Expression Patterns of Extracellular Matrix Components in Native and Cultured Normal Human Thyroid Tissue and in Human Toxic Adenoma Tissue

Burgi-Saville, M. E.; Gerber, H; Peter, HJ; Paulsson, Mats; Aeschlimann, Daniel; Glaser, Christine; Kaempf, Jacqueline; Ruchti, C; Sidiropoulos, I.; Bürgi, U

doi:10.1089/thy.1997.7.347

Cited by 15 publications

(12 citation statements)

References 16 publications

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“…Synthesis of laminin, CoIV and fibronectin has been demonstrated in the thyroid epithelial cell line FRTL5 (Ambesi-Impiombato et al 1980, Ambesi-Impiombato & Perrild 1989 and also the deposition of the extracellular material at the basolateral surface of the cells and its organization into a BM (Garbi et al 1988). We have recently demonstrated the maintenance of an ECM containing fibronectin, CoIV, laminin and perlecan both in FRTL5 cells and in primary cultures of rat thyroid cells (Bürgi-Saville et al 1998) and in primary cultures of normal human thyroid tissue, also in alginate beads (Bürgi-Saville et al 1997).…”

Section: Introductionmentioning

confidence: 73%

“…The sections were incubated overnight at room temperature with specific rabbit polyclonal primary antibodies to mouse laminin (Sigma, dilution 1:50), human fibronectin (Dako, Zug, Switzerland, dilution 1:250), mouse perlecan (kindly provided by Prof. J Hassell, University of Pittsburgh, PA, USA, dilution 1:100) and mouse CoIV (Becton Dickinson, Basel, Switzerland, dilution 1:250) (Studer et al 1992, Bürgi-Saville et al 1997. MIB-1, a monoclonal mouse antibody to the Ki-67 proliferationassociated antigen (Dianova, Hamburg, Germany, dilution 1:50), was used as a cell proliferation marker.…”

Section: Immunohistochemical Methodsmentioning

confidence: 99%

“…Alginate beads were prepared as described (Häuselmann et al 1994, Bürgi-Saville et al 1997. In brief, 6 10 6 cells were suspended in 6 ml of a solution of 1·2% alginate (sodium salt, Sigma) in 0·9% NaCl (final concentration 10 6 cells/ml alginate).…”

Section: Alginate Culture Techniquementioning

confidence: 99%

“…In order to study in vitro thyroid differentiation, function and proliferation, various cell culture systems have been tested: monolayers (Ambesi-Impiombato et al 1980, Ambesi-Impiombato & Perrild 1989, Derwahl et al 1990, Gerber et al 1991a,b, Peter et al 1991b, Dumont et al 1992, three-dimensional systems using collagen gels (Yang et al 1979, Garbi & Wollman 1982, Garbi et al 1984, Peter et al 1987, 1991a-c, Toda & Sugihara 1990, Toda et al 1993, agarose gels (Kimura et al 1980, Benya & Shaffer 1982, Aydelotte et al 1986a,b, Bruckner et al 1989) and alginate beads (Kupchick et al 1983, Guo et al 1989, Häuselmann et al 1994, Tognella et al 1995, Glaser et al 1996, Bürgi-Saville et al 1997, 1998. Alginate has several advantages in comparison with the other systems.…”

Section: Introductionmentioning

confidence: 99%

“…So far, much less but nevertheless fascinating research has been done on the structure and metabolism of ECM of thyroid tissue (Garbi & Wollman 1982, Alquier et al 1983, Garbi et al 1984, Wadeleux et al 1985, Demeure et al 1992, Prabakaran et al 1993, Vitale et al 1993, 1994, Andre et al 1994, Lemansky et al 1994, Tognella et al 1995, Arvan et al 1997, Bürgi-Saville et al 1997, 1998. Ultrastructural studies indicate that BM formation or maintenance can be achieved in cultures of thyroid cells (Alquier et al 1983, Garbi & Wollman 1982, Garbi et al 1988.…”

Section: Introductionmentioning

confidence: 99%

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Follicle-forming cat thyroid cell lines synthesizing extracellular matrix and basal membrane components: a new tool for the study of thyroidal morphogenesis

