Extensive genetic heterogeneity in the neuroblastoma cell line NB(TU)1

Inoue, Akira; Yokomori, Kinji; Tanabe, Hideyuki; Mizusawa, Hiroshi; Sofuni, Toshio; Hayashi, Yasuhide; Tsuchida, Yoshiaki; Shimatake, Hiroyuki

doi:10.1002/(sici)1097-0215(19970917)72:6<1070::aid-ijc23>3.0.co;2-7

Cited by 10 publications

(4 citation statements)

References 13 publications

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“…Four of them (SCMC‐N2, SCMC‐N3, SCMC‐N4, and SCMC‐N5) were established in our laboratory (Kong et al, 1997; Yang et al, 2000). Six SJNB cell lines (SJNB‐1, SJNB‐2, SJNB‐4, SJNB‐5, SJNB‐7, SJNB‐8) and NBTU1 (Inoue et al, 1997) were generous gifts from Dr. A. T. Look and Dr. A. Inoue, respectively. The other cell lines were obtained from the Japanese Cancer Resource Cell Bank (http://cellbank.nihs.go.jp/).…”

Section: Methodsmentioning

confidence: 99%

Gene expression profiling and identification of novel prognostic marker genes in neuroblastoma

Takita

Ishii

Tsutsumi

et al. 2004

Genes Chromosomes & Cancer

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To investigate the various genetic characteristics of and differences between early- and advanced-stage neuroblastoma (NB) and to identify candidate genes involved in NB progression, we performed DNA microarray analysis on 20 primary tumors. Two-way clustering analysis based on the expression pattern of approximately 500 of 1,700 genes revealed genetic subgroups in these NB tumors. Although 9 of the 13 early-stage tumors (69%) and 4 of the 6 advanced-stage tumors (67%) were classified as being in the same cluster, the remaining tumors showed different expression profiles. This indicates that both the early- and advanced-stage tumors were heterogeneous. Based on the microarray data, we identified the BIRC, CDKN2D, and SMARCD3 genes as those that are predominantly expressed in either the early or the advanced stage of NB. These genes have been reported to be associated with apoptosis, cell cycles, and the transcriptional activator, respectively. To better assess the prognostic value of the expression of these genes in NB, real-time polymerase chain reaction was carried out on 50 primary tumors. The expression of both the BIRC3 and CDKN2D genes was significantly higher in the early-stage group than in the advanced-stage group (P = 0.002 and 0.003, respectively), whereas the expression of the SMARCD3 gene was significantly reduced in the early-stage group (P = 0.02). Therefore, the BIRC, CDKN2D, and SMARCD3 genes are possible candidates for being novel prognostic markers for NB.

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Section: Methodsmentioning

confidence: 99%

Gene expression profiling and identification of novel prognostic marker genes in neuroblastoma

Takita

Ishii

Tsutsumi

et al. 2004

Genes Chromosomes & Cancer

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“…Four of them (SCMC-N2, SCMC-N3, SCMC-N4 and SCMC-N5) were established in our laboratory (Kong et al, 1997a;Yang et al, 2000). Six of them (SJNB-1, SJNB-2, SJNB-4, SJNB-5, SJNB-7, SJNB-8) and NBTU1 (Inoue et al, 1997) were a generous gift from Dr AT Look and Dr A Inoue, respectively. The other cell lines were obtained from the Japanese Cancer Resource Cell Bank (http://cellbank.nihs.go.jp/).…”

