Extended Spectrum Beta- Lactamase- Producing Uropathogenic Escherichia coli in Pregnant Women Diagnosed With Urinary Tract Infections in South-Western Nigeria

Abstract

Extended spectrum beta-lactamase (ESBL)-producing uropathogenic Escherichia coli (UPEC) in symptomatic pregnant women with confirmed urinary tract infections in Southwest. Nigeria was reported. Susceptibility of UPEC isolates to β-lactam and other classes of antibiotics was determined by the Kirby-Bauer's disc diffusion method on Mueller-Hinton agar plate. Detection of plasmid DNA in multiple antibiotic resistant isolates was carried out by alkali lysis (TENS) method. Extended-spectrum-β-lactamase (ESBL) production was determined by double disk synergy test (DDST). Isolates that were positive to ESBL were mated with non-ESBL-producing E. coli and other enterics in a conjugation experiment. Transfer of ESBL-enzyme and other resistance phenotypes in the transconjugants was investigated by DDST. Data obtained were statistically analyzed using SPSS 17. Greater percentage of the isolates were multiple antibiotic resistant (MAR). Sixty-nine (26.1 %) of UPEC were ESBL producers. Some of the ESBL producers transferred ESBL-enzyme and other resistance determinants to the recipients. Large size plasmid DNA of molecular weight (23.13-33.04 kb) was detected in some representative MAR isolates.

Dissemination of Multi-Drug Resistance in Escherichia coli Isolated from Apparently Healthy School Children in Ondo State, Nigeria

“…Moreover, some isolates from male and female were resistant to 10 out of 11 antibiotics. This observation is similar to the work carried out by Oluduro et al [25]. According to the work, high prevalence of MDR strains was obtained.…”

Extended‐spectrum beta‐lactamase (ESBL)‐producingEnterobacteriaceaeand urinary tract infections in pregnant/postpartum women: A systematic review and meta‐analysis

“…The prevalence of ESBL‐positive E coli isolates was higher in patients with AB (33%, 95% CI 21, 45%) than in pregnant women diagnosed by symptomatic UTI (16%, 95% CI 8, 24%). The prevalence estimate was 6% (95% CI 0, 12%) in three studies with high quality and it was 20% (95% CI 11, 29%) in seven studies with low quality . Considering the results of investigation for reporting bias, the Begg's ( Z = 1.43; P = .15) and Egger's tests (bias = −2.21; P = .07) results demonstrated that this bias is ignorable.…”

“…Ten studies (two from Africa, four from Asia and four European studies) investigated ESBL production by E coli isolates among pregnant women with UTIs 18,47,[60][61][62][63][64][65] ; Tables 3 and 4. The pooled prevalence of ESBL-P E coli isolates was 17% (95% CI 9, 24%), I 2 = 88.27%; Figure 5.…”

“…The prevalence estimate was 6% (95% CI 0, 12%) in three studies with high quality 47,60,61 and it was 20% (95% CI 11, 29%) in seven studies with low quality. 18,[62][63][64][65] Considering the results of investigation for reporting bias, the Begg's (Z = 1.43; P = .15) and Egger's tests (bias = −2.21; P = .07) results demonstrated that this bias is ignorable. Sensitivity analysis also showed that none of the individual studies had significant influence on the overall estimate.…”

Maternal colonization or infection with extended-spectrum beta-lactamase-producing Enterobacteriaceae in Africa: A systematic review and meta-analysis

“…Five studies (Nelson et al, 2014;Olufunke et al, 2014;Aljabri et al, 2010;Bebell et al, 2017;Tito et al, 2017), detected ESBL production using the double disc synergy test (DDST).…”