Extension of the diffraction resolution of crystals

Schick, Brian; Jurnak, Frances

doi:10.1107/s0907444994001976

Cited by 22 publications

(15 citation statements)

References 12 publications

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“…Two reports have appeared in which increased diffractivity is produced by a solute-replacement protocol (Yennawar, Yennawar & Farber, 1994;Schick & Jurnak, 1994). A common denominator of these and the present report is a decrease in the activity of water in the external solution and thus within the crystal.…”

Section: Discussionsupporting

confidence: 58%

Enhanced Diffractivity of Phosphoglucomutase Crystals. Use of an Alternative Cryocrystallographic Procedure

Baranidharan²,

Y³

1997

Acta Cryst D

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A continuous procedure for replacing the 2M (NH4)2SO4 within crystals of rabbit muscle phosphoglucomutase by 55% polyethylene glycol 600 (PEG 600) is described. The success rate (absence of fracturing) approaches 100% in spite of the fragility of the crystals. The procedure is based on the use of a biphasic PEG/salt mixture in conjunction with a flow cell that allows several crystals to be treated at once. The holdup volume of the cell is small and its design minimizes concentration gradients. Cooling treated crystals to 253 K elicits a substantial increase in diffractivity that allows data collection to be extended from about 2.75 to about 2.35 ]k. However, neither the dimensions of the unit cell nor the structure of the asymmetric unit are significantly altered. Comparisons are made between data sets collected at 289 K using crystals in 2.2 M (NH4)2SO4 and at 253 K using crystals in 55% PEG 600. Models of the enzyme refined against one or the other data sets are also compared. These comparisons suggest that, at most, only a minor fraction of the increased diffractivity is caused by lowered atomic B values. The increased diffractivity also is not the result of a simple temperature effect since at high-salt concentration the same cooling protocol fails to significantly increase diffractivity. A decrease in mosaic spread is considered as a possible explanation for the increased diffractivity.

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Section: Discussionsupporting

confidence: 58%

Enhanced Diffractivity of Phosphoglucomutase Crystals. Use of an Alternative Cryocrystallographic Procedure

Baranidharan²,

Y³

1997

Acta Cryst D

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“…Further, post-crystallization methods to enhance crystal quality-dehydration, annealing, soaking, and other treatments-became popular and cured many "poor" crystals (Heras and Martin, 2005). Here as well, observations on crystals of proteins from the translation machinery-a GlnRS and EF-Tu-where among the first that opened the field (Rould et al, 1991;Schick and Jurnak, 1994). Second, the access to strong synchrotron X-ray light sources increased sharply with the building of new third generation facilities worldwide.…”

Section: Better and Faster Methods For Structure Determinationmentioning

confidence: 99%

Biocrystallography: Past, present, future

Giegé

Sauter

2010

HFSP Journal

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“…In addition, sometimes the soak in the cryo-solution, especially PEGs, causes dehydration which results in increased order (e.gs. Schick & Jurnak, 1994;Esnouf et al, 1998).…”

Section: Higher Resolutionmentioning

confidence: 99%

Cool data: quantity AND quality

Garman

1999

Acta Crystallogr D Biol Cryst

155

142

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The use of cryo-techniques in macromolecular crystallography has increased enormously over the last eight years and has become a vital part of modern X-ray data-collection methods. This paper presents some reasons for the rise in popularity of cryo-techniques and a brief outline of the basic methods, followed by a detailed discussion of factors to be considered when trying to optimize both the quantity and quality of the data collected. As more experimenters at synchrotrons observe significant radiation damage to crystals held near 100 K, the available options for further prolonging crystal lifetime and extending the techniques become worth investigating. Some possibilities and parameters to be considered are presented, although these must remain speculative until more experimental data are available.

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Extension of the diffraction resolution of crystals

Cited by 22 publications

References 12 publications

Enhanced Diffractivity of Phosphoglucomutase Crystals. Use of an Alternative Cryocrystallographic Procedure

Enhanced Diffractivity of Phosphoglucomutase Crystals. Use of an Alternative Cryocrystallographic Procedure

Biocrystallography: Past, present, future

Cool data: quantity AND quality

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