External Volume Expansion Adjusted Adipose Stem Cell by Shifting the Ratio of Fibronectin to Laminin

Abstract

External volume expansion (EVE) is an effective method of adipose tissue regeneration. However, it remains unclear how EVE induces adipose tissue regeneration. In this study, we developed EVE devices to generate expanded prefabricated adipose tissue (EPAT) in rats and investigated cell proliferation, adipogenesis, and the expression of extracellular matrix (ECM) proteins during the 12 weeks suction. In addition, EPAT-generated decellularized adipose tissue (DAT) was used to assess the role of ECM proteins in cell proliferation and differentiation. Matrix deposition was significantly increased after EVE suction, with fibronectin and laminin showing the most dramatic changes. Fibronectin expression peaked during weeks 1-4, when Ki67 cells in EPAT peaked. Laminin expression peaked during weeks 8-12, when peroxisome proliferator-activated receptor-g expression also peaked. In vitro, adipose-derived stem cells (ASCs) displayed a higher proliferation rate in week 1 DAT, when fibronectin expression was highest, whereas ASC adipogenesis was significantly higher on week 12 DAT, when laminin expression was abundant. These results showed that EVE device enhanced ECM deposition, which is closely related to cell proliferation and differentiation.

The Application and Mechanism of Action of External Volume Expansion in Soft Tissue Regeneration

“…81 EVE suction for 12 weeks gradually increased the expression of laminin and of the adipogenic genes, PPARg and CEBPa, with peaks occurring simultaneously. 54 Moreover, the adipogenicity of ADSCs reseeded onto 12-week EPAT-DAT in vitro was greater than the adipogenicity of ADSCs reseeded onto 1-week EPAT-DAT and 1-week control-DAT. These results suggested a strong relationship between laminin and adipogenic expression (Fig.…”

“…37 In addition, the expression of fibronectin and the number of proliferating cells were increased at week 1 in EPAT, providing further evidence that EVE is involved in inducing ADSC proliferation. 54 In vitro assays showed that the proliferation rate of ADSCs in EPAT-generated decellularized adipose tissue (DAT) was higher at 1 week than at other time points. These findings confirmed that EVE-enhanced secretion of fibronectin regulates ADSC proliferation.…”

Cell Communications among Microorganisms, Plants, and Animals: Origin, Evolution and Interplays

“…In animals, cells are in contact with each other not only directly through these adhesion proteins but also indirectly via the extracellular matrix, ECM, kind of an extracellular "glue," which includes proteins such as collagens, fibronectins, or laminins [89][90][91]. Various adhesion proteins such as cadherins, integrins, CAM (Cell Adhesion Molecules), selectins, etc., participate in these direct or indirect contacts between cell surfaces [92][93][94].…”

Cell Communications among Microorganisms, Plants, and Animals: Origin, Evolution and Interplays

“…In animals, cells are in contact with each other not only directly through these adhesion proteins but also indirectly via a kind of extracellular "glue" the extracellular matrix, ECM, which includes proteins such as collagens, fibronectins or laminins [96][97][98]. In plants, many different hormones also exist, and they act at a very short distance, such as ethylene, or a more considerable distance such as auxin, gibberellins, and various phyto-oestrogens.…”