Expression profiles of<i>Br<scp>MYB</scp></i>transcription factors related to glucosinolate biosynthesis and stress response in eight subspecies of<i>Brassica rapa</i>

Seo, Mi-Suk; Jin, Mina; Sohn, Seong-Han; Kim, Jung Sun

doi:10.1002/2211-5463.12231

Cited by 21 publications

(22 citation statements)

References 45 publications

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“…Detailed information including the chemical name and side chains is found in Refs. [ 8 , 19 , 22 , 23 , 24 ]. An abundance of GRH was well demonstrated in previous studies on radish roots [ 17 , 18 ].…”

Section: Resultsmentioning

confidence: 99%

Induction of Glucoraphasatin Biosynthesis Genes by MYB29 in Radish (Raphanus sativus L.) Roots

Kang

Won

Seo

et al. 2020

IJMS

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Glucoraphasatin (GRH) is a specific aliphatic glucosinolate (GSL) that is only abundant in radish (Raphanus sativus L.). The gene expression regulating GRH biosynthesis in radish is still poorly understood. We employed a total of 59 radish accessions to analyze GSL profiles and showed that GRH was specific and predominant among the aliphatic GSLs in radish roots. We selected five accessions roots with high, moderate and low GSL biosynthesis, respectively, to conduct a comparative transcriptome analysis and the qRT-PCR of the biosynthesis genes for aliphatic GSLs. In this study, among all the accessions tested, roots with the accession RA157-74 had a high GRH content and showed a significant expression of the aliphatic GSL biosynthesis genes. We defined the genes involved in the GRH biosynthesis process and found that they were regulated by a transcription factor (RSG00789) at the MYB29 locus in radish roots. We found 13 aliphatic GSL biosynthesis genes regulated by the RSG00789 gene in the GRH biosynthesis pathway.

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Section: Resultsmentioning

confidence: 99%

Induction of Glucoraphasatin Biosynthesis Genes by MYB29 in Radish (Raphanus sativus L.) Roots

Kang

Won

Seo

et al. 2020

IJMS

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“…Finally, the side chains of the core structures are modified by oxidation, elimination, alkylation or esterification in the aliphatic and indolic groups [ 14 ]. Aliphatic GSLs are the most abundant, at about 57–97% of the total GSL content, while aromatic GSLs are minor components in Brassica species [ 24 , 25 , 26 ]. The GSLs of the aliphatic and aromatic groups have unique properties for human health and livestock and have roles in oncogenesis, disease, and nutrition [ 27 ].…”

Section: Metabolic and Regulatory Pathways Of Gsl Biosynthesismentioning

confidence: 99%

“…Consequently, variations in gene length (in base pair) and nonsynonymous amino acid sequences are caused by polymorphisms in the downstream C-terminal region. We have identified and reported the conserved R2R3 MYB DNA-binding domains of 13 MYB TFs related to GSL biosynthesis in B. rapa [ 16 , 24 ]. A phylogenetic tree was constructed using the amino acid sequences of 61 R2R3 MYB DNA-binding domains containing A. thaliana to elucidate evolutionary relationships ( Figure 3 ).…”

Section: Myb Tfs Involved In Gsl Biosynthesis In Genome-sequenced mentioning

confidence: 99%

“…Many putative biosynthetic and regulatory genes related to GSL biosynthesis have been identified in the genome sequence of B. rapa [ 7 ]. The 13 BrMYB TFs that possess a complete coding sequence indicate that paralogous genes arising through gene duplication have led to functional diversity and changes in expression patterns, reflected by genotype-specific variation in B. rapa subspecies [ 24 ]. The expression of some BrMYB TFs, such as BrMYB28 , BrMYB34 , and BrMYB51 also increased under abiotic and biotic stress conditions.…”

Section: Myb Tfs Involved In Gsl Biosynthesis In Genome-sequenced mentioning

confidence: 99%

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Understanding of MYB Transcription Factors Involved in Glucosinolate Biosynthesis in Brassicaceae

