Expression patterns of two pal genes of Pleurotus ostreatus across developmental stages and under heat stress

Hou, Ludan; Wang, Lining; Wu, Xiangli; Gao, Wei; Zhang, Jinxia; Huang, Chenyang

doi:10.1186/s12866-019-1594-4

Cited by 42 publications

(41 citation statements)

References 43 publications

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“…Using previously reported methods [ 39 ], we successfully constructed an RNAi- aox plasmid (Additional file 1 : Fig. S1) and established aox -transformed strains via Agrobacterium- mediated transformation.…”

Section: Resultsmentioning

confidence: 99%

“…The aox -JC-F/R primers (Additional file 1 : Table S1) were used to amplify the target fragment and thus detect whether the interference plasmid was successfully constructed. Finally, the RNAi- aox plasmid was transformed into P. ostreatus mycelia by A. tumefaciens GV3101 according to our previous transformation method [ 39 ]. The hyg fragment was amplified to screen the transformed strains, and RT-qPCR was performed to identify the strains with high interference for further experiments.…”

Section: Methodsmentioning

confidence: 99%

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Alternative oxidase gene induced by nitric oxide is involved in the regulation of ROS and enhances the resistance of Pleurotus ostreatus to heat stress

et al. 2021

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Background In China, during the cultivation process of Pleurotus ostreatus, the yield and quality of fruiting bodies are easily affected by high temperatures in summer. Nitric oxide (NO) plays an important regulatory role in the response to abiotic stress, and previous studies have found that NO can induce alternative oxidase (aox) experssion in response to heat stress (HS) by regulating aconitase. However, the regulatory pathway of NO is complex, and the function and regulation of the aox gene in the response to HS remain unclear. Results In this study, we found that NO affected nicotinamide adenine dinucleotide (NADH) and adenosine triphosphate (ATP) levels, reduced hydrogen peroxide (H2O2) and superoxide anion (O2−) contents, and slowed O2− production. Further RNA-Seq results showed that NO regulated the oxidation-reduction process and oxidoreductase activity, affected the cellular respiration pathway and activated aox gene expression. The function of aox was determined by constructing overexpression (OE) and RNA interference (RNAi) strains. The results showed that the OE-aox strains exhibited obviously improved growth recovery after exposure to HS. During exposure to HS, the OE-aox strains exhibited reduced levels of NADH, the product of the tricarboxylic acid (TCA) cycle, and decreased synthesis of ATP, which reduced the production and accumulation of reactive oxygen species (ROS), whereas the RNAi-aox strains exhibited the opposite result. In addition, aox mediated the expression of antioxidant enzyme genes in the mycelia of P. ostreatus under HS through the retrograde signaling pathway. Conclusions This study shows that the expression of the aox gene in P. ostreatus mycelia can be induced by NO under HS, that it regulates the TCA cycle and cell respiration to reduce the production of ROS, and that it can mediate the retrograde signaling pathway involved in the mycelial response to HS. Graphical abstract

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Alternative oxidase gene induced by nitric oxide is involved in the regulation of ROS and enhances the resistance of Pleurotus ostreatus to heat stress

et al. 2021

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“…Pleurotus ostreatus, a basidiomycete fungi, has effective regulatory protein expression that enhances its adaptation to stress triggers. 3 In our previous studies, we reported that the oyster fungi Pleurotus djamor exhibit trains of electrical potential spikes similar to action potential spikes. 4−7 Our initial assumption was that spike trains might reflect increasing mycelium propagation in the substrate, nutrient and metabolite transport, and communication processes within the mycelium network.…”

Section: Introductionmentioning

confidence: 94%

“…They have extracellular and intracellular sensing mechanisms, as well as protein receptors that enable them to detect and respond to a variety of signals. Pleurotus ostreatus, a basidiomycete fungi, has effective regulatory protein expression that enhances its adaptation to stress triggers …”

Section: Introductionmentioning

confidence: 99%

Stimulating Fungi Pleurotus ostreatus with Hydrocortisone

Dehshibi

Chiolerio

Nikolaidou

et al. 2021

ACS Biomater. Sci. Eng.

