Extracellular acidic pH-activated, outward rectifying chloride currents can be regulated by reactive oxygen species in human THP-1 monocytes

Fu, Zhijie; Li, Xuezhong; Wang, Qirong; Shi, Li; Zhang, Liqiang; Pan, X L

doi:10.1016/j.bbrc.2013.01.090

Cited by 14 publications

(19 citation statements)

References 19 publications

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“…While it is possible that the acid-induced Cl − current resulted from enhanced activation of the constitutive Cl − current, it exhibited striking similarity to a group of previously discovered acid-induced Cl − currents [1], [8], [19], [40], [41], [47], [48], [53], [64], [65], [75], [78]. To further compare the MDCT currents, we studied the effects of NPPB (100 μM), DIDS (100 μM), and FFA (100 μM) on the acid-induced Cl − current (Fig.…”

Section: Resultsmentioning

confidence: 54%

“…However, many Cl − currents are not only anion permeable [1], [6], [8], [16], [17], [19], [41], [46], [53], [57], [59], [78], but are permeable to negatively charged molecules such as glutamate − [6], [59] and CH 3 SO 3 − [28], [31], [68].…”

Section: Resultsmentioning

confidence: 99%

“…Acid-induced outwardly rectifying Cl − currents have been found in neurons, cardiac myocytes, blood cells, and epithelial cells [1], [8], [19], [40], [41], [47], [48], [53], [64], [65], [75], [78]. These studies primarily examined biophysical properties, and presently little is known about the biological role or molecular entity of these currents.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of constitutive and acid-induced outwardly rectifying chloride currents in immortalized mouse distal tubular cells

Valinsky

Touyz

Shrier

2017

Biochimica et Biophysica Acta (BBA) - General Subjects

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Thiazides block Na+ reabsorption while enhancing Ca2+ reabsorption in the kidney. As previously demonstrated in immortalized mouse distal convoluted tubule (MDCT) cells, chlorothiazide application induced a robust plasma membrane hyperpolarization, which increased Ca2+ uptake. This essential thiazide-induced hyperpolarization was prevented by the Cl− channel inhibitor 5-Nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), implicating NPPB-sensitive Cl− channels, however the nature of these Cl− channels has been rarely described in the literature. Here we show that MDCT cells express a dominant, outwardly rectifying Cl− current at extracellular pH 7.4. This constitutive Cl− current was more permeable to larger anions (Eisenman sequence I; I− > Br− ≥ Cl−) and was substantially inhibited by > 100 mM [Ca2+]o, which distinguished it from ClC-K2/barttin. Moreover, the constitutive Cl− current was blocked by NPPB, along with other Cl− channel inhibitors (4,4′-diisothiocyanatostilbene-2,2′-disulfonate, DIDS; flufenamic acid, FFA). Subjecting the MDCT cells to an acidic extracellular solution (pH < 5.5) induced a substantially larger outwardly rectifying NPPB-sensitive Cl− current. This acid-induced Cl− current was also anion permeable (I− > Br− > Cl−), but was distinguished from the constitutive Cl− current by its rectification characteristics, ion sensitivities, and response to FFA. In addition, we have identified similar outwardly rectifying and acid-sensitive currents in immortalized cells from the inner medullary collecting duct (mIMCD-3 cells). Expression of an acid-induced Cl− current would be particularly relevant in the acidic IMCD (pH < 5.5). To our knowledge, the properties of these Cl− currents are unique and provide the mechanisms to account for the Cl− efflux previously speculated to be present in MDCT cells.

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Section: Resultsmentioning

confidence: 54%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of constitutive and acid-induced outwardly rectifying chloride currents in immortalized mouse distal tubular cells

Valinsky

Touyz

Shrier

2017

Biochimica et Biophysica Acta (BBA) - General Subjects

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“…These biophysical and pharmacological properties of neuronal acid-activated currents are phenotypically identical to those of ASOR anion channel expressing in a variety of non-neuronal cells. [1][2][3][4][5][6][7][8][9][10] Since our previous study 11 showed high sensitivity of the human epithelial ASOR channel currents to temperature, we next observed the effects of continuous changes in temperature of the extracellular solution from 22 to 38 °C on the mouse neuronal ASOR channel currents recorded at pH 5.25. The currents gradually decreased and increased with reducing and raising the temperature, respectively (Fig.…”

Section: Asor Anion Channel Is Expressed In Cortical Neurons and Is Tmentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9][10] This channel is phenotypically characterized by activation by severe extracellular acidification alone as well as by strong outward rectification [1][2][3][4][5][6][7][8][9][10] and was shown to be involved in the genesis of acidotoxic necrotic cell death in human epithelial cells. 1 Our recent study 11 showed that the epithelial ASOR anion channel is sensitive to temperature.…”

Section: Introductionmentioning

confidence: 99%

Temperature sensitivity of acid-sensitive outwardly rectifying (ASOR) anion channels in cortical neurons is involved in hypothermic neuroprotection against acidotoxic necrosis

2014

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Lower fecal pH may be a novel indicator of pouchitis after IPAA in patients with FAP or metachronous Lynch syndrome

Liu

Bao

Jiang

et al. 2019

Journal of Surgical Oncology

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Background and Objectives: To assess whether fecal pH might be an indicator of pouchitis during the postoperative period in hereditary colorectal cancer (CRC) patients who have undergone ileal pouch anal anastomosis (IPAA). Methods: Five consecutive daily pH values of stool samples from 31 familial adenomatous polyposis (FAP) patients and 32 metachronous Lynch syndrome patients who underwent IPAA procedures were reviewed. Patients with pouchitis (pouchitis group, n = 22) were compared with patients without pouchitis (nonpouchitis group, n = 41). A receiver operating characteristic (ROC) analysis was performed to determine the indicative potential of fecal pH for pouchitis. A Mantel-Cox test was also performed to evaluate the survival status of patients with or without pouchitis. Results: Pouchitis was noted in 22 (34.9%) of 63 patients after IPAA. The significance of each daily average fecal pH value and the 5-day overall average fecal pH value was compared between the two groups (P < .01). A cutoff fecal pH value of 7.46 was determined by the ROC analysis for assessing the risk of pouchitis. No significant difference in 5-year overall survival was observed between the two groups. Conclusion: A lower fecal pH value in patients with hereditary CRC after IPAA might be a new indicator of pouchitis. K E Y W O R D S hereditary colorectal cancer, IPAA, pH value, pouchitis

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Extracellular acidic pH-activated, outward rectifying chloride currents can be regulated by reactive oxygen species in human THP-1 monocytes

Cited by 14 publications

References 19 publications

Characterization of constitutive and acid-induced outwardly rectifying chloride currents in immortalized mouse distal tubular cells

Characterization of constitutive and acid-induced outwardly rectifying chloride currents in immortalized mouse distal tubular cells

Temperature sensitivity of acid-sensitive outwardly rectifying (ASOR) anion channels in cortical neurons is involved in hypothermic neuroprotection against acidotoxic necrosis

Lower fecal pH may be a novel indicator of pouchitis after IPAA in patients with FAP or metachronous Lynch syndrome

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