Seo

Kim

2017

Molecules

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Glucosinolates (GSLs) are widely known secondary metabolites that have anticarcinogenic and antioxidative activities in humans and defense roles in plants of the Brassicaceae family. Some R2R3-type MYB (myeloblastosis) transcription factors (TFs) control GSL biosynthesis in Arabidopsis. However, studies on the MYB TFs involved in GSL biosynthesis in Brassica species are limited because of the complexity of the genome, which includes an increased number of paralog genes as a result of genome duplication. The recent completion of the genome sequencing of the Brassica species permits the identification of MYB TFs involved in GSL biosynthesis by comparative genome analysis with A. thaliana. In this review, we describe various findings on the regulation of GSL biosynthesis in Brassicaceae. Furthermore, we identify 63 orthologous copies corresponding to five MYB TFs from Arabidopsis, except MYB76 in Brassica species. Fifty-five MYB TFs from the Brassica species possess a conserved amino acid sequence in their R2R3 MYB DNA-binding domain, and share close evolutionary relationships. Our analysis will provide useful information on the 55 MYB TFs involved in the regulation of GSL biosynthesis in Brassica species, which have a polyploid genome.

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“…Recently, Seo et al (2016) found that in Brassica rapa ( Br ) genome, BrMYB28 genes were identified as three orthologous copies to Arabidopsis thaliana ( AtMYB28 ). Particularly overexpression of three BrMYB28 genes such as BrMYB28.1 , BrMYB28.2 and BrMYB28.3 are responsible for the higher synthesis of GSL [ 9 ]. Subsequently, several activated products of GSLs are beneficial to human and animal health [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Carcinogenic Glucosinolates in Cruciferous Vegetables and Their Antagonistic Effects on Prevention of Cancers

2018

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Glucosinolates (GSL) are naturally occurring β-d-thioglucosides found across the cruciferous vegetables. Core structure formation and side-chain modifications lead to the synthesis of more than 200 types of GSLs in Brassicaceae. Isothiocyanates (ITCs) are chemoprotectives produced as the hydrolyzed product of GSLs by enzyme myrosinase. Benzyl isothiocyanate (BITC), phenethyl isothiocyanate (PEITC) and sulforaphane ([1-isothioyanato-4-(methyl-sulfinyl) butane], SFN) are potential ITCs with efficient therapeutic properties. Beneficial role of BITC, PEITC and SFN was widely studied against various cancers such as breast, brain, blood, bone, colon, gastric, liver, lung, oral, pancreatic, prostate and so forth. Nuclear factor-erythroid 2-related factor-2 (Nrf2) is a key transcription factor limits the tumor progression. Induction of ARE (antioxidant responsive element) and ROS (reactive oxygen species) mediated pathway by Nrf2 controls the activity of nuclear factor-kappaB (NF-κB). NF-κB has a double edged role in the immune system. NF-κB induced during inflammatory is essential for an acute immune process. Meanwhile, hyper activation of NF-κB transcription factors was witnessed in the tumor cells. Antagonistic activity of BITC, PEITC and SFN against cancer was related with the direct/indirect interaction with Nrf2 and NF-κB protein. All three ITCs able to disrupts Nrf2-Keap1 complex and translocate Nrf2 into the nucleus. BITC have the affinity to inhibit the NF-κB than SFN due to the presence of additional benzyl structure. This review will give the overview on chemo preventive of ITCs against several types of cancer cell lines. We have also discussed the molecular interaction(s) of the antagonistic effect of BITC, PEITC and SFN with Nrf2 and NF-κB to prevent cancer.

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Expression profiles ofBrMYBtranscription factors related to glucosinolate biosynthesis and stress response in eight subspecies ofBrassica rapa

Cited by 21 publications

References 45 publications

Induction of Glucoraphasatin Biosynthesis Genes by MYB29 in Radish (Raphanus sativus L.) Roots

Induction of Glucoraphasatin Biosynthesis Genes by MYB29 in Radish (Raphanus sativus L.) Roots

Understanding of MYB Transcription Factors Involved in Glucosinolate Biosynthesis in Brassicaceae

Anti-Carcinogenic Glucosinolates in Cruciferous Vegetables and Their Antagonistic Effects on Prevention of Cancers

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