Extending the Dormant Bud Cryopreservation Method to New Tree Species

Jenderek, Maria M.; Ambruzs, Barbara D.; Tanner, Johanna; Holman, G.; Ledbetter, Craig A.; Postman, Joseph D.; Ellis, David; Leslie, Charles A.

doi:10.17660/actahortic.2014.1039.16

Cited by 8 publications

(2 citation statements)

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“…This protocol is generally used with Malus (e.g. Volk et al 2008;Grout et al 2011;Lambardi et al 2011;Höfer 2015;Höfer and Hanke 2017) and also applied for Salix (Towill and Widrlechner 2004;Jenderek et al 2014) Fraxinus (Volk et al 2009) Juglans, Prunus, (Jenderek et al 2014) and Vaccinium . Protocols where recovery of buds is done via in vitro culture have been developed e.g.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

Rantala

Kaseva

Karhu

et al. 2019

Plant Cell Tiss Organ Cult

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Cryopreservation of dormant buds can be a feasible method for long term preservation of clonally propagated woody plants.In the present study, dormant buds of blackcurrant (Ribes nigrum L) cultivar Mortti were cryopreserved. Twig segments from greenhouse and field grown plants were cooled at their natural moisture content at the rate of 0.17 °C min −1 from zero to −38 °C, immersed in liquid nitrogen and stored in liquid nitrogen vapor. The post-cryopreservation regrowth of buds was evaluated in vitro and viability of recovered plants was tested in vivo. The estimated recovery rate for buds thawed after 1-6 days in cryostorage was 86% and 66% for outdoor and greenhouse grown buds, respectively. Microplants adequate in size and quality grew successfully in vivo and plants produced berries in field. After 4 years in cryostorage, the estimated recovery rate for outdoor buds was 58%. According to results from 1 to 6 days cryostorage, the tested dormant bud protocol turned to be applicable for cryopreservation of the blackcurrant cultivar Mortti. However, concerning the long-term preservation the decline of recovery is important to consider in the future experiments. Key messageThe cryopreservation of dormant buds combined with in vitro recovery provides a feasible tool for conservation of plant genetic resources of blackcurrant.

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Section: Introductionmentioning

confidence: 99%

Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

Rantala

Kaseva

Karhu

et al. 2019

Plant Cell Tiss Organ Cult

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“…The dehydration of explants intended for cryopreservation is mainly used with seeds, zygotic embryos, or embryonic axes extracted from seeds followed by direct immersion in liquid nitrogen for rapid cooling, except for oily seeds (e.g., Arachis hypogea ), which require a slow pre-cooling phase in a programmable cooler before cryopreservation [ 130 ] and a slow seed imbibition phase over water [ 133 ]. The natural cold acclimatization of twigs in combination with dehydration is also a key element for dormant bud cryopreservation, which usually requires controlled-rate cooling [ 134 , 135 ]. At the Millennium Seed Bank of the Royal Botanic Gardens, Kew, desiccation-tolerant, orthodox seeds of wild species with short lifespans under standard long-term conservation conditions (−20 °C) are dried at about 32 ± 3% RH at 18 °C and are then stored in the vapor phase of liquid nitrogen [ 94 ].…”

Section: Description Of Ex Situ Germplasm Conservation Methods and Their Strengths And Weaknessesmentioning

confidence: 99%

A Critical Review of the Current Global Ex Situ Conservation System for Plant Agrobiodiversity. I. History of the Development of the Global System in the Context of the Political/Legal Framework and Its Major Conservation Components

Engels

Ebert

2021

Plants

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The history of ex situ conservation is relatively short, not more than a century old. During the middle of last century, triggered by the realization that genetic erosion was threatening the existing landraces and wild relatives of the major food crops, global efforts to collect and conserve the genetic diversity of these threatened resources were initiated, predominantly orchestrated by FAO. National and international genebanks were established to store and maintain germplasm materials, conservation methodologies were created, standards developed, and coordinating efforts were put in place to ensure effective and efficient approaches and collaboration. In the spontaneously developing global conservation system, plant breeders played an important role, aiming at the availability of genetic diversity in their breeding work. Furthermore, long-term conservation and the safety of the collected materials were the other two overriding criteria that led to the emerging international network of ex situ base collections. The political framework for the conservation of plant genetic resources finds its roots in the International Undertaking of the FAO and became ‘turbulent rapid’ with the conclusion of the Convention on Biological Diversity. This paper reviews the history of the global ex situ conservation system with a focus on the international network of base collections. It assesses the major ex situ conservation approaches and methods with their strengths and weaknesses with respect to the global conservation system and highlights the importance of combining in situ and ex situ conservation.

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Cryopreserved storage of clonal germplasm in the USDA National Plant Germplasm System

Jenderek

Reed

2017

In Vitro Cell.Dev.Biol.-Plant

101

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Extending the Dormant Bud Cryopreservation Method to New Tree Species

Cited by 8 publications

References 0 publications

Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

A Critical Review of the Current Global Ex Situ Conservation System for Plant Agrobiodiversity. I. History of the Development of the Global System in the Context of the Political/Legal Framework and Its Major Conservation Components

Cryopreserved storage of clonal germplasm in the USDA National Plant Germplasm System

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