Extended focused image in microscopy by digital holography

Ferraro, Pietro; Grilli, Simonetta; Alfieri, Domenico; Nicola, S. De; Fińizio, Andrea; Pierattini, G.; Javidi, Bahram; Coppola, Giuseppe; Striano, V.

doi:10.1364/opex.13.006738

Cited by 235 publications

(103 citation statements)

References 15 publications

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“…A DHM hologram, on the other hand, does contain information of the full 3-D space of the specimen. This property is brought out to image the full volume of an object in extended focus imaging [169]. From a single hologram, a stack of amplitude images is calculated over a range of distances.…”

Section: Extended Focus Imagingmentioning

confidence: 99%

“…Microcantilever beams, bridges, and membranes are imaged by quantitative phase holography [22]. The multiwavelength optical phase unwrapping of phase images yields shape and deformation measurements with submicron precision over a many-micron range [151,169,190]. Various optical techniques, including digital holography, for characterization of MEMS devices are reviewed in Ref.…”

Section: Microscopy and Metrology Of Microstructuresmentioning

confidence: 99%

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Principles and techniques of digital holographic microscopy

2010

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Abstract. Digital holography is an emerging field of new paradigm in general imaging applications. We present a review of a subset of the research and development activities in digital holography, with emphasis on microscopy techniques and applications. First, the basic results from the general theory of holography, based on the scalar diffraction theory, are summarized, and a general description of the digital holographic microscopy process is given, including quantitative phase microscopy. Several numerical diffraction methods are described and compared, and a number of representative configurations used in digital holography are described, including off-axis Fresnel, Fourier, image plane, in-line, Gabor, and phase-shifting digital holographies. Then we survey numerical techniques that give rise to unique capabilities of digital holography, including suppression of dc and twin image terms, pixel resolution control, optical phase unwrapping, aberration compensation, and others. A survey is also given of representative application areas, including biomedical microscopy, particle field holography, micrometrology, and holographic tomography, as well as some of the special techniques, such as holography of total internal reflection, optical scanning holography, digital interference holography, and heterodyne holography. The review is intended for students and new researchers interested in developing new techniques and exploring new applications of digital holography. C 2010 Society of Photo-Optical Instrumentation Engineers.

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Section: Extended Focus Imagingmentioning

confidence: 99%

Section: Microscopy and Metrology Of Microstructuresmentioning

confidence: 99%

Principles and techniques of digital holographic microscopy

2010

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“…Various cellular parameters can be visualized and calculated from the particular hologram, including individual cell area, thickness, volume, and population confluence and cell counts [4][5][6][7][8][9][10]. One of the advantages of studying cells with DH microscopy is that they can be grown and analyzed in their normal growth medium during the entire study.…”

Section: Introductionmentioning

confidence: 99%

Holography: The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

El-Schich¹,

Kamlund²,

Janicke³

et al. 2017

Holographic Materials and Optical Systems

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Digital holographic (DH) microscopy is a digital high-resolution holographic imaging technique with the capacity of quantification of cellular conditions without any staining or labeling of cells. The unique measurable parameters are the cell number, cell area, thickness, and volume, which can be coupled to proliferation, migration, cell cycle analysis, viability, and cell death. The technique is cell friendly, fast and simple to use and has unique imaging capabilities for time-lapse investigations on both the single cell and the cell-population levels. The interest for analyzing specifically cell volume changes with DH microscopy, resulting from cytotoxic treatments, drug response, or apoptosis events has recently increased in popularity. We and others have used DH microscopy showing that the technique has the sensitivity to distinguish between different cells and treatments. Recently, DH microscopy has been used for cellular diagnosis in the clinic, providing support for using the concept of DH, e.g., screening of malaria infection of red blood cells (RBC), cervix cancer screening, and sperm quality. Because of its quick and label-free sample handling, DH microscopy will be an important tool in the future for personalized medicine investigations, determining the optimal therapeutic concentration for both different cancer types and individual treatments.

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“…Digital holographic microscopy (DHM) is an optical technique useful to retrieve quantitative information of microscopic objects with subwavelength axial accuracy (Haddad et al, 1992;Zhang-Yamaguchi, 1998;Pedrini et al, 1999;Ferraro et al, 2005). An important feature of DHM is its non-invasive character that allows the appropriate study of microorganisms in vivo (Car et al, 2004;Marquet et al, 2005;Rappaz et al, 2005Javidi et al, 2006;Charrière et al, 2006).…”

Section: Introductionmentioning

confidence: 99%