2014
DOI: 10.1016/j.gep.2014.01.006
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Expression patterns of gdnf and gfrα1 in rainbow trout testis

Abstract: In mice, glial cell line-derived neurotrophic factor (GDNF) is essential for normal spermatogenesis and in vitro culture of spermatogonial stem cells. In murine testes, GDNF acts as paracrine factor; Sertoli cells secrete it to a subset of spermatogonial cells expressing its receptor, GDNF family receptor α1 (GFRα1). However, in fish, it is unclear what types of cells express gdnf and gfrα1. In this study, we isolated the rainbow trout orthologues of these genes and analyzed their expression patterns during sp… Show more

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Cited by 42 publications
(24 citation statements)
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“…In the present study, we first confirm at a protein level that the GDNF receptor GFRα1 was expressed in undifferentiated spermatogonia in the dogfish testis until the first differentiating stages, in accordance with the mRNA expression pattern previously described [29]. An expression pattern in undifferentiated spermatogonia was also reported in many mammalian species such as mouse [7], primates [42], equids [8], domestic cat [43], collared peccary [10] or in fish such as the Nile tilapia [5] and a similar expression pattern extended to spermatocytes, but at a much lower level, was also observed in trout [44]. PLZF expression pattern appeared less restrictive than GFRa1 with an expression extended from undifferentiated spermatogonia to primary spermatocytes, which is consistent with the mRNA localization in germ cells of the dogfish [29].…”
Section: Discussionsupporting
confidence: 86%
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“…In the present study, we first confirm at a protein level that the GDNF receptor GFRα1 was expressed in undifferentiated spermatogonia in the dogfish testis until the first differentiating stages, in accordance with the mRNA expression pattern previously described [29]. An expression pattern in undifferentiated spermatogonia was also reported in many mammalian species such as mouse [7], primates [42], equids [8], domestic cat [43], collared peccary [10] or in fish such as the Nile tilapia [5] and a similar expression pattern extended to spermatocytes, but at a much lower level, was also observed in trout [44]. PLZF expression pattern appeared less restrictive than GFRa1 with an expression extended from undifferentiated spermatogonia to primary spermatocytes, which is consistent with the mRNA localization in germ cells of the dogfish [29].…”
Section: Discussionsupporting
confidence: 86%
“…Although rodent data describe GDNF expression in Sertoli cells [50,51], a recent work on a teleostean model, the rainbow trout, shows GDNF protein expression in spermatogonia [44]. In trout, gdnf transcripts are strongly expressed in A spermatogonia and decrease until primary spermatocytes, gdnf is no more detected in spermatids [44]. This expression pattern is similar to the Artemin protein expression observed in the present study in the dogfish.…”
Section: Discussionsupporting
confidence: 85%
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“…The sections were then stained with hematoxylin-eosin and were observed under a BX-51-34FL microscope (Olympus). All samples were processed via a method described previously [42].…”
Section: Detection Of Cre-mediated Excision Of the Loxp-flanked Transmentioning
confidence: 99%