Section: Tumor Cell Linesmentioning

confidence: 99%

Allelic imbalance on chromosome 2q and alterations of the caspase 8 gene in neuroblastoma

et al. 2001

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We previously reported a high incidence of loss of heterozygosity (LOH) on chromosome 2q33 in neuroblastoma (NB), observed in various types of human cancers including lung cancer, head and neck cancer and follicular thyroid carcinoma. To better elucidate the role of chromosome 2q aberrations in NB, we examined common allelic imbalance (AI) regions on chromosome 2q in 82 NB patients using 10 polymorphic microsatellite markers. AI on 2q was detected in 26 (32%) of 82 NB cases. There was a distinct common AI region between the D2S115 and D2S307 markers on 2q33. The distance between these markers was about 2.0 cM. Recently, the caspase 8 and caspase 10 genes, both of which encode cystein protease, were mapped to chromosome 2q33. Since the common AI region on 2q33 includes the caspase 8 and caspase 10 genes, the alterations of these genes were examined further. Absent or reduced expression of caspase 8 and caspase 10 were found in 19 (70%) of 27 and two (7%) of 27 NB cell lines by reverse transcription-polymerase chain reaction, respectively. A missense mutation was detected at codon 96, GCT (Alanine) to GTT (Valine), of the caspase 8 gene in one of the NB cell lines lacking caspase 8 expression. Thirteen (68%) of 19 cell lines lacking caspase 8 expression displayed methylation of the CpG island of the caspase 8 gene, whereas only one (13%) of eight cell lines with caspase 8 expression showed caspase 8 methylation (P=0.031). Furthermore, there was a signi®cant association between AI at 2q33 and loss of caspase 8 expression (P=0.026). These results indicated that there was a tumor suppressor gene in the common AI region on chromosome 2q33 involved in the pathogenesis of a subset of NB. It is possible that the caspase 8 gene is one of the candidate tumor suppressor genes for NB and inactivation of this gene plays an important role in the tumorigenesis of NB through mainly its methylation. Oncogene (2001) 20, 4424 ± 4432.

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“…They did not attach properly with the spindle during the segregation process in anaphase (Fenech 2007;Fenech and Bonassi 2011). Therefore, both clastogenic and aneugenic effects can be determined with the MN assay which may reflect genomic instability (Inoue et al 1997;Albertini et al 2000;Kirsch-Volders et al 2011). In our study ZnO NPs also increased the MN frequency.…”

Section: Discussionmentioning

confidence: 56%

In vitro genotoxic effects of ZnO nanomaterials in human peripheral lymphocytes

et al. 2013

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In this study, possible genotoxic effects of zinc oxide (ZnO) nanoparticles were investigated in cultured human peripheral lymphocytes by using chromosome aberrations and micronucleus assays (MN). For this purpose, the cells were treated with ZnO (1, 2, 5, 10, 15 and 20 lg/mL) for 24 and 48 h. In this research, four types of chromosome aberrations were observed as chromatid and chromosome breaks, fragment and dicentric chromosomes. ZnO induced significant increase of the ratio of chromosomal aberrations as well as percentage of abnormal cells at concentrations of 1, 5, 10 and 20 lg/mL in 24 h treatments. In 48 h treatments, while ZnO nanomaterials induced significant increase of the percentage of abnormal cells only at a concentration of 10 lg/mL, and of chromosome aberration per cell in comparison to the control at concentrations of 5 and 10 lg/mL. On the other hand, this material significantly increased the micronuclei frequency (MN) at concentrations of 10 and 15 lg/mL in comparison to the control. Cytokinesis-block proliferation index was not affected by ZnO treatments. It also decreased the mitotic index in all concentrations at 24 h but not at 48 h. The present results indicate that ZnO nanoparticles are clastogenic, mutagenic and cytotoxic to human lymphocytes in vitro at specific concentrations and time periods.

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Extensive genetic heterogeneity in the neuroblastoma cell line NB(TU)1

Cited by 10 publications

References 13 publications

Gene expression profiling and identification of novel prognostic marker genes in neuroblastoma

Gene expression profiling and identification of novel prognostic marker genes in neuroblastoma

Allelic imbalance on chromosome 2q and alterations of the caspase 8 gene in neuroblastoma

In vitro genotoxic effects of ZnO nanomaterials in human peripheral lymphocytes

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