Expression Profile of the Stem Cell Markers in Human Hertwig’s Epithelial Root Sheath/Epithelial Rests of Malassez Cells

Nam, Hyunwoo; Kim, Jae-Won; Park, Jaewan; Park, Joo-Cheol; Kim, Jung-Wook; Seo, Byoung‐Moo; Lee, Jae Cheoun; Lee, Gene

doi:10.1007/s10059-011-0045-3

Cited by 47 publications

(43 citation statements)

References 44 publications

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“…Tissue-specific localization of the stem cell population was reported in periodontal tissues. 37,38 The difference of mechanical stress might affect such distribution of stem cells.…”

Section: Stretch Increases Proliferation Without Differentiation In Dmentioning

confidence: 99%

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Hara

Naruse

Ozawa

et al. 2013

Tissue Engineering Part A

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Dental pulp stem cells (DPSCs), which can differentiate into several types of cells, are subjected to mechanical stress by jaw movement and occlusal forces. In this study, we evaluated how the uniaxial mechanical stretch influences proliferation and differentiation of DPSCs. DPSCs were isolated and cultured from male SpragueDawley rats. Cultured DPSCs were identified by surface markers and the differentiation capabilities as adipocytes or osteoblasts. To examine the response to mechanical stress, uniaxial stretch was exposed to cultured DPSCs. We evaluated the impact of stretch on the intracellular signaling, proliferation, osteogenic differentiation, and gene expressions of DPSCs. Stretch increased the phosphorylation of Akt, ERK1/2, and p38 MAP kinase as well as the proliferation of DPSCs. The stretch-induced proliferation of DPSCs was abolished by the inhibition of the ERK pathway. On the other hand, stretch significantly decreased the osteogenic differentiation of DPSCs, but did not affect the adipogenic differentiation. We also confirmed mRNA expressions of osteocalcin and osteopontin were significantly suppressed by stretch. In conclusion, uniaxial stretch increased the proliferation of DPSCs, while suppressing osteogenic differentiation. These results suggest a crucial role of mechanical stretch in the preservation of DPSCs in dentin. Furthermore, mechanical stretch may be a useful tool for increasing the quantity of DPSCs in vitro for regenerative medicine.

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“…Tissue-specific localization of the stem cell population was reported in periodontal tissues. 37,38 The difference of mechanical stress might affect such distribution of stem cells.…”

Section: Stretch Increases Proliferation Without Differentiation In Dmentioning

confidence: 99%

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Hara

Naruse

Ozawa

et al. 2013

Tissue Engineering Part A

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show abstract

“…They remain in periodontal tissues throughout the adult life in cord or net-like formations or as isolated islands near the cementum [14]. It is generally acknowledged that their functional role is to maintain the homeostasis of the periodontium through reciprocal interactions with other periodontal cells [15]. In recent years, there are studies suggesting that ERM cells might contain stem cell characteristics [16,17].…”

Section: Discussionmentioning

confidence: 99%

Development of supernumerary premolars in the mandible after orthodontic extraction - A case report

Guo¹,

Wang²,

Bai³

2017

Med Clin Arch

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Supernumerary premolars are relatively rare and the etiology of supernumerary tooth formation is complicated and has not yet been well known up to now. The present case documents a case of supernumerary premolars developing in the mandibular premolar region after extractions of the first premolars for orthodontic treatment in an Asian boy. What is special is that they positioned exactly where the extracted premolars used to be. Theoretical considerations for the development of them are postulated from the perspectives of tooth development and engineered tooth regeneration. Residual dental epithelial cells and mesenchyme cells in the alveolar fossas after orthodontic extraction of young permanent premolars may have the potential to proliferate, recombine and interact with each other leading to the development of supernumerary teeth under the inductive microenvironment of alveolar fossas.

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“…Also Xiong J, et al [82] has reported their capacity to carry out epithelial mesenchymal interactions. Following which numerous studies by the same authors have revealed that ECRM can differentiate into bone, cementum and periodontal ligament [80][81][82].…”

Section: Epithelial Cell Rests Of Malassez (Ecrm)mentioning

confidence: 99%

“…Since ECRM is normally present within the periodontium, hence studies have been carried out to research the stemness of ECRM [80][81][82]. They express MSC markers CD29, CD44, HSP-90β also embryonic stem cell markers Oct-4, Nanog and SSEA-4.…”

Section: Epithelial Cell Rests Of Malassez (Ecrm)mentioning

confidence: 99%

The Scope of Stem Cells in Periodontal Regeneration

George¹

2015

JDODT

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Expression Profile of the Stem Cell Markers in Human Hertwig’s Epithelial Root Sheath/Epithelial Rests of Malassez Cells

Cited by 47 publications

References 44 publications

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Development of supernumerary premolars in the mandible after orthodontic extraction - A case report

The Scope of Stem Cells in Periodontal Regeneration

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