Expression Profiling of Circular RNAs and Micrornas in Heart Tissue of Mice with Alcoholic Cardiomyopathy

Yang, Yuqiao; Chen, Hongmei; Ding, Nina; Wang, Shuo; Duan, Zhantao; Birnbaum, Yochai; Ye, Yumei; Qian, Jinqiao

doi:10.1159/000489596

Cited by 12 publications

(8 citation statements)

References 44 publications

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“…Therefore, many investigators have demonstrated that circRNAs play important roles in the pathogenesis of a number of brainrelated disorders such as multiple system atrophy [74], transient focal ischemia and stroke [75][76][77][78][79][80][81][82], neuropsychiatric disorders [64,68], Alzheimer's disease [83,84], Parkinson's disease [85], brain bacterial infection [86], brain tumors [87], and metabolic diseases [88]. Second, circRNAs are also highly expressed in the heart [89] and are reported to play a role in cardiac remodeling [90], stress response [91], endothelial-to-mesenchymal transition [92], metabolism [93,94], immune tolerance [95], atrial fibrillation [96], and apoptosis [97]. Thus, these circRNAs are suggested to participate in heart diseases such as heart failure [91,97,98], ventricular septal defect [99], chronic heart disease [92,[100][101][102], alcoholic cardiomyopathy [93], and rheumatic heart disease [96].…”

Section: Major Biological Function and Disease Relevancementioning

confidence: 99%

“…Second, circRNAs are also highly expressed in the heart [89] and are reported to play a role in cardiac remodeling [90], stress response [91], endothelial-to-mesenchymal transition [92], metabolism [93,94], immune tolerance [95], atrial fibrillation [96], and apoptosis [97]. Thus, these circRNAs are suggested to participate in heart diseases such as heart failure [91,97,98], ventricular septal defect [99], chronic heart disease [92,[100][101][102], alcoholic cardiomyopathy [93], and rheumatic heart disease [96]. In the lung, circRNAs have been found to be important in lung fibrosis [103], cell growth [104], cell migration and invasion [105][106][107][108], cancer tumorigenesis [109], and so on; thus, these circRNAs play a role in various lung-related diseases such as lung cancer [105,107,[110][111][112], lung injury [113], and pulmonary hypertension [114].…”

Section: Major Biological Function and Disease Relevancementioning

confidence: 99%

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Disease-Associated Circular RNAs: From Biology to Computational Identification

Tang

Kui

Lu³

et al. 2020

BioMed Research International

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Circular RNAs (circRNAs) are endogenous RNAs with a covalently closed continuous loop, generated through various backsplicing events of pre-mRNA. An accumulating number of studies have shown that circRNAs are potential biomarkers for major human diseases such as cancer and Alzheimer’s disease. Thus, identification and prediction of human disease-associated circRNAs are of significant importance. To this end, a computational analysis-assisted strategy is indispensable to detect, verify, and quantify circRNAs for downstream applications. In this review, we briefly introduce the biology of circRNAs, including the biogenesis, characteristics, and biological functions. In addition, we outline about 30 recent bioinformatic analysis tools that are publicly available for circRNA study. Principles for applying these computational strategies and considerations will be briefly discussed. Lastly, we give a complete survey on more than 20 key computational databases that are frequently used. To our knowledge, this is the most complete and updated summary on publicly available circRNA resources. In conclusion, this review summarizes key aspects of circRNA biology and outlines key computational strategies that will facilitate the genome-wide identification and prediction of circRNAs.

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Section: Major Biological Function and Disease Relevancementioning

confidence: 99%

Section: Major Biological Function and Disease Relevancementioning

confidence: 99%

Disease-Associated Circular RNAs: From Biology to Computational Identification

Tang

Kui

Lu³

et al. 2020

BioMed Research International

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“…Interestingly, microRNAs emerge as attractive biomarkers of cancer as microRNA profiling seems to more accurately cluster different types of cancer rather than mRNA ( Lu et al, 2005 ). ( Gilad et al, 2008 ; Yang et al, 2018 ). It is tempting to consider microRNAs as an attractive alternative to traditional markers of alcohol consumption in AUD ( Rosato et al, 2019 ; Lim et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Non-Invasive microRNA Profiling in Saliva can Serve as a Biomarker of Alcohol Exposure and Its Effects in Humans

Mead

Boulghassoul-Pietrzykowska

Wang

et al. 2022

Front. Genet.

