Expression Regulation and Physiological Role of Transcription Factor FOXO3a During Ovarian Follicular Development

Zhang, Hong; Lin, Fengping; Zhao, Jiu-Hua; Wang, Zhengchao

doi:10.3389/fphys.2020.595086

Cited by 23 publications

(14 citation statements)

References 68 publications

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“…It is currently established that Foxo3a can enhance transcriptional regulation of its target genes, thereby inducing oocyte apoptosis, and inhibiting the activation of primordial follicles. Namely, Wnt signaling reduces the number of primordial follicles transformed into mature follicles, thus preserving the follicular reserves ( 52 ). In terms of individual genes, we found that several piRNAs tend to be negatively correlated with genes regulating granulosa cell proliferation and follicle development.…”

Section: Discussionmentioning

confidence: 99%

Expression characteristics of piRNAs in ovine luteal phase and follicular phase ovaries

Zhang

Ren

et al. 2022

Front. Vet. Sci.

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PIWI-interacting RNAs (piRNAs), as a novel class of small non-coding RNAs that have been shown to be indispensable in germline integrity and stem cell development. However, the expressed characteristics and regulatory roles of piRNAs during different reproductive phases of animals remain unknown. In this study, we investigated the piRNAs expression profiles in ovaries of sheep during the luteal phase (LP) and follicular phase (FP) using the Solexa sequencing technique. A total of 85,219 and 1,27,156 piRNAs tags were identified in ovine ovaries across the two phases. Most expressed piRNAs start with uracil. piRNAs with a length of 24 nt or 27–29 nts accounted for the largest proportion. The obvious ping-pong signature appeared in the FP ovary. The piRNA clusters in the sheep ovary were unevenly distributed on the chromosomes, with high density on Chr 3 and 1. For genome distribution, piRNAs in sheep ovary were mainly derived from intron, CDS, and repeat sequence regions. Compared to the LP ovary, a greater number of expressed piRNA clusters were detected in the FP ovary. Simultaneously, we identified 271 differentially expressed (DE) piRNAs between LP and FP ovaries, with 96 piRNAs upregulated and 175 piRNAs downregulated, respectively. Functional enrichment analysis (GO and KEGG) indicated that their target genes were enriched in reproduction-related pathways including oocyte meiosis, PI3K-Akt, Wnt, and TGF-β signaling pathways. Together, our results highlighted the sequence and expression characteristics of the piRNAs in the sheep ovary, which will help us understand the roles of piRNAs in the ovine estrus cycle.

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Section: Discussionmentioning

confidence: 99%

Expression characteristics of piRNAs in ovine luteal phase and follicular phase ovaries

Zhang

Ren

et al. 2022

Front. Vet. Sci.

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“…For example, GRX2 exerts cardioprotective effects by activating the Nrf2 pathway via upregulation of Akt and GSK-3 β phosphorylation to abate cardiomyocyte apoptosis, oxidative stress, and inflammation in H/R injury [ 26 ]. Akt can regulate the activity of FOXO3a, one of the main downstream effectors of Akt [ 27 ]. H 2 S dampens DOX-induced apoptosis and oxidative stress in cardiomyocytes by heightening phosphorylation of Akt and FOXO3a, thereby attenuating cardiotoxic effects [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

α-Cyperone Protects Cardiomyocytes against Oxygen-Glucose Deprivation-Induced Inflammation and Oxidative Stress by Akt/FOXO3a/NF-κB Pathway

Yao

Zhu

2022

Disease Markers

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Objective. This study is aimed at investigating the mechanism of α-cyperone in oxygen and glucose deprivation- (OGD-) induced myocardial injury. Methods. Cardiomyocytes were exposed to OGD and then treated with α-cyperone. The cell counting kit-8 (CCK-8) assay and flow cytometry were performed to determine cell proliferation and apoptosis, respectively. The expression of inflammatory factors was monitored by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The profiles of apoptosis-related proteins, inflammatory proteins, and the Akt/FOXO3a/NF-κB pathway were determined by western blot. The phosphorylation of Akt, FOXO3a, and NF-κB was determined by immunofluorescence assay. The superoxide dismutase (SOD) activity and the malondialdehyde (MDA) content were gauged by the colorimetric method, and the reactive oxygen species (ROS) content was measured. Results. α-Cyperone hindered OGD-induced inflammation, oxidative stress, and apoptosis in cardiomyocytes. OGD activated the FOXO3a/NF-κB pathway and hampered the Akt phosphorylation. α-cyperone reversed OGD-mediated FOXO3a/NF-κB pathway activation. Treatment with MK-2206 abated the protective effect of α-cyperone against OGD-induced myocardial injury. The addition of α-cyperone to cardiomyocytes following Bay11-7082 treatment had no conspicuous effect on the viability and apoptosis. Conclusions. α-Cyperone protected cardiomyocytes against OGD-induced inflammation and oxidative stress via the Akt/FOXO3a/NF-κB axis.

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“…FoxO3a plays a role in the regulation of trophoblast development and pregnancy complications, and a decreased expression of FoxO3a was found in the placenta of PE cases and was present in trophoblasts ( Chen et al, 2021 ). Furthermore, FoxO3a posttranslational modifications were increased by accumulated reactive oxygen species (ROS) under oxidative stress ( Zhang H. et al, 2020 ). Therefore, these results could be an indirect result of this cellular stress induced by L-NAME and more easily controlled with anti-free radical agents such as vitamins C and D. Here, our results showed that the FoxO3a protein level was downregulated in trophoblasts under NOS inhibition.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of Fibrinogen-Like 1 Expression Contributes to Reducing the Progression of Preeclampsia

Cheng

et al. 2021

Front. Cell Dev. Biol.

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Fibrinogen-like 1 (FGL1) is involved in liver injury and liver regeneration, but its role in placenta and preeclampsia (PE) remains unclear. We assessed FGL1 expression in serum and placenta from L-NAME-induced PE-like mouse and in women with (n = 38) and without (n = 42) PE. For the mouse study, pregnant C57Bl/6 mouse (n = 6/group) were subcutaneously administered L-NAME with or without FGL1 once daily starting on days 7–14 of pregnancy and were sacrificed on gestational day (GD) 20. Maternal body weight, blood pressure, and urinary protein were assessed during GDs 8–20. The weight and length of the placenta and fetus were assessed. The placental structure was evaluated using hematoxylin staining. In the human study, the sera of the pregnant women during the late trimester were assessed with enzyme-linked immunosorbent assays (ELISAs). FGL1 expression in human trophoblast cell lines under L-NAME stimulation was measured using Western blotting and immunofluorescence staining. The detected FGL1 protein levels in serum and placenta were both significantly upregulated in patients and mouse with PE compared with those in the non-PE groups. FGL1 treatment decreased maternal hypertension and proteinuria, decreased fetal weight in mouse with PE, downregulated proinflammatory cytokine (interleukin-1b and interleukin-6) levels, and maintained the balance between antiangiogenic (fms-like tyrosine kinase-1) and proangiogenic (placental growth factor) substances in the placenta. L-NAME-upregulated FGL1 expression was inhibited following overexpression of FoxO3a. In summary, FoxO3a reduction is a potential pathophysiological mechanism leading to upregulated placental FGL1 expression that may play a pivotal role in preventing PE progression.

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Expression Regulation and Physiological Role of Transcription Factor FOXO3a During Ovarian Follicular Development

Cited by 23 publications

References 68 publications

Expression characteristics of piRNAs in ovine luteal phase and follicular phase ovaries

Expression characteristics of piRNAs in ovine luteal phase and follicular phase ovaries

α-Cyperone Protects Cardiomyocytes against Oxygen-Glucose Deprivation-Induced Inflammation and Oxidative Stress by Akt/FOXO3a/NF-κB Pathway

Upregulation of Fibrinogen-Like 1 Expression Contributes to Reducing the Progression of Preeclampsia

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