Tognella

Marti²,

Peter³

et al. 1999

Journal of Endocrinology

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Interactions between follicular epithelial cells and extracellular matrix (ECM) are supposed to play an important role in the development and maintenance of thyroid tissue architecture. In the present study we have therefore investigated the synthesis of ECM components by a feline thyroid cell line which is able to form follicle-like structures in vitro, and also in v-ras-transfected and controltransfected sublines. Transfections were performed by lipofection with pZSR (viral Harvey ras gene; neo) and pSV2-neo (control, neo only) plasmids. We have adapted a semisolid culture system composed exclusively of polymerized alginate and therefore devoid of ECM components. Feline cells embedded in alginate gels as single cells and cultured for up to 90 days formed cell clusters within 10 days. Follicle-like structures were formed in the original cell lines and also in the v-ras-and controltransfected cells. Differences in proliferation rates were observed, the v-ras-transfected cells growing up to two to three times faster than the non-transfected cells. Immunostaining was done using rabbit first antibodies directed against mouse collagen IV, human fibronectin, laminin (tumor Engelbreth-Holm-Swarm laminin), perlecan and other ECM components. For comparison, immunostaining was also performed on cryosections of nodular goiters of six hyperthyroid cats. The cell lines and their transfected clones stained strongly positive for collagen IV and fibronectin, and positively but less strongly for laminin and perlecan. The cat goiter tissue stained positively for collagen IV, laminin, perlecan, and fibronectin, and positive staining for S-laminin (containing the 2-chain) was seen in blood vessel walls in this tissue.In conclusion, cat cell lines grow three-dimensionally in alginate beads over several weeks, they form follicle-like structures and express the same ECM components as the native cat goiter tissue. Transfection with v-ras does increase proliferation rate, but does not fundamentally alter formation of follicle-like structures and ECM expression. Alginate gel culture is a promising new tool for the study of follicular morphogenesis, polarity, the expression pattern of ECM components and of the interaction between thyrocytes and ECM. It avoids interference caused by gels composed of ECM components.

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Section: Introductionmentioning

confidence: 73%

Section: Immunohistochemical Methodsmentioning

confidence: 99%

Section: Alginate Culture Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Follicle-forming cat thyroid cell lines synthesizing extracellular matrix and basal membrane components: a new tool for the study of thyroidal morphogenesis

Tognella

Marti²,

Peter³

et al. 1999

Journal of Endocrinology

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Anatomy and Development of the Thyroid

Felice¹,

Lauro²

2010

Endocrinology

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The follicular thyroid cell line PCCL3 responds differently to laminin and to polylaminin, a polymer of laminin assembled in acidic pH

Palmero

Miranda-Alves

Barroso

et al. 2013

Molecular and Cellular Endocrinology

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The extracellular-matrix protein laminin forms polymers both in vivo and in vitro. Acidification of pH leads to the formation of an artificial polymer with biomimetic properties, named polylaminin (polyLM). Follicle cells in the thyroid are in close contact with laminin, but their response to this important extracellular signal is still poorly understood. PCCL3 thyroid follicular cells cultured on glass, on regular laminin (LM) or on laminin previously polymerized in acidic pH (polyLM) showed different cell morphologies and propensities to proliferate, as well as differences in the organization of their actin cytoskeleton. On polyLM, cells displayed a typical epithelial morphology and radially organized actin fibers; whereas on LM, they spread irregularly on the substrate, lost cell contacts, and developed thick actin fibers extending through the entire cytoplasm. Iodide uptake decreased similarly in response to both laminin substrates, in comparison to glass. On both the LM and polyLM substrates, the expression of the sodium iodide symporter (NIS) decreased slightly but not significantly. NIS showed dotted immunostaining at the plasma membrane in the cells cultured on glass; on polyLM, NIS was observed mainly in the perinuclear region, and more diffusely throughout the cytoplasm on the LM substrate. Additionally, polyLM specifically favored the maintenance of cell polarity in culture. These findings indicate that PCCL3 cells can discriminate between LM and polyLM and that they respond to the latter by better preserving the phenotype observed in the thyroid tissue.

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Expression Patterns of Extracellular Matrix Components in Native and Cultured Normal Human Thyroid Tissue and in Human Toxic Adenoma Tissue

Cited by 15 publications

References 16 publications

Follicle-forming cat thyroid cell lines synthesizing extracellular matrix and basal membrane components: a new tool for the study of thyroidal morphogenesis

Follicle-forming cat thyroid cell lines synthesizing extracellular matrix and basal membrane components: a new tool for the study of thyroidal morphogenesis

Anatomy and Development of the Thyroid

The follicular thyroid cell line PCCL3 responds differently to laminin and to polylaminin, a polymer of laminin assembled in acidic pH

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