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Alcohol Use Disorder (AUD) is one of the most prevalent mental disorders worldwide. Considering the widespread occurrence of AUD, a reliable, cheap, non-invasive biomarker of alcohol consumption is desired by healthcare providers, clinicians, researchers, public health and criminal justice officials. microRNAs could serve as such biomarkers. They are easily detectable in saliva, which can be sampled from individuals in a non-invasive manner. Moreover, microRNAs expression is dynamically regulated by environmental factors, including alcohol. Since excessive alcohol consumption is a hallmark of alcohol abuse, we have profiled microRNA expression in the saliva of chronic, heavy alcohol abusers using microRNA microarrays. We observed significant changes in salivary microRNA expression caused by excessive alcohol consumption. These changes fell into three categories: downregulated microRNAs, upregulated microRNAs, and microRNAs upregulated de novo. Analysis of these combinatorial changes in microRNA expression suggests dysregulation of specific biological pathways leading to impairment of the immune system and development of several types of epithelial cancer. Moreover, some of the altered microRNAs are also modulators of inflammation, suggesting their contribution to pro-inflammatory mechanisms of alcohol actions. Establishment of the cellular source of microRNAs in saliva corroborated these results. We determined that most of the microRNAs in saliva come from two types of cells: leukocytes involved in immune responses and inflammation, and buccal cells, involved in development of epithelial, oral cancers. In summary, we propose that microRNA profiling in saliva can be a useful, non-invasive biomarker allowing the monitoring of alcohol abuse, as well as alcohol-related inflammation and early detection of cancer.

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“…Twenty‐four 8‐week‐old male C57BL/6 mice (Beijing Vital River Laboratory Animal Technology Co., Ltd., Beijing, China) were randomly divided into 2 ethanol (EtOH)‐fed groups and 2 control‐fed groups (non‐EtOH fed) ( n = 6 for all groups). In EtOH‐fed groups, mice were fed a 4%‐EtOH (w/v, 28% of total calories) alcoholic liquid diet of Lieber–DeCarli or AIN‐93 for chronic alcohol exposure for 180 days (Bertola et al., ; Kryzhanovskii et al., ; Matyas et al., ; Yang et al., ). In control‐fed groups, mice were fed with non‐EtOH liquid diets, with an equal number of calories as the EtOH‐fed groups.…”

Section: Methodsmentioning

confidence: 99%

AIN‐93 Diet as an Alternative Model to Lieber–DeCarli Diet for Alcoholic Cardiomyopathy

Cao

Zhang

et al. 2019

Alcoholism Clin & Exp Res

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Background: The Lieber-DeCarli alcoholic liquid diet is a classical method for establishing animal models of alcoholic cardiomyopathy (ACM). No study has reported whether the AIN-93 diet, which is widely used as a standard diet for both long-term and short-term studies with laboratory animals, could be used to construct the ACM animal model. The present study intended to investigate whether the AIN-93 diet could be used to establish a mouse ACM model.Methods: Twenty-four C57BL/6 male mice were randomly divided into 4 equally sized groups. In ethanol (EtOH)-fed groups, mice were fed a 4%-EtOH (w/v, 28% of total calories) alcoholic liquid diet of Lieber-DeCarli or the AIN-93 diet for chronic alcohol exposure for 180 days. In control-fed groups, mice were fed with non-EtOH liquid diets with the same calories as EtOH-fed groups. Morphological observations of the hearts and molecular investigation of the brain natriuretic peptide (BNP) were carried out by echocardiography, hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining, real-time quantitative polymerase chain reaction (qPCR), and enzyme-linked immunosorbent assay.Results: Echocardiography showed that mice fed with either the 4%-EtOH Lieber-DeCarli diet or the 4%-EtOH AIN-93 diet had dilated ventricles and poor cardiac function. IHC staining of BNP, qPCR of BNP mRNA, and plasma concentration of BNP showed an up-regulated expression in mice fed with both the 4%-EtOH Lieber-DeCarli and 4%-EtOH AIN-93 diets. Less fatty liver was also observed in mice fed the AIN-93 alcoholic diet than those fed the Lieber-DeCarli alcoholic diet.Conclusions: The AIN-93 alcoholic liquid diet can be used to establish ACM animal models, as with the conventional Lieber-DeCarli alcoholic liquid diet.

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Expression Profiling of Circular RNAs and Micrornas in Heart Tissue of Mice with Alcoholic Cardiomyopathy

Cited by 12 publications

References 44 publications

Disease-Associated Circular RNAs: From Biology to Computational Identification

Disease-Associated Circular RNAs: From Biology to Computational Identification

Non-Invasive microRNA Profiling in Saliva can Serve as a Biomarker of Alcohol Exposure and Its Effects in Humans

AIN‐93 Diet as an Alternative Model to Lieber–DeCarli Diet for Alcoholic Cardiomyopathy

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