Expression profiling of c-kit and its impact after esiRNA silencing during gonadal development in catfish

Laldinsangi, C.; Senthilkumaran, B.

doi:10.1016/j.ygcen.2018.04.004

Cited by 6 publications

(3 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, SYCP3 was upregulated in testis. In agreement with our result, this gene was examined to reflect the level of meiosis and spermatogenesis in zebrafish, tilapia and catfish [33][34][35]. In addition, BMP15 belongs to the subgroup of transforming growth factor β (TGFβ) superfamily, and plays important roles in regulating ovarian functions [36].…”

Section: Plos Onesupporting

confidence: 66%

De novo transcriptome assembly of four organs of Collichthys lucidus and identification of genes involved in sex determination and reproduction

et al. 2020

View full text Add to dashboard Cite

The spinyhead croaker (Collichthys lucidus) is a commercially important fish species, which is mainly distributed in the coastal regions of China. However, little is known about the molecular regulatory mechanism underlying reproduction in C. lucidus. A de novo transcriptome assembly in brain, liver, ovary and testis tissues of C. lucidus was performed. Illumina sequencing generated 60,322,004, 57,044,284, 60,867,978 and 57,087,688 clean reads from brain, liver, ovary and testis tissues of C. lucidus, respectively. Totally, 131,168 unigenes with an average length of 644 bp and an N50 value of 1033 bp were assembled. In addition, 1288 genes were differentially expressed between ovary and testis, including 442 up-regulated and 846 down-regulated in ovary. Functional analysis revealed that the differentially expressed genes between ovary and testis were mainly involved in the function of sexual reproduction, sex differentiation, development of primary male sexual characteristics, female gamete generation, and male sex differentiation. A number of genes which might be involved in the regulation of reproduction and sex determination were found, including HYAL and SYCP3 and BMP15. Furthermore, 35,476 simple sequence repeats (SSRs) were identified in this transcriptome dataset, which would contribute to further genetic and mechanism researches. De novo transcriptome sequencing analysis of four organs of C. lucidus provides rich resources for understanding the mechanism of reproductive development of C. lucidus and further investigation of the molecular regulation of sex determination and reproduction of C. lucidus.

show abstract

Section: Plos Onesupporting

confidence: 66%

De novo transcriptome assembly of four organs of Collichthys lucidus and identification of genes involved in sex determination and reproduction

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Several genes involved in sex determination/differentiation, such as dmrt1 , sox9 , foxl2 , bcar1 [ 135 ], and cyp19 in catfish have been also identified. In addition to this, another study in catfish revealed the role of ckit in germ cell proliferation, development, and maturation during gonadal recrudescence [ 136 ]. Dimorphic expression of various transcription factors and steroidogenic enzyme genes has been demonstrated by Raghuveer et al [ 47 ] during critical period of gonadal differentiation in catfish.…”

Section: Gonad Genesismentioning

confidence: 99%

“…In many animal models including catfish, RNA knockdown can be achieved more feasibly using siRNA, shRNA or esiRNA. In this line, in vivo and in vitro transient gene silencing using PEI mediated siRNA/shRNA/esiRNA has been standardized and well established at tissue and cellular levels in gonads and brain as well as at animal level in our laboratory using various fish models including catfish [ 67 , 85 , 136 , 165 , 188 , 220 , 221 ] to functionally characterize many important factors related to teleostean reproduction. In addition, Senthilkumaran [ 168 ] compared mammalian and piscine oocyte maturation with a note on sperm maturation citing the involvement of hsd20b vis-à-vis 17α,20β-DP in addition to T and 11-KT [ 140 , 222 ].…”

Section: Gene Knockout/knockdown/sirna Based Transient Gene Silencingmentioning

confidence: 99%

Advances in Reproductive Endocrinology and Neuroendocrine Research Using Catfish Models

Senthilkumaran

Kar

2021

Cells

View full text Add to dashboard Cite

Catfishes, belonging to the order siluriformes, represent one of the largest groups of freshwater fishes with more than 4000 species and almost 12% teleostean population. Due to their worldwide distribution and diversity, catfishes are interesting models for ecologists and evolutionary biologists. Incidentally, catfish emerged as an excellent animal model for aquaculture research because of economic importance, availability, disease resistance, adaptability to artificial spawning, handling, culture, high fecundity, hatchability, hypoxia tolerance and their ability to acclimate to laboratory conditions. Reproductive system in catfish is orchestrated by complex network of nervous, endocrine system and environmental factors during gonadal growth as well as recrudescence. Lot of new information on the molecular mechanism of gonadal development have been obtained over several decades which are evident from significant number of scientific publications pertaining to reproductive biology and neuroendocrine research in catfish. This review aims to synthesize key findings and compile highly relevant aspects on how catfish can offer insight into fundamental mechanisms of all the areas of reproduction and its neuroendocrine regulation, from gametogenesis to spawning including seasonal reproductive cycle. In addition, the state-of-knowledge surrounding gonadal development and neuroendocrine control of gonadal sex differentiation in catfish are comprehensively summarized in comparison with other fish models.

show abstract

Ovarian nesfatin-1 in Hemidactylus flaviviridis: Reproductive phase-dependent expression, role and hormonal regulation

Dotania,

Tripathy,

Rai

2024

Comparative Biochemistry and Physiology Part A: Molecular &

View full text Add to dashboard Cite

Expression profiling of c-kit and its impact after esiRNA silencing during gonadal development in catfish

Cited by 6 publications

References 69 publications

De novo transcriptome assembly of four organs of Collichthys lucidus and identification of genes involved in sex determination and reproduction

De novo transcriptome assembly of four organs of Collichthys lucidus and identification of genes involved in sex determination and reproduction

Advances in Reproductive Endocrinology and Neuroendocrine Research Using Catfish Models

Ovarian nesfatin-1 in Hemidactylus flaviviridis: Reproductive phase-dependent expression, role and hormonal regulation

Contact Info

Product

Resources

About