ExsE Is a Negative Regulator for T3SS Gene Expression in Vibrio alginolyticus

Liu, Jinxin; Lu, Shao-Yeh; Orfe, Lisa H.; Ren, Chen; Hu, Chaoqun; Call, Douglas R.; Avillan, Johannetsy J.; Zhao, Zhe

doi:10.3389/fcimb.2016.00177

Cited by 17 publications

(17 citation statements)

References 32 publications

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“…TLH is expressed by all clinical and environmental strains of V. parahaemolyticus (Bej et al, 1999 ) and it may have similar biological functions similar to these of the TDH (thermostable direct hemolysin) and TRH (TDH-related hemolysin) toxins, playing a key role in Vibrio infections (Wang R. et al, 2015 ). We also performed LDH release assay using FHM cells (Liu et al, 2016 ) to test the cytotoxicity activity (Figure 4 ). Cell death was observed after 3 h post-infection and up to 80% of the cells are lysed within 6 h (Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids

et al. 2017

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Vibrio spp. are the most common pathogens for animals reared in aquaculture. Vibrio campbellii, which is often involved in shrimp, fish and mollusks diseases, is widely distributed in the marine environment worldwide, but our knowledge about its pathogenesis and antimicrobial resistance is very limited. The existence of this knowledge gap is at least partially because that V. campbellii was originally classified as Vibrio harveyi, and the detailed information of its comparative genome analysis to other Vibrio spp. is currently lacking. In this study, the complete genome of a V. campbellii predominant strain, LMB29, was determined by MiSeq in conjunction with PacBio SMRT sequencing. This genome consists of two circular DNA chromosomes and four megaplasmids. Comparative genome analysis indicates that LMB29 shares a 96.66% similarity (average nucleotide identity) with the V. campbellii ATCC strain BAA-1116 based on a 75% AF (average fraction) calculations, and its functional profile is very similar to V. campbellii E1 and V. campbellii CAIM115. Both type III secretion system (T3SS) and type VI secretion system (T6SS), along with the tlh gene which encodes a thermolabile hemolysin, are present in LMB29 which may contribute to the bacterial pathogenesis. The virulence of this strain was experimental confirmed by performing a LDH assay on a fish cell infection model, and cell death was observed as early as within 3 h post infection. Thirty-seven antimicrobial resistance genes (>45% identity) were predicted in LMB29 which includes a novel rifampicin ADP ribosyltransferase, arr-9, in plasmid pLMB157. The gene arr-9 was predicted on a genomic island with horizontal transferable potentials which may facilitate the rifampicin resistance dissemination. Future researches are needed to explore the pathogenesis of V. campbellii LMB29, but the availability of this genome sequence will certainly aid as a basis for further analysis.

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Section: Resultsmentioning

confidence: 99%

Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids

et al. 2017

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“…Vibrio alginolyticus , a mildly halophilic Gram‐negative bacillus, is considered a critical opportunistic pathogen in mariculture (Cai, Cheng, Pang, Jian, & Wu, ; Chen, Cai, & Jian, ; Liu et al., ; Nishiki, Minami, Murakami, Hoai, & Fujiwara, ). V. alginolyticus is responsible for many epidemics of maricultured animals, such as coral reefs (Xie et al., ), shellfish (Mechri, Monastiri, Medhioub, Medhioub, & Aouni, ), fish (Cai, Cheng, Pang, Lu, & Jian, ; Zhao et al., ) and crustaceans (Gomez‐Gil et al., ; Jayaprakash, Pai, Philip, & Singh, ), which result in heavy economic losses V. alginolyticus also infects humans in warm periods, potentially causing otitis and wound infections (Campanelli, Sanchez‐Politta, & Saurat, ).…”

Section: Introductionmentioning

confidence: 99%

The role of rpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses

Huang

Guo

et al. 2019

Journal of Fish Diseases

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Vibrio alginolyticus is a leading aquatic pathogen, causing huge losses to aquaculture. rpoS has been proven to play a variety of important roles in stress response and virulence in several bacteria. In our previous study, upon treatment with Cu2+, Pb2+, Hg2+ and low pH, the expression levels of rpoS were downregulated as assessed by RNA‐seq, while impaired adhesion ability was observed, indicating that rpoS might play roles in the regulation of adhesion. In the present study, the RNAi technology was used to knockdown rpoS in V. alginolyticus. In comparison with wild‐type V. alginolyticus, RNAi‐treated bacteria showed significantly impaired abilities of adhesion, growth, haemolytic, biofilm production, movement and virulence. Meanwhile, alterations of temperature, salinity, pH and starvation starkly affected rpoS expression. The present data suggested that rpoS is a critical regulator of virulence in V. alginolyticus; in addition, rpoS regulates bacterial adhesion in response to temperature, pH and nutrient content changes. These are helpful to explore its pathogenic mechanism and provide reference for disease control.

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“…Furthermore, exsE , a type III secretion regulator, was predicated as the target of srvg23535 which indicates that srvg23535 is probably involved in the pathogenicity of V. alginolyticus . ExsE has been reported as a regulator of the expression of type III secretion system, which generally contributes to the adhesion in pathogens ( Rietsch et al, 2005 ; Erwin et al, 2012 ; Liu J. et al, 2016 ). In Vibrio species, including V. alginolyticus, exsE has been suggested to be a negative regulator of T3SS expression ( Erwin et al, 2012 ; Liu J. et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of a Novel Small RNA srvg23535 in Vibrio alginolyticus ZJ-T and Its Characterization With Phenotype MicroArray Technology

Deng

Liu

et al. 2018

Front. Microbiol.

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Small non-coding RNAs (sRNAs) are important modulators of gene expression and are involved in the pathogenesis and survival of prokaryotes. However, few studies have been conducted with Vibrio alginolyticus, which limits our ability to probe the regulation of virulence and environmental adaptation by sRNAs in this opportunistic pathogen. In this study, the sRNA candidate srvg23535 was identified in V. alginolyticus ZJ-T. The precise transcript end, secondary structure, and sequence conservation were determined. A srvg23535 null mutant was constructed and characterized by using Phenotype MicroArray (PM) technology. In silico target prediction was conducted by IntaRNA and TargetRNA2. Subsequently, a 107 nt transcript was validated with a sigma70 promoter at the 5′ end and a Rho-independent terminator at the 3′ end. The sRNA srvg23535 had four stem-loop structures and was conserved among Vibrio harveyi, Vibrio parahaemolyticus, and Vibrio splendidus. Deletion of srvg23535 in V. alginolyticus ZJ-T led to a weaker utilization of D-mannose, D-melibiose, lactulose, and inosine as carbon sources but stronger utilization of L-cysteine as nitrogen source. Moreover, the srvg2353 mutant showed stronger resistance to osmotic stress but weaker resistance to pH stress. Additionally, a total of 22 common targets were identified and several were related to the observed phenotype of the mutant. This study indicated that the novel sRNA, srvg23535, is conserved and restricted to Vibrio spp., affecting the utilization of several carbon and nitrogen sources and the response to osmotic and pH stress. These results extend our understanding of sRNA regulation in V. alginolyticus and provide a significant resource for the further study of the precise target mRNAs of srvg23535, which may provide targets for antibacterial therapeutic or attenuated vaccines against Vibrio spp.

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ExsE Is a Negative Regulator for T3SS Gene Expression in Vibrio alginolyticus

Cited by 17 publications

References 32 publications

Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids

Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids

The role of rpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses

Identification of a Novel Small RNA srvg23535 in Vibrio alginolyticus ZJ-T and Its Characterization With Phenotype MicroArray Technology

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