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Fungi cells can sense extracellular signals via reception, transduction, and response mechanisms, allowing them to communicate with their host and adapt to their environment. They feature effective regulatory protein expressions that enhance and regulate their response and adaptation to various triggers such as stress, hormones, physical stimuli such as light, and host factors. In our recent studies, we have shown that Pleurotus oyster fungi generate electrical potential impulses in the form of spike events in response to their exposure to environmental, mechanical, and chemical triggers, suggesting that the nature of stimuli may be deduced from the fungal electrical responses. In this study, we explored the communication protocols of fungi as reporters of human chemical secretions such as hormones, addressing whether fungi can sense human signals. We exposed Pleurotus oyster fungi to hydrocortisone, which was directly applied to the surface of a fungal-colonized hemp shavings substrate, and recorded the electrical activity of the fungi. Hydrocortisone is a medicinal hormone replacement that is similar to the natural stress hormone cortisol. Changes in cortisol levels released by the body indicate the presence of disease and can have a detrimental effect on physiological process regulation. The response of fungi to hydrocortisone was also explored further using X-rays to reveal changes in the fungi tissue, where receiving hydrocortisone by the substrate can inhibit the flow of calcium and, as a result, reduce its physiological changes. This research could open the way for future studies on adaptive fungal wearables capable of detecting human physiological states and biosensors built of living fungi.

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“…ROS scavenging enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) play an essential role in regulating ROS levels and the extent of oxidative damage (Niu et al, 2018). The phenylpropanoid pathway is another defense response in higher plants (Irisarri et al, 2016;Hou et al, 2019). Phenylalanine ammonia-lyase (PAL) is the first enzyme that participates in the formation of a series of structurally different and defensive lignin and phenolic compounds (Vogt, 2010;Wang et al, 2013;Kamalipourazad et al, 2016;Han et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Differential Responses of Cucurbita pepo to Podosphaera xanthii Reveal the Mechanism of Powdery Mildew Disease Resistance in Pumpkin

Zhang

Liu

et al. 2021

Front. Plant Sci.

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Powdery mildew is one of the most destructive diseases and the major cause to the production losses of cucurbit worldwide. A number of strategies have been developed and applied to discover suitable and safer methods to manage the occurrence of powdery mildew disease in pumpkins (Cucurbita pepo L.), but information is limited in screening tolerant germplasms and exploring their mechanisms in preventing the disease occurrence at physiological, biochemical, and molecular levels. Therefore, we investigated the responses of two commercial pumpkin cultivars to Podosphaera xanthii infection. Compared with mock-inoculated seedlings, few small and sparse powdery areas were observed on the leaves of the Sixing F1 cultivar on the 13th day after inoculation with P. xanthii, whereas a large number of diseased powdery areas and a layer of white powdery mildew were observed on the surface of Jin12 F1 leaves. The inoculation duration (7, 9, 11, and 13 days) significantly and continuously increased the disease incidence and index of pumpkin seedlings. The contents of H2O2, MDA, lignin, and total phenolics in the leaves of Sixing F1 and Jin12 F1 cultivars were markedly increased after inoculation with P. xanthii. However, the Sixing F1 cultivar exhibited much less reactive oxygen species (ROS) accumulation, a lower rate of lipid peroxidation, and a higher level of lignin and total phenolics contents after inoculation than the Jin12 F1 cultivar. Compared with untreated control pumpkin seedlings, significantly higher activities and gene expressions of the phenylpropanoids pathway enzymes (PAL and PPO), ROS scavenging defense enzymes (SOD, CAT, POD, and APX), and other salicylic acid (SA) signaling pathway marker genes were observed in the leaves of both cultivars after P. xanthii inoculation at different inoculation time points. These enhancements were significantly higher in Sixing F1 than Jin12 F1. Our results indicate that the Sixing F1 cultivar exhibited a much stronger ability in resistance to P. xanthii infection than the Jin12 F1 cultivar. Our results suggest that one possible mechanism of C. pepo cultivars to prevent the pathogen P. xanthii infection is by activating and enhancing the activity and gene expression of the phenylpropanoids pathway to synthesize phenolic substances and lignin, ROS scavenging defense enzymes to eliminate the harmful effects of ROS, and signaling pathway marker gene expression to improve plant disease resistance.

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Expression patterns of two pal genes of Pleurotus ostreatus across developmental stages and under heat stress

Cited by 42 publications

References 43 publications

Alternative oxidase gene induced by nitric oxide is involved in the regulation of ROS and enhances the resistance of Pleurotus ostreatus to heat stress

Alternative oxidase gene induced by nitric oxide is involved in the regulation of ROS and enhances the resistance of Pleurotus ostreatus to heat stress

Stimulating Fungi Pleurotus ostreatus with Hydrocortisone

Differential Responses of Cucurbita pepo to Podosphaera xanthii Reveal the Mechanism of Powdery Mildew Disease Resistance in Pumpkin

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