Extenders with vitamins C and E applied to Rhamdia quelen sperm cryopreservation / Extensores com vitaminas C e E aplicados à criopreservação de esperma de Rhamdia quelen

Abstract

We performed this experiment to evaluate the effects of adding vitamins C and E on extenders for sperm cryopreservation of Rhamdia quelen over spermatic mobility after thawing. At cryopreservation, sperm samples were diluted in a proportion of 1:3 (v/v), following pre-freezing in nitrogen steam and subsequent immersion in liquid nitrogen. The diluents were composed by 5% milk powder, 5% glucose, 10% methanol and different levels of vitamin. Three sperm cryopreservation tests were carried out with (1) diluent containing 0.0; 4.0; 6.5; 9.0 and 11.5 mg of vitamin C mL -1 , (2) diluent containing 0.0; 2.0; 4.0; 6.0 and 8.0 mg of vitamin E mL -1 ; (3) diluent containing 0.0; 4.0 + 2.0; 6.5 + 4.0; 9.0 + 6.0 and 11.5 + 8.0 mg of vitamin C mL -1 plus vitamin E mL -1 , respectively. The spermatic motility rate, spermatic curvilinear velocity, average path and straight line velocities were measured in thawed semen by CASA. Data were submitted to ANOVA and Duncan´s test at 5% of significance. After thawing the effect (P<0.05) of vitamin C was observed only for sperm motility, with higher values (38.2±20.7%) on solution containing 4.0 mg of vitamin C mL -1 . The concomitant addition of both vitamins influenced (P<0.05) only the curvilinear velocity, reducing the velocity at any concentration. In conclusion, diluents with 4.0 mg vitamin C mL -1 to cryopreservation of the silver catfish semen improve the sperm quality after thawing, and the use of diluents with vitamin E or both vitamins are not recommended because do not ensure the cells protection.

Effects of vitamins C and E on semen quality and reproductive hormones in yellow mystus (Hemibagrus spilopterus)

“…The study results suggested that the supplementation with Vit C, Vit E or their combination positively affected sperm quality (Table 2). Similarly, Xu et al (2015) observed increased sperm motility and viability, indicating improved reproductive performance in turbot (Scophthalmus maximus), rainbow trout (Canyurt and Akhan, 2008), fertilization in Atlantic salmon (Figueroa et al, 2018), and in Rhamdia quelen (Xavier et al, 2021). According to the current results, when given in combination, Vit C and Vit E were likely to synergistically influence hormone synthesis and hormone production, including prostaglandin synthesis.…”