Expression patterns of some genes involved in tanshinone biosynthesis in Salvia miltiorrhiza roots

Yang, Yongqiang; Hou, Shuang; Fan, Wei; Lu, Lilan; Hui, Nan; Wu, Xia; Wei, Jianhe

doi:10.1016/j.indcrop.2019.01.001

Cited by 12 publications

(5 citation statements)

References 40 publications

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“…Extracts from Salviae miltiorrhizae radix et rhizoma have also been documented to exert sedation effect ( Lobina et al, 2018 ). Tanshinones, the principal bioactive metabolites of Salviae miltiorrhizae radix et rhizoma ( Yang et al, 2019 ), demonstrate significant sedative-hypnotic effects ( Fang et al, 2010 ). In addition, animal studies demonstrated that schizandrin, a major metabolite of Schisandrae chinensis fructus, could pass through the blood-brain barrier and exhibited sedative and hypnotic bioactivity, potentially mediated by its modulation of the serotonergic system ( Zhang et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Commercial Chinese polyherbal preparation Zao Ren An Shen prescription for primary insomnia: a systematic review with meta-analysis and trial sequential analysis

Zhao,

Xu,

Kennedy

et al. 2024

Front. Pharmacol.

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Background: Natural products are widely used for primary insomnia (PI). This systematic review with trial sequential analysis (TSA) aimed to summarize evidence pertaining to the effectiveness and safety of Zao Ren An Shen (ZRAS) prescription, a commercial Chinese polyherbal preparation, for treating PI.Methods: Controlled clinical trials appraising ZRAS compared to controls or as an add-on treatment were systematically searched across seven databases until January 2024. Cochrane ROB 2.0 and ROBINS-I tools were adopted to determine risk of bias. Quality of evidence was assessed using the GRADE framework.Results: We analyzed 22 studies, involving 2,142 participants. The effect of ZRAS in reducing Pittsburgh Sleep Quality Index scores was found to be comparable to benzodiazepines [MD = 0.39, 95%CI (−0.12, 0.91), p = 0.13] and superior to Z-drugs [MD = −1.31, 95%CI (−2.37, −0.24), p = 0.02]. The addition of ZRAS to hypnotics more significantly reduced polysomnographically-recorded sleep onset latency [MD = −4.44 min, 95%CI (−7.98, −0.91), p = 0.01] and number of awakenings [MD = −0.89 times, 95%CI (−1.67, −0.10), p = 0.03], and increased total sleep time [MD = 40.72 min, 95%CI (25.14, 56.30), p < 0.01], with fewer adverse events than hypnotics alone. TSA validated the robustness of these quantitative synthesis results. However, the quality of evidence ranged from very low to low. The limited data available for follow-up did not support meta-synthesis.Conclusion: While ZRAS prescription shows promising effectiveness in treating PI, the overall quality of evidence is limited. Rigorously-designed randomized control trials are warranted to confirm the short-term efficacy of ZRAS and explore its medium-to-long-term efficacy.Systematic Review Registration: (https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=471497), identifier (CRD42023471497).

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Section: Discussionmentioning

confidence: 99%

Commercial Chinese polyherbal preparation Zao Ren An Shen prescription for primary insomnia: a systematic review with meta-analysis and trial sequential analysis

Zhao,

Xu,

Kennedy

et al. 2024

Front. Pharmacol.

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Section: Discussionmentioning

confidence: 99%

“…In the early stage of growth, tanshinones (TS I, TS IIA, and CTS) began to form and accumulate [81], but in the middle stage, tanshinone production tended to be limited because of the growth of S. miltiorrhiza. In the later stage, a large of amount tanshinones formed and accumulated [82]; therefore, it was implied that the demand for N differed at different stages of growth.…”

Section: Plos Onementioning

confidence: 99%

Integrative transcriptomic and metabolomic analyses unveil tanshinone biosynthesis in Salvia miltiorrhiza root under N starvation stress

Zhang

Yang

2022

PLoS ONE

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Salvia miltiorrhiza is a model plant for Chinese herbal medicine with significant pharmacologic effects due to its tanshinone components. Our previous study indicated that nitrogen starvation stress increased its tanshinone content. However, the molecular mechanism of this low nitrogen-induced tanshinone biosynthesis is still unclear. Thus, this study aimed to elucidate the molecular mechanism of tanshinone biosynthesis in S. miltiorrhiza under different N conditions [N-free (N0), low-N (Nl), and full-N (Nf, as control) conditions] by using transcriptome and metabolome analyses. Our results showed 3,437 and 2,274 differentially expressed unigenes between N0 and Nf as well as Nl and Nf root samples, respectively. N starvation (N0 and Nl) promoted the expression of the genes involved in the MVA and MEP pathway of tanshinone and terpenoid backbone biosynthesis. Gene ontology and KEGG analyses revealed that terpenoid backbone biosynthesis, hormone signal transduction, and phenylpropanoid biosynthesis were promoted under N starvation conditions, whereas starch and sucrose metabolisms, nitrogen and phosphorus metabolisms, as well as membrane development were inhibited. Furthermore, metabolome analysis showed that metabolite compounds and biosynthesis of secondary metabolites were upregulated. This study provided a novel insight into the molecular mechanisms of tanshinone production in S. miltiorrhiza in response to nitrogen stress.

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“…Many reference genes have been used to investigate gene expression in a number of plants, including Actin 31 , 32 , GAPDH 25 , Ubiquitin 34 , and 18S rRNA 26 . There is not one single stable and suitable gene for all experimental conditions, such as different tissues and development periods, as well as stress treatments 17 , 35 , 36 .…”

Section: Discussionmentioning

confidence: 99%

Selection and validation of reference genes for quantitative gene expression normalization in Taxus spp.

Zhang

Fan

Chen

et al. 2020

Sci Rep

Self Cite

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Quantitative real-time PCR (qRT-PCR) is commonly used to measure gene expression to further explore gene function, while suitable reference genes must be stably expressed under different experimental conditions to obtain accurate and reproducible data for relative quantification. Taxol or paclitaxel is an important anticancer compound mainly identified in Taxus spp. The molecular mechanism of the regulation of taxol biosynthesis is current research goal. However, in the case of Taxus spp., few reports were published on screening suitable reference genes as internal controls for qRT-PCR. Here, eight reference genes were selected as candidate reference genes for further study. Common statistical algorithms geNorm, NormFinder, BestKeeper, ΔCt, and RefFinder were used to analyze the data from samples collected from a cell line of Taxus × media under various experimental conditions and from tissues of Taxus chinensis var. mairei. The expression patterns of TcMYC under salicylic acid treatment differed significantly, with the best and worst reference genes in the cell line. This study screened out suitable reference genes (GAPDH1 and SAND) under different treatments and tissues for the accurate and reliable normalization of the qRT-PCR expression data of Taxus spp. At the same time, this study will aid future research on taxol biosynthesis-related genes expression in Taxus spp., and can also be directly used to other related species.

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Expression patterns of some genes involved in tanshinone biosynthesis in Salvia miltiorrhiza roots

Cited by 12 publications

References 40 publications

Commercial Chinese polyherbal preparation Zao Ren An Shen prescription for primary insomnia: a systematic review with meta-analysis and trial sequential analysis

Commercial Chinese polyherbal preparation Zao Ren An Shen prescription for primary insomnia: a systematic review with meta-analysis and trial sequential analysis

Integrative transcriptomic and metabolomic analyses unveil tanshinone biosynthesis in Salvia miltiorrhiza root under N starvation stress

Selection and validation of reference genes for quantitative gene expression normalization in Taxus spp.

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