α (SDF‐1 α ) is a chemokine that causes mesenchymal stem cells to migrate from specific stem cell niches to the site of injury. [ 169,170 ] An in vivo study demonstrated that SDF‐1 α promoted differentiation of dental pulp stem cells, which was followed by pulp regeneration. [ 171 ] The synergistic effect of BMP‐2 and SDF‐1 α on human stem cells from the apical papilla was demonstrated by incorporating the growth factor and the cytokine into an injectable hydrogel and implanted into a necrotic tooth.…”

Section: Gene and Protein Deliverymentioning

confidence: 99%

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

et al. 2021

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The oral cavity and oropharynx are complex environments that are susceptible to physical, chemical, and microbiological insults. They are also common sites for pathological and cancerous changes. The effectiveness of conventional locally-administered medications against diseases affecting these oral milieus may be compromised by constant salivary flow. For systemically-administered medications, drug resistance and adverse side-effects are issues that need to be resolved. New strategies for drug delivery have been investigated over the last decade to overcome these obstacles. Synthesis of nanoparticle-containing agents that promote healing represents a quantum leap in ensuring safe, efficient drug delivery to the affected tissues. Micro/nanoencapsulants with unique structures and properties function as more favorable drug-release platforms than conventional treatment approaches. The present review provides an overview of newly-developed nanocarriers and discusses their potential applications and limitations in various fields of dentistry and oral medicine.

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“…VECs migration and proliferation are inclined to vascular functions like relaxation, patency, and signal molecule release (e.g., nitric oxide and prostatic cyclin), and so forth (Laiva, Raftery, Keogh, & O'Brien, ; Li et al, ). Stromal cell‐derived factor‐1 (SDF‐1) is a cytokine with small molecule weight, which affects homing of mesenchymal stem cells (MSCs) to the lesion location via SDF‐1/CXCR4 axis (Park, Lee, Kim, Park, & Song, ). Vascular endothelial growth factor (VEGF) is a specific protein which promotes the VECs migration and proliferation, and it is also the preferable factor on inducing angiogenesis in vivo (Hu et al, ).…”

Section: Introductionmentioning

confidence: 99%

An injectable scaffold based on temperature‐responsive hydrogel and factor‐loaded nanoparticles for application in vascularization in tissue engineering

Zhao

et al. 2019

J Biomedical Materials Res

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Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration. A biomedical application requires the selective release of multiple factors which will guide the synergy of the cells. Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release. Series of in vitro and in vivo experiments demonstrate that our composited system can accurately guide MSCs and ECs for vascularization. In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).

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Expression patterns and role of SDF-1/CXCR4 axis in boar spermatogonial stem cells

Cited by 9 publications

References 39 publications

Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak

Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

An injectable scaffold based on temperature‐responsive hydrogel and factor‐loaded nanoparticles for application in vascularization in tissue engineering

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