Expression Profiles in Barley Microspore Embryogenesis

Abstract

In barley, microarray technology has allowed to study the gene expression profiling associated with the stress pretreatment phase of microspore embryogenesis, where the reprogramming of microspores takes place. Transcriptome analysis of anthers before and after four days of mannitol pretreatment, revealed changes in the expression of 2,673 genes.A thorough study of these differentially expressed genes indicated that microspores maintained their cell cycle machinery in a steady state during stress pretreatment and underwent a major reorganization of metabolic pathways accompanied by a multidimensional stress response. Up-regulation of transcription factors related to stress responses and changes in developmental programmes took place during the pretreatment. Preliminary studies have indicated that YABBY5, ZML2, CURLY LEAF and ICE1 transcription factors have a stress pretreatment specific induction and, therefore, could play a direct role in microspore reprogramming.

Effects of Biotin and Ascorbic Acid Applications on Haploid Embryo Induction in Semisolid and Double Layer Nutrient Media in Pepper (Capsicum annuum L.) Anther Culture

“…Microspores can be encouraged to deviate from their gametic development and transition to embryogenesis by in vitro specific stress applications (Shariatpanahi 2006, Hosp et al2007, Seguí-Simarro and Nuez 2008Munoz-Amatriain 2009, Rodriguez-Serrano et al2012, Ahmadi and Ebrahimzadeh, 2020, Perez-Perez 2019.…”

Microspore embryogenesis induction by mannitol and TSA results in a complex regulation of epigenetic dynamics and gene expression in bread wheat

“…TaYAO , TaNFD6-A , TabZIPF1 and TaAGO802 were directly related to different pollen development processes as 18S pre-rRNA processing, zinc homeostasis, DNA silencing, or other unknown functions ( Portereiko et al., 2006 ; Li et al., 2010 ; Evens et al., 2017 ; Akond et al., 2020 ). Disruption of the gametophytic developmental pathway has been reported as a key process in barley ME induction with mannitol ( Muñoz-Amatriaín et al., 2006 ; Muñoz-Amatriaín et al., 2009 ). In this study, a progressive down-regulation of most genes associated with the disruption of the gametophytic development was observed, with a tendency to be faster in Pavon.…”

“…Although different protocols are available for ME in wheat (Castillo et al, 2021;Jensen et al, 2022), their efficiency depends largely on the genotype (Lantos et al, 2013;Weigt et al, 2019;Castillo et al, 2019). The genetic control of ME is quite complex (Muñoz-Amatriaıń et al, 2008;Nielsen et al, 2015;Abd ElFath et al, 2020). Besides the genetic control, the epigenetic state of the microspores before ME induction could also play and important role.…”

Genetic Loci Governing Androgenic Capacity in Perennial Ryegrass (Lolium perenne L.)

“…The first, CURLY LEAF ( CLF ), encodes one of three SET domain proteins, the others being MEDEA ( MEA ) and SWINGER ( SWN ), which mediate large-scale chromatin remodelling during embryogenic development ( Liu et al 2016 ). In fact, the mannitol stress treatment used prior to barley IMC was found to induce the upregulation of CLF in anther tissue ( Muñoz-Amatriaín et al 2009a ). The second homolog is a FIE domain which is associated with MEA in the gametophytic- and endosperm-specific configuration of the PRC2.…”