Expression profiles and localization of vitellogenin mRNA and protein during ovarian development of the giant tiger shrimp Penaeus monodon

Hiransuchalert, Rachanimuk; Thamniemdee, Natechanok; Khamnamtong, Bavornlak; Yamano, Keisuke; Klinbunga, Sirawut

doi:10.1016/j.aquaculture.2013.07.026

Cited by 26 publications

(8 citation statements)

References 36 publications

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“…Our result suggested that PemVIH could regulate ovarian vitellogenesis in subadult females. In P. monodon , early expression of PemVg could be detected in the premature ovary of juvenile shrimp and presented throughout the ovarian maturation, especially in the vitellogenic stage ( 60 ). Moreover, a certain amount of Vg protein could also be detected in hemolymph of subadult shrimp, however, the levels were lower than in adults ( 27 , 61 ).…”

Section: Discussionmentioning

confidence: 99%

Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon

et al. 2021

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In this study, a novel Crustacean Hyperglycemic Hormone-type II gene (CHH-type II) was identified and biologically characterized in a shrimp, Penaeus monodon. Based on its structure and function, this gene was named P. monodon vitellogenesis-inhibiting hormone (PemVIH). The complete cDNA sequence of PemVIH consisted of 1,022 nt with an open reading frame (ORF) of 339 nt encoding a polypeptide of 112 amino acids. It was classified as a member of the CHH-type II family based on conserved cysteine residues, a characteristically positioned glycine residue, and the absence of CHH precursor-related peptide (CPRP) domain. The deduced mature PemVIH shared the highest sequence similarities with giant river prawn sinus gland peptide A. Unlike P. monodon gonad-inhibiting hormone (PemGIH), PemVIH was expressed only in the brain and ventral nerve cord, but not the eyestalks. Whole mount immunofluorescence using a newly generated PemVIH antiserum detected positive signals in neuronal cluster 9/11 and 17 of the brain, commissural ganglion (CoG), and neuronal clusters of ventral nerve cord. The presence of PemVIH-positive neurons in CoG, a part of stomatogastric nervous system, suggested a potential mechanism for crosstalk between nutritional and reproductive signaling. The role of PemVIH in vitellogenesis was evaluated using RNA interference technique. Temporal knockdown of PemVIH in female subadults resulted in a 3-fold increase in ovarian vitellogenin expression, suggesting an inhibitory role of PemVIH in vitellogenesis. This study provided novel insight into the control of vitellogenesis and additional strategies for improving ovarian maturation in P. monodon without the current harmful practice of eyestalk ablation.

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Section: Discussionmentioning

confidence: 99%

Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon

et al. 2021

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“…This difference suggested that eyestalk hormone regulates Vg transcription, and its target tissues for Vg inhibition are both the hepatopancreas and ovary. Although eyestalk ablation can cause a rapid increase in the mRNA of hepatopancreas and ovarian Vg during vitellogenesis and final maturation, low-quality spawns are observed in eyestalk-ablated decapod crustaceans such as P. monodon (Wongprasert et al, 2006;Hiransuchalert et al, 2013). Alternative measures should therefore be established to induce ovarian development and maturation in economically valuable crustaceans without the use of eyestalk ablation and thus obtain high-quality spawners.…”

Section: Discussionmentioning

confidence: 99%

“…Vg is synthesized exclusively in the hepatopancreas of other species such as Oziothelphusa senex (Fabricius, 1798) (Girish et al, 2014), Pandalus hypsinotus Brandt, 1851(Tsutsui et al, 2004, Macrobrachium rosenbergii (De Man, 1879) (Yang et al, 2000), and Charybdis feriata (Linnaeus, 1758) (Mak et al, 2005). Several studies have also demonstrated that Vg is synthesized in both the hepatopancreas and ovary of species such as Macrobrachium nipponense (De Man, 1879) (Bai et al, 2015), Penaeus monodon (Fabricius, 1798) (Hiransuchalert et al, 2013), Scylla paramamosain Estampador, 1950(Jia et al, 2013, Carcinus maenas Linnaeus, 1758(Ding et al, 2010, Marsupenaeus japonicus Spence Bate, 1888 (Okumura et al, 2007), and Eriocheir sinensis H. Milne Edwards, 1853 (Li et al, 2006), but the contribution of the ovary is relatively less than that of the hepatopancreas.…”

Section: Introductionmentioning

confidence: 99%

The hepatopancreas and ovary are the sites of vitellogenin synthesis in female red swamp crayfish (Procambarus clarkii (Girard, 1852)) (Decapoda: Astacoidea: Cambaridae)

Zhang

Yin

Chen

et al. 2016

Journal of Crustacean Biology

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A B S T R A C T Vitellogenin (Vg) is the precursor of vitellin, the major yolk protein. The site of Vg synthesis in crustaceans remains controversial. This study investigated the synthesis site of Vg in female red swamp crayfish Procambarus clarkii (Girard, 1852). Results revealed that the mRNA of Vg was detected in the tissues of hepatopancreas of P. clarkii. The mRNA expression level of Vg increased in the previtellogenic stage and peaked in the vitellogenic stage in the hepatopancreas and ovary; the mRNA expression level of Vg in the hepatopancreas was approximately 20-25-fold of that of the ovary in the same ovarian stage. Eyestalk ablation could upregulate the mRNA expression level of Vg in both organs. Our RNAi experiment verified that the hepatopancreas and ovary are the Vg synthesis sites in female P. clarkii.

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“…Furthermore, the hepatopancreas is an important site for the synthesis of vitellogenin. In several crustacean species, such as Carcinus maenas, Eriocheir sinensis, Marsupenaeus japonicas, Penaeus monodon, M. nipponense, and Scylla paramamosain, the hepatopancreas makes a significantly higher contribution to vitellogenin synthesis compared with the ovary [11,[16][17][18][19]. In previous studies, ovary transcriptomes of five developmental stages were constructed to detect the key pathways and genes involved in ovary maturation [6].…”

Section: Introductionmentioning

confidence: 99%

Hepatopancreas transcriptome analyses provide new insights into the molecular regulatory mechanism of fast ovary maturation in Macrobrachium nipponense

et al. 2022

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Background Macrobrachium nipponense is an economically and ecologically important freshwater prawn that is widely farmed in China. In contrast to other species of marine shrimp, M. nipponense has a short sexual maturity period, resulting in not only high stocking densities, but also a reduced survival rate and increased risk of hypoxia. Therefore, there is an urgent need to study the molecular mechanisms underlying fast ovary maturation in this species. Results Comparative transcriptome analysis was performed using hepatopancreatic tissue from female M. nipponense across five ovarian maturation stages to explore differentially expressed genes and pathways involved in ovarian maturation. In total, 118.01 Gb of data were generated from 15 transcriptomes. Approximately 90.46% of clean reads were mapped from the M. nipponense reference genome. A comprehensive comparative analysis between successive ovarian maturation stages generated 230–5814 differentially expressed genes. Gene Ontology (GO) enrichment was highly concentrated in the “biological process” category in all four comparison groups, and mainly focused on energy synthesis and accumulation, energy decomposition and transport. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment results showed that, among 20 significantly enriched KEGG pathways, nine were involved in the synthesis, degradation, and metabolism of carbohydrates, lipids, and other nutrient intermediates, suggesting that the hepatopancreas has an important role in energy supply during ovarian maturation. Furthermore, the “Insect hormone biosynthesis” pathway was found to have a dominant role in the development of the ovary from immaturity to maturity, supporting the hypothesis that ecdysteroid- and juvenile hormone-signaling pathways have an important role in hepatopancreas regulation of ovarian maturation. Conclusion Taken together, this study sheds light on the role of the hepatopancreas in the molecular regulation of ovary maturation in M. nipponense. The present study provided new insights for understanding the mechanisms of reproductive regulation in crustaceans.

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Expression profiles and localization of vitellogenin mRNA and protein during ovarian development of the giant tiger shrimp Penaeus monodon

Cited by 26 publications

References 36 publications

Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon

Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon

The hepatopancreas and ovary are the sites of vitellogenin synthesis in female red swamp crayfish (Procambarus clarkii (Girard, 1852)) (Decapoda: Astacoidea: Cambaridae)

Hepatopancreas transcriptome analyses provide new insights into the molecular regulatory mechanism of fast ovary maturation in Macrobrachium nipponense

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