Expression profile of active genes in the human pituitary gland

Tanaka, Susumu; Tatsumi, Koichi; Okubo, Kousaku; Itoh, Koichi; Kawamoto, Shoko; Matsubara, Kazuo; Amino, Nobuyuki

doi:10.1677/jme.0.0280033

Cited by 25 publications

(21 citation statements)

References 41 publications

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“…In addition to the InhBP-L and InhBP-S isoforms described previously in humans and rats (8,20,33,38), we identified two novel InhBP/ p120 mRNA variants in mouse pituitary glands ( Fig. 2 and 3).…”

Section: Mousementioning

confidence: 53%

“…The mouse InhBP-S cDNA was amplified by RT-PCR from adult pituitary RNA. The open reading frame encodes a 232-amino-acid predicted protein that is 95 and 87% identical to the InhBP-S (or pituitary gland-specific factor-2 [PGSF2]) sequence described in rats and humans, respectively (8,38).…”

Section: Methodsmentioning

confidence: 99%

“…Two isoforms of InhBP/p120, InhBP-L and InhBP-S, have been characterized in rats and humans (8,20,33,38). InhBP-L is a large membrane-bound protein consisting of 12 C-type immunoglobulin (Ig)-like domains, a transmembrane domain, and a short cytoplasmic tail.…”

mentioning

confidence: 99%

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Normal Reproductive Function in InhBP/p120-Deficient Mice

Bernard

Burns²,

Haupt³

et al. 2003

Molecular and Cellular Biology

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The inhibins are gonadal transforming growth factor ␤ superfamily protein hormones that suppress pituitary follicle-stimulating hormone (FSH) synthesis. Recently, betaglycan and inhibin binding protein (InhBP/p120, also known as the product of immunoglobulin superfamily gene 1 [IGSF1]) were identified as candidate inhibin coreceptors, shedding light on the molecular basis of how inhibins may affect target cells. Activins, which are structurally related to the inhibins, act within the pituitary to stimulate FSH production. Betaglycan increases the affinity of inhibins for the activin type IIA (ACVR2) receptor, thereby blocking activin binding and signaling through this receptor. InhBP/p120 may not directly bind inhibins but may interact with the activin type IB receptor, ALK4, and participate in inhibin B's antagonism of activin signaling. To better understand the in vivo functions of InhBP/p120, we characterized the InhBP/p120 mRNAs and gene in mice and generated InhBP/p120 mutant mice by gene targeting in embryonic stem cells. InhBP/p120 mutant male and female mice were viable and fertile. Moreover, they showed no alterations in FSH synthesis or secretion or in ovarian or testicular function. These data contribute to a growing body of evidence indicating that InhBP/p120 does not play an essential role in inhibin biology.

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Section: Mousementioning

confidence: 53%

Section: Methodsmentioning

confidence: 99%

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Normal Reproductive Function in InhBP/p120-Deficient Mice

Bernard

Burns²,

Haupt³

et al. 2003

Molecular and Cellular Biology

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“…In addition, we identified a cluster of three intronic miRNAs (miR-25, -93, and -106b) that are likely linked to the transcription of DNA Replication Licensing Factor MCM7 (Table 2), a gene that is quiescent in nondividing cells and is upregulated just prior to S phase of the cell cycle (Fujita et al 1996). Interestingly, human miR-7-3 is contained within intron 2 of Pituitary Gland Specific Factor 1 (Table 2), a gene that is expressed in the pituitary gland (Tanaka et al 2002). This mature miRNA has two other identical copies in the genome, one of which (miR-7-1) is possibly linked to the expression of a ubiquitously expressed gene, HNRPK (Supplemental Table E).…”

Section: Genome Research 1903mentioning

confidence: 99%

Identification of Mammalian microRNA Host Genes and Transcription Units

Rodriguez¹,

Griffiths-Jones²,

Ashurst³

et al. 2004

Genome Res.

1,814

1,386

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To derive a global perspective on the transcription of microRNAs (miRNAs) in mammals, we annotated the genomic position and context of this class of noncoding RNAs (ncRNAs) in the human and mouse genomes. Of the 232 known mammalian miRNAs, we found that 161 overlap with 123 defined transcription units (TUs). We identified miRNAs within introns of 90 protein-coding genes with a broad spectrum of molecular functions, and in both introns and exons of 66 mRNA-like noncoding RNAs (mlncRNAs). In addition, novel families of miRNAs based on host gene identity were identified. The transcription patterns of all miRNA host genes were curated from a variety of sources illustrating spatial, temporal, and physiological regulation of miRNA expression. These findings strongly suggest that miRNAs are transcribed in parallel with their host transcripts, and that the two different transcription classes of miRNAs ('exonic' and 'intronic') identified here may require slightly different mechanisms of biogenesis.[Supplemental material is available online at www.genome.org.] . In Drosophila, a mutation in miR-14 leads to a disruption in normal patterns of cell death and also defects in fat metabolism (Xu et al. 2003). In mammals, ∼230 miRNAs have been identified from a vast array of tissues and cell types (Lagos-Quintana et al.

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“…Indeed, distribution of both miR-7b and FICT suggest they share regulatory elements within the promoter region [6]. In addition, the human homologue of miR-7b (miR-7-3) and its host gene PGSF1 also reveal brain-specific (including hypothalamus and pituitary) gene expression patterns [2,4,6,12].…”

Section: Introductionmentioning

confidence: 99%

miR-7b Promoter Contains Negative Gene Elements

Choi¹,

Lee²

2011

Journal of Life Science

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The typical miRNA and its nearby host gene are co-expressed by sharing the same promoter. We assumed that miR-7b and its host gene FICT might use an identical promoter for their brain specific gene expression. Sequence comparison of the genomic DNA of mouse miR-7b, human miR-7-3 and their host genes by using the bioinformatic tools revealed high sequence homology and several putative transcription factor-binding sites on the promoter region. In order to probe the hypothesis we used a luciferase vector system into which we cloned the 5' upstream conserved region of miR-7b and FICT. The putative promoter region showed decreased luciferase activity, suggesting that the 5' upstream of miR-7b and FICT contain a negative regulator for gene expression.

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Expression profile of active genes in the human pituitary gland

Cited by 25 publications

References 41 publications

Normal Reproductive Function in InhBP/p120-Deficient Mice

Normal Reproductive Function in InhBP/p120-Deficient Mice

Identification of Mammalian microRNA Host Genes and Transcription Units

miR-7b Promoter Contains Negative Gene Elements

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