Expression study of soybean germin-like gene family reveals a role of<i>GLP7</i>gene in various abiotic stress tolerances

Li, Yongguang; Zhang, Dayong; Li, Weina; Mallano, Ali Inayat; Zhang, Yuhang; Wang, Tao; Lu, Mo; Qin, Zhiwei; Li, Wenbin

doi:10.1139/cjps-2015-0213

Cited by 15 publications

(10 citation statements)

References 24 publications

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“…One of the main roles of the proteins mentioned above is triggering the abiotic stress-tolerance in many plant species. Li et al (2016) revealed that GLP transcripts were plentiful after treatment with high salinity, PEG6000, abscisic acid, and methyl viologen in soybean. Arabidopsis plants overexpressing a GLP from soybean indicated enhanced drought, salt, and oxidative tolerance [58].…”

Section: Functional Analysis Of Salt-regulated Genes Using Mapmanmentioning

confidence: 99%

Transcriptome analysis of bread wheat leaves in response to salt stress

et al. 2021

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Salinity is one of the main abiotic stresses limiting crop productivity. In the current study, the transcriptome of wheat leaves in an Iranian salt-tolerant cultivar (Arg) was investigated in response to salinity stress to identify salinity stress-responsive genes and mechanisms. More than 114 million reads were generated from leaf tissues by the Illumina HiSeq 2500 platform. An amount of 81.9% to 85.7% of reads could be mapped to the wheat reference genome for different samples. The data analysis led to the identification of 98819 genes, including 26700 novel transcripts. A total of 4290 differentially expressed genes (DEGs) were recognized, comprising 2346 up-regulated genes and 1944 down-regulated genes. Clustering of the DEGs utilizing Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that transcripts associated with phenylpropanoid biosynthesis, transporters, transcription factors, hormone signal transduction, glycosyltransferases, exosome, and MAPK signaling might be involved in salt tolerance. The expression patterns of nine DEGs were investigated by quantitative real-time PCR in Arg and Moghan3 as the salt-tolerant and susceptible cultivars, respectively. The obtained results were consistent with changes in transcript abundance found by RNA-sequencing in the tolerant cultivar. The results presented here could be utilized for salt tolerance enhancement in wheat through genetic engineering or molecular breeding.

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Section: Functional Analysis Of Salt-regulated Genes Using Mapmanmentioning

confidence: 99%

Transcriptome analysis of bread wheat leaves in response to salt stress

et al. 2021

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“…Previous studies have suggested that extracellular germins and germin-like proteins (GLPs) could be induced by a range of abiotic or biotic stresses, such as herbivores 7 , 8 , drought 9 , 10 , salinity 8 , 11 and they are considered to be the pathogenesis-related proteins (PRs) 16 family due to their disease resistance property 12 – 14 . Germins and GLPs were first characterized in wheat ( Triticum aestivum ) and constitute a large plant gene family 15 .…”

Section: Introductionmentioning

confidence: 99%

Molecular evidence for the involvement of cotton GhGLP2, in enhanced resistance to Verticillium and Fusarium Wilts and oxidative stress

Pei

Zhu

Jia

et al. 2020

Sci Rep

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Germin-like proteins (GLps) are a diverse and ubiquitous family of plant glycoproteins belonging to the cupin super family; they play considerable roles in plant responses against various abiotic and biotic stresses. Here, we provide evidence that GLP2 protein from cotton (Gossypium hirsutum) functions in plant defense responses against Verticillium dahliae, Fusarium oxysporum and oxidative stress. Purified recombinant GhGLP2 exhibits superoxide dismutase (SOD) activity and inhibits spore germination of pathogens. Virus-induced silencing of GhGLP2 in cotton results in increased susceptibility to pathogens, plants exhibited severe wilt on leaves, enhanced vascular browning and suppressed callose deposition. transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing GhGLP2 showed significant resistance to V. dahliae and F. oxysporum, with reduced mycelia growth, increased callose deposition and cell wall lignification at infection sites on leaves. The enhanced tolerance of GhGLP2-transgenic Arabidopsis to oxidative stress was investigated by methyl viologen and ammonium persulfate treatments, along with increased H 2 o 2 production. further, the expression of several defense-related genes (PDF1.2, LOX2, and VSP1) or oxidative stress-related genes (RbohD, RbohF) was triggered by GhGLP2. Thus, our results confirmed the involvement of GhGLP2 in plant defense response against Verticillium and Fusarium wilt pathogens and stress conditions. Cotton (Gossypium hirsutum L.) is an important fiber crop that is considered the backbone of the global fiber economy 1. Verticillium and Fusarium wilt are caused by Verticillium dahliae and Fusarium oxysporum, respectively, which are soil-borne pathogenic fungi that present major constraints to the production of cotton 2. Verticillium wilt is a notorious and devastating disease of cotton 3 , and it occurs before the squaring stage and peaks in the boll-setting stage, causing necrotic areas on the leaves, wilting, and discoloration of the vascular tissues 4. The disease symptoms of Fusarium wilt initiate and peak at the seedling and squaring stages, respectively, showing necrotic patches between the main veins and leaf detachment from the stem 5. Due to diverse factors, such as the climate, pathogen population structures, and cultivar susceptibility, the currently available control measures for these two diseases are not adequate 6. Thus, research on the cultivation of resistant cotton plants, by finding novel disease-resistant genes against these soil-borne fungal species, is consequently, of great importance. Previous studies have suggested that extracellular germins and germin-like proteins (GLPs) could be induced by a range of abiotic or biotic stresses, such as herbivores 7,8 , drought 9,10 , salinity 8,11 and they are considered to be the pathogenesis-related proteins (PRs) 16 family due to their disease resistance property 12-14. Germins and GLPs were first characterized in wheat (Triticum aestivum) and constitute a large plant gene family 15. They occur as water-solub...

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“…All peanut GLPs changed their expression levels (were mostly transiently induced) in response to exogenous applications of ABA in seedlings (Wang et al 2013 ). In soybean, GLPs from leaves accumulated in response to ABA and PEG (Li et al 2016 ). The expression of the rice germin-like protein OsGLP2-1 is increased in response to ABA and has been shown to be involved in the regulation of seed dormancy (Wang et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

The dehydration- and ABA-inducible germin-like protein CpGLP1 from Craterostigma plantagineum has SOD activity and may contribute to cell wall integrity during desiccation

Giarola

Chen

Dulitz

et al. 2020

Planta

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Main conclusion CpGLP1 belongs to the large group of germin-like proteins and comprises a cell wall-localized protein which has superoxide dismutase activity and may contribute towards ROS metabolism and cell wall folding during desiccation. Abstract The plant cell wall is a dynamic matrix and its plasticity is essential for cell growth and processing of environmental signals to cope with stresses. A few so-called resurrection plants like Craterostigma plantagineum survive desiccation by implementing protection mechanisms. In C. plantagineum, the cell wall shrinks and folds upon desiccation to avoid mechanical and oxidative damage which contributes to cell integrity. Despite the high toxic potential, ROS are important molecules for cell wall remodeling processes as they participate in enzymatic reactions and act as signaling molecules. Here we analyzed the C. plantagineum germin-like protein 1 (CpGLP1) to understand its contribution to cell wall folding and desiccation tolerance. The analysis of the CpGLP1 sequence showed that this protein does not fit into the current GLP classification and forms a new group within the Linderniaceae. CpGLP1 transcripts accumulate in leaves in response to dehydration and ABA, and mannitol treatments transiently induce CpGLP1 transcript accumulation supporting the participation of CpGLP1 in desiccation-related processes. CpGLP1 protein from cell wall protein extracts followed transcript accumulation and protein preparations from bacteria overexpressing CpGLP1 showed SOD activity. In agreement with cell wall localization, CpGLP1 interacts with pectins which have not been reported for GLP proteins. Our data support a role for CpGLP1 in the ROS metabolism related to the control of cell wall plasticity during desiccation in C. plantagineum.

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Expression study of soybean germin-like gene family reveals a role ofGLP7gene in various abiotic stress tolerances

Cited by 15 publications

References 24 publications

Transcriptome analysis of bread wheat leaves in response to salt stress

Transcriptome analysis of bread wheat leaves in response to salt stress

Molecular evidence for the involvement of cotton GhGLP2, in enhanced resistance to Verticillium and Fusarium Wilts and oxidative stress

The dehydration- and ABA-inducible germin-like protein CpGLP1 from Craterostigma plantagineum has SOD activity and may contribute to cell wall integrity during desiccation

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