Extended recombinant bacterial ghost system

Lubitz, Werner; Witte, Angela; Eko, Francis O.; Kamal, M.; Jechlinger, Wolfgang; Brand, Edith; Marchart, J.; Haidinger, W.; Huter, Veronika; Felnerova, Diana; Stralis-Alves, N.; Lechleitner, Sonja; Melzer, Helen; Szostak, Michael P.; Resch, Stephanie; Mader, H.; Kuen, Beatrix; Mayr, B.; Mayrhofer, Peter; Geretschläger, R.; Haslberger, Alexander G.; Hensel, Andreas

doi:10.1016/s0168-1656(99)00144-3

Cited by 60 publications

(41 citation statements)

References 26 publications

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“…Bacterial ghosts made from gram-negative bacteria like Escherichia coli reduce some of these risks. They are nonliving and can be used as antigen delivery particles [4,5]. These ghosts are empty cell envelopes made by protein-Emediated lysis which have retained almost all morphological and structural features of the natural cell.…”

Section: Mucosal Immunizationmentioning

confidence: 99%

See 1 more Smart Citation

Mucosal vaccine delivery of antigens tightly bound to an adjuvant particle made from food-grade bacteria

Roosmalen¹,

Kanninga²,

Khattabi³

et al. 2006

Methods

106

102

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Section: Mucosal Immunizationmentioning

confidence: 99%

“…Subsequently, the cells were diluted 100-fold in 1 l of fresh pre-warmed (30°C) GM17Cm 5 . At a cell density OD 600 of 0.5, expression of the PA-fusion protein was induced by addition of 1 ml of cell-free nisin-containing supernatant produced from an overnight culture of L. lactis NZ9700.…”

Section: Overexpression Of Pa-fusion Proteins In L Lactismentioning

confidence: 99%

Mucosal vaccine delivery of antigens tightly bound to an adjuvant particle made from food-grade bacteria

Roosmalen¹,

Kanninga²,

Khattabi³

et al. 2006

Methods

106

102

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“…Upon discovery of the remarkable features of protein E-mediated lysis in E. coli, the principle of E-lysis could be shown with other Gram-negative bacteria 24,25 but not for Gram-positive bacteria. 26 So far, BGs of numerous Gram-negative strains (different E. coli strains, Salmonella typhimurium, Salmonella enteritidis, Klebsiella pneumoniae, Bordetella bronchiseptica, Heliobacter pylori, Vibrio cholerae, Actinobacillus pleuropneumoniae, Haemophilus influenzae, Mannheimia haemolytica, Pasteurella multocida, Pseudomonas aeruginosa, Pseudomonas putida, Ralstonia eutropha, Pectobacterium cypripedii and others) have been generated successfully.…”

Section: Introductionmentioning

confidence: 99%

The bacterial ghost platform system

Langemann¹,

et al. 2010

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The Bacterial Ghost (BG) platform technology is an innovative system for vaccine, drug or active substance delivery and for technical applications in white biotechnology. BGs are cell envelopes derived from Gram-negative bacteria. BGs are devoid of all cytoplasmic content but have a preserved cellular morphology including all cell surface structures. Using BGs as delivery vehicles for subunit or DNA-vaccines the particle structure and surface properties of BGs are targeting the carrier itself to primary antigenpresenting cells. Furthermore, BGs exhibit intrinsic adjuvant properties and trigger an enhanced humoral and cellular immune response to the target antigen. Multiple antigens of the native BG envelope and recombinant protein or DNA antigens can be combined in a single type of BG. Antigens can be presented on the inner or outer membrane of the BG as well as in the periplasm that is sealed during BG formation. Drugs or supplements can also be loaded to the internal lumen or periplasmic space of the carrier. BGs are produced by batch fermentation with subsequent product recovery and purification via tangential flow filtration. For safety reasons all residual bacterial DNA is inactivated during the BG production process by the use of staphylococcal nuclease A and/or the treatment with β-propiolactone. After purification BGs can be stored long-term at ambient room temperature as lyophilized product. The production cycle from the inoculation of the pre-culture to the purified BG concentrate ready for lyophilization does not take longer than a day and thus meets modern criteria of rapid vaccine production rather than keeping large stocks of vaccines. The broad spectrum of possible applications in combination with the comparably low production costs make the BG platform technology a safe and sophisticated product for the targeted delivery of vaccines and active agents as well as carrier of immobilized enzymes for applications in white biotechnology.

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“…That might be due to the force happened as the result of the existence of the first pore. When first pore is being opened; it imbalance the internal pressure lead to getting rid of the cytoplasm contents and the rest of the E lysis protein so there is no chance to form another pore [29].…”

Section: Amino Acids Sequence: Mvrwtlwdtlafllllslllpsl-limfipstfkrpvsmentioning

confidence: 99%

Lysozymes, Proteinase K, Bacteriophage E Lysis Proteins, and some Chemical Compounds for Microbial Ghosts Preparation: a Review and Food for Thought

Amara¹

2016

SOJB

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Extended recombinant bacterial ghost system

Cited by 60 publications

References 26 publications

Mucosal vaccine delivery of antigens tightly bound to an adjuvant particle made from food-grade bacteria

Mucosal vaccine delivery of antigens tightly bound to an adjuvant particle made from food-grade bacteria

The bacterial ghost platform system

Lysozymes, Proteinase K, Bacteriophage E Lysis Proteins, and some Chemical Compounds for Microbial Ghosts Preparation: a Review and Food for